Arecoline, a main alkaloid in Areca catechu, induces biological changes in human papillomavirus 16-positive cervical cancer cells via upregulation of viral oncogenes and cellular transcriptional factors
Objective: To investigate the effects of arecoline on HPV-positive cervical cells and unveil its underlying mechanism in cervical carcinogenesis. Methods: The cytotoxicity of arecoline was determined and the effect of subtoxic concentrations of arecoline on the expression of viral oncoproteins and t...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-03-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
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| Online Access: | https://journals.lww.com/10.4103/apjtb.apjtb_693_24 |
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| author | Jureeporn Chuerduangphui Chaleampol Loymunkong Tipaya Ekalaksananan Chamsai Pientong |
| author_facet | Jureeporn Chuerduangphui Chaleampol Loymunkong Tipaya Ekalaksananan Chamsai Pientong |
| author_sort | Jureeporn Chuerduangphui |
| collection | DOAJ |
| description | Objective:
To investigate the effects of arecoline on HPV-positive cervical cells and unveil its underlying mechanism in cervical carcinogenesis.
Methods:
The cytotoxicity of arecoline was determined and the effect of subtoxic concentrations of arecoline on the expression of viral oncoproteins and transcriptional factors was examined in CaSki and SiHa cells. HPV16 promoter activity was evaluated in a plasmid containing HPV16 long control region (pGL3-HPV16LCR)-transfected cells. Cell proliferation, cell migration, and number of colonies were assessed by MTT, wound healing assay, and colonyforming assay, respectively.
Results:
Arecoline at 0.01 μg/mL significantly upregulated HPV16 E6 and E7 oncoproteins in both CaSki and SiHa cells. It also upregulated the expression level of c-Fos and c-Jun mRNAs, and c-Myc protein in CaSki and SiHa cells. In addition, arecoline at subtoxic concentrations (0.002 5 and 0.01 μg/mL) significantly induced HPV16 promoter activity in pGL3-16LCR-transfected cells. It also promoted SiHa and CaSki cell proliferation, migration, and colony formation.
Conclusions:
Arecoline at subtoxic concentrations promotes the proliferation, migration, and colony formation of CaSki and SiHa cells via upregulation of c-Fos, c-Jun, c-Myc, and HPV16 E6 and E7 expressions. |
| format | Article |
| id | doaj-art-a4fd0a274bb947dca30a9faaf11f8744 |
| institution | DOAJ |
| issn | 2221-1691 2588-9222 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj-art-a4fd0a274bb947dca30a9faaf11f87442025-08-20T03:07:28ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222025-03-0115311912810.4103/apjtb.apjtb_693_24Arecoline, a main alkaloid in Areca catechu, induces biological changes in human papillomavirus 16-positive cervical cancer cells via upregulation of viral oncogenes and cellular transcriptional factorsJureeporn ChuerduangphuiChaleampol LoymunkongTipaya EkalaksanananChamsai PientongObjective: To investigate the effects of arecoline on HPV-positive cervical cells and unveil its underlying mechanism in cervical carcinogenesis. Methods: The cytotoxicity of arecoline was determined and the effect of subtoxic concentrations of arecoline on the expression of viral oncoproteins and transcriptional factors was examined in CaSki and SiHa cells. HPV16 promoter activity was evaluated in a plasmid containing HPV16 long control region (pGL3-HPV16LCR)-transfected cells. Cell proliferation, cell migration, and number of colonies were assessed by MTT, wound healing assay, and colonyforming assay, respectively. Results: Arecoline at 0.01 μg/mL significantly upregulated HPV16 E6 and E7 oncoproteins in both CaSki and SiHa cells. It also upregulated the expression level of c-Fos and c-Jun mRNAs, and c-Myc protein in CaSki and SiHa cells. In addition, arecoline at subtoxic concentrations (0.002 5 and 0.01 μg/mL) significantly induced HPV16 promoter activity in pGL3-16LCR-transfected cells. It also promoted SiHa and CaSki cell proliferation, migration, and colony formation. Conclusions: Arecoline at subtoxic concentrations promotes the proliferation, migration, and colony formation of CaSki and SiHa cells via upregulation of c-Fos, c-Jun, c-Myc, and HPV16 E6 and E7 expressions.https://journals.lww.com/10.4103/apjtb.apjtb_693_24arecolinehuman papillomavirusoncogenescervical cancerc-fos |
| spellingShingle | Jureeporn Chuerduangphui Chaleampol Loymunkong Tipaya Ekalaksananan Chamsai Pientong Arecoline, a main alkaloid in Areca catechu, induces biological changes in human papillomavirus 16-positive cervical cancer cells via upregulation of viral oncogenes and cellular transcriptional factors Asian Pacific Journal of Tropical Biomedicine arecoline human papillomavirus oncogenes cervical cancer c-fos |
| title | Arecoline, a main alkaloid in Areca catechu, induces biological changes in human papillomavirus 16-positive cervical cancer cells via upregulation of viral oncogenes and cellular transcriptional factors |
| title_full | Arecoline, a main alkaloid in Areca catechu, induces biological changes in human papillomavirus 16-positive cervical cancer cells via upregulation of viral oncogenes and cellular transcriptional factors |
| title_fullStr | Arecoline, a main alkaloid in Areca catechu, induces biological changes in human papillomavirus 16-positive cervical cancer cells via upregulation of viral oncogenes and cellular transcriptional factors |
| title_full_unstemmed | Arecoline, a main alkaloid in Areca catechu, induces biological changes in human papillomavirus 16-positive cervical cancer cells via upregulation of viral oncogenes and cellular transcriptional factors |
| title_short | Arecoline, a main alkaloid in Areca catechu, induces biological changes in human papillomavirus 16-positive cervical cancer cells via upregulation of viral oncogenes and cellular transcriptional factors |
| title_sort | arecoline a main alkaloid in areca catechu induces biological changes in human papillomavirus 16 positive cervical cancer cells via upregulation of viral oncogenes and cellular transcriptional factors |
| topic | arecoline human papillomavirus oncogenes cervical cancer c-fos |
| url | https://journals.lww.com/10.4103/apjtb.apjtb_693_24 |
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