Molecular Detection and Genetic Characterization of Avian Leukosis Virus From Field Outbreaks in Bangladesh
ABSTRACT Avian leukosis is a significant viral disease affecting chicken populations globally, including Bangladesh, resulting in high mortality and morbidity rates and causing substantial economic losses in the commercial poultry industry. This study aimed to detect avian leukosis virus (ALV) durin...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-11-01
|
| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.70044 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832593428969422848 |
|---|---|
| author | Md. Golzar Hossain Riman Pathan SM Nazmul Hasan Anandha Mozumder Moslema Jahan Mou Marjana Akter Chandan Sikder Riyan Al Islam Reshad Roni Mia Sukumar Saha Tofazzal Islam Sharmin Akter |
| author_facet | Md. Golzar Hossain Riman Pathan SM Nazmul Hasan Anandha Mozumder Moslema Jahan Mou Marjana Akter Chandan Sikder Riyan Al Islam Reshad Roni Mia Sukumar Saha Tofazzal Islam Sharmin Akter |
| author_sort | Md. Golzar Hossain |
| collection | DOAJ |
| description | ABSTRACT Avian leukosis is a significant viral disease affecting chicken populations globally, including Bangladesh, resulting in high mortality and morbidity rates and causing substantial economic losses in the commercial poultry industry. This study aimed to detect avian leukosis virus (ALV) during recent outbreaks in Bangladesh, utilising a molecular‐based approach. A total of 14 liver samples were collected from the suspected layer flocks in Bangladesh. The diagnosis of ALV infection in chickens was confirmed through necropsy, histopathological examinations, reverse transcription‐polymerase chain reaction (RT‐PCR), and sequence analysis. Gross observations revealed severe liver enlargement with scattered white nodules on the surface in the infected chickens. Histopathological observations showed the infiltration of huge mononuclear inflammatory cells in the periportal area of liver and microvesicular fatty degeneration and necrosis of some hepatocytes. RT‐PCR results identified three samples positive for the env gene of ALV. Sequence analysis of the env genes demonstrated high homology among the identified strains (97%–98%) and with reference strains (92%–96%) at the nucleotide level. The phylogenetic tree revealed close relatedness of the three identified strains to reference strains from India, USA, and China. Mutational analysis indicated several mutations throughout the envelope glycoprotein of the identified strains. Protein structure analysis showed minor changes in the hydrophobic region of the envelope protein of the identified strains. In conclusion, this study, the first detailed investigation in Bangladesh, contributes to understanding ALV epidemiology, highlights genetic diversity, and emphasises the necessity for further investigations and the implementation of effective control measures in the affected regions. |
| format | Article |
| id | doaj-art-a50726b666844ab1a33f9d15aee3f3f2 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-a50726b666844ab1a33f9d15aee3f3f22025-01-20T17:16:44ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70044Molecular Detection and Genetic Characterization of Avian Leukosis Virus From Field Outbreaks in BangladeshMd. Golzar Hossain0Riman Pathan1SM Nazmul Hasan2Anandha Mozumder3Moslema Jahan Mou4Marjana Akter5Chandan Sikder6Riyan Al Islam Reshad7Roni Mia8Sukumar Saha9Tofazzal Islam10Sharmin Akter11Department of Microbiology and HygieneBangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and HygieneBangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and HygieneBangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and HygieneBangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and HygieneBangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and HygieneBangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and HygieneBangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and HygieneBangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and HygieneBangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and HygieneBangladesh Agricultural University Mymensingh BangladeshInstitute of Biotechnology and Genetic EngineeringBangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur BangladeshDepartment of PhysiologyBangladesh Agricultural UniversityMymensingh BangladeshABSTRACT Avian leukosis is a significant viral disease affecting chicken populations globally, including Bangladesh, resulting in high mortality and morbidity rates and causing substantial economic losses in the commercial poultry industry. This study aimed to detect avian leukosis virus (ALV) during recent outbreaks in Bangladesh, utilising a molecular‐based approach. A total of 14 liver samples were collected from the suspected layer flocks in Bangladesh. The diagnosis of ALV infection in chickens was confirmed through necropsy, histopathological examinations, reverse transcription‐polymerase chain reaction (RT‐PCR), and sequence analysis. Gross observations revealed severe liver enlargement with scattered white nodules on the surface in the infected chickens. Histopathological observations showed the infiltration of huge mononuclear inflammatory cells in the periportal area of liver and microvesicular fatty degeneration and necrosis of some hepatocytes. RT‐PCR results identified three samples positive for the env gene of ALV. Sequence analysis of the env genes demonstrated high homology among the identified strains (97%–98%) and with reference strains (92%–96%) at the nucleotide level. The phylogenetic tree revealed close relatedness of the three identified strains to reference strains from India, USA, and China. Mutational analysis indicated several mutations throughout the envelope glycoprotein of the identified strains. Protein structure analysis showed minor changes in the hydrophobic region of the envelope protein of the identified strains. In conclusion, this study, the first detailed investigation in Bangladesh, contributes to understanding ALV epidemiology, highlights genetic diversity, and emphasises the necessity for further investigations and the implementation of effective control measures in the affected regions.https://doi.org/10.1002/vms3.70044avian leukosis virusBangladeshgenetic characterizationgenome sequencemolecular detection |
| spellingShingle | Md. Golzar Hossain Riman Pathan SM Nazmul Hasan Anandha Mozumder Moslema Jahan Mou Marjana Akter Chandan Sikder Riyan Al Islam Reshad Roni Mia Sukumar Saha Tofazzal Islam Sharmin Akter Molecular Detection and Genetic Characterization of Avian Leukosis Virus From Field Outbreaks in Bangladesh Veterinary Medicine and Science avian leukosis virus Bangladesh genetic characterization genome sequence molecular detection |
| title | Molecular Detection and Genetic Characterization of Avian Leukosis Virus From Field Outbreaks in Bangladesh |
| title_full | Molecular Detection and Genetic Characterization of Avian Leukosis Virus From Field Outbreaks in Bangladesh |
| title_fullStr | Molecular Detection and Genetic Characterization of Avian Leukosis Virus From Field Outbreaks in Bangladesh |
| title_full_unstemmed | Molecular Detection and Genetic Characterization of Avian Leukosis Virus From Field Outbreaks in Bangladesh |
| title_short | Molecular Detection and Genetic Characterization of Avian Leukosis Virus From Field Outbreaks in Bangladesh |
| title_sort | molecular detection and genetic characterization of avian leukosis virus from field outbreaks in bangladesh |
| topic | avian leukosis virus Bangladesh genetic characterization genome sequence molecular detection |
| url | https://doi.org/10.1002/vms3.70044 |
| work_keys_str_mv | AT mdgolzarhossain moleculardetectionandgeneticcharacterizationofavianleukosisvirusfromfieldoutbreaksinbangladesh AT rimanpathan moleculardetectionandgeneticcharacterizationofavianleukosisvirusfromfieldoutbreaksinbangladesh AT smnazmulhasan moleculardetectionandgeneticcharacterizationofavianleukosisvirusfromfieldoutbreaksinbangladesh AT anandhamozumder moleculardetectionandgeneticcharacterizationofavianleukosisvirusfromfieldoutbreaksinbangladesh AT moslemajahanmou moleculardetectionandgeneticcharacterizationofavianleukosisvirusfromfieldoutbreaksinbangladesh AT marjanaakter moleculardetectionandgeneticcharacterizationofavianleukosisvirusfromfieldoutbreaksinbangladesh AT chandansikder moleculardetectionandgeneticcharacterizationofavianleukosisvirusfromfieldoutbreaksinbangladesh AT riyanalislamreshad moleculardetectionandgeneticcharacterizationofavianleukosisvirusfromfieldoutbreaksinbangladesh AT ronimia moleculardetectionandgeneticcharacterizationofavianleukosisvirusfromfieldoutbreaksinbangladesh AT sukumarsaha moleculardetectionandgeneticcharacterizationofavianleukosisvirusfromfieldoutbreaksinbangladesh AT tofazzalislam moleculardetectionandgeneticcharacterizationofavianleukosisvirusfromfieldoutbreaksinbangladesh AT sharminakter moleculardetectionandgeneticcharacterizationofavianleukosisvirusfromfieldoutbreaksinbangladesh |