The role and regulatory mechanism of miR-122-5p in the process of pilon fracture healing
Abstract Objective To explore the role and mechanism of miR-122-5p in the process of fracture healing following a Pilon fracture, as well as to further assess the clinical significance of this molecule as a potential diagnostic marker for fracture nonunion. Methods The expression levels of miR-122-5...
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BMC
2025-07-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | https://doi.org/10.1186/s13018-025-06120-4 |
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| author | Yang Zhang Yingnan Li Min Zhou Lichang Yang |
| author_facet | Yang Zhang Yingnan Li Min Zhou Lichang Yang |
| author_sort | Yang Zhang |
| collection | DOAJ |
| description | Abstract Objective To explore the role and mechanism of miR-122-5p in the process of fracture healing following a Pilon fracture, as well as to further assess the clinical significance of this molecule as a potential diagnostic marker for fracture nonunion. Methods The expression levels of miR-122-5p were examined in patients with Pilon fracture healed and nonunion, respectively. ROC analysis was employed to evaluate the diagnostic value of miR-122-5p in identifying fracture nonunion, while logistic regression identified its risk factors. The effects of miR-122-5p on the proliferation, apoptosis, and osteogenic differentiation markers of osteoblasts were analyzed by CCK-8 assays, flow cytometry, RT-qPCR, and ALP assay kit. Additionally, DLR and RIP assays validated miR-122-5p targeting PDCD4. Results Serum miR-122-5p was significantly under-expressed in patients with fracture nonunion, whereas PDCD4 exhibits a notable over-expressed. Serum miR-122-5p can predict non-union of fractures, with a sensitivity of 90.82% and a specificity of 78.33%. Low miR-122-5p expression is a potential risk factor for fracture nonunion. The overexpression of miR-122-5p enhanced the proliferation ability of osteoblasts, inhibited cell apoptosis, and upregulated the expressions of ALP, OCN, and RUNX2; knockdown of miR-122-5p elicits opposite effect. PDCD4 is a direct target of miR-122-5p. The osteoblast dysfunction induced by miR-122-5p inhibitors can be reversed by down-regulating PDCD4. Conclusion Diminished miR-122-5p emerge as a potential prognostic indicator for nonunion in Pilon fractures. miR-122-5p accelerates the healing of Pilon fractures by targeting and inhibiting PDCD4. |
| format | Article |
| id | doaj-art-a4f898deb285413083b5d9b1db661a6d |
| institution | Kabale University |
| issn | 1749-799X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
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| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj-art-a4f898deb285413083b5d9b1db661a6d2025-08-20T03:46:12ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2025-07-012011910.1186/s13018-025-06120-4The role and regulatory mechanism of miR-122-5p in the process of pilon fracture healingYang Zhang0Yingnan Li1Min Zhou2Lichang Yang3Faculty of Life science and Technology, Kunming University of Science and TechnologyFaculty of Life science and Technology, Kunming University of Science and TechnologyDepartment of Orthopedics and Traumatology, Wuxi Second Hospital of Traditional Chinese MedicineGraduate School, Nanjing University of Chinese MedicineAbstract Objective To explore the role and mechanism of miR-122-5p in the process of fracture healing following a Pilon fracture, as well as to further assess the clinical significance of this molecule as a potential diagnostic marker for fracture nonunion. Methods The expression levels of miR-122-5p were examined in patients with Pilon fracture healed and nonunion, respectively. ROC analysis was employed to evaluate the diagnostic value of miR-122-5p in identifying fracture nonunion, while logistic regression identified its risk factors. The effects of miR-122-5p on the proliferation, apoptosis, and osteogenic differentiation markers of osteoblasts were analyzed by CCK-8 assays, flow cytometry, RT-qPCR, and ALP assay kit. Additionally, DLR and RIP assays validated miR-122-5p targeting PDCD4. Results Serum miR-122-5p was significantly under-expressed in patients with fracture nonunion, whereas PDCD4 exhibits a notable over-expressed. Serum miR-122-5p can predict non-union of fractures, with a sensitivity of 90.82% and a specificity of 78.33%. Low miR-122-5p expression is a potential risk factor for fracture nonunion. The overexpression of miR-122-5p enhanced the proliferation ability of osteoblasts, inhibited cell apoptosis, and upregulated the expressions of ALP, OCN, and RUNX2; knockdown of miR-122-5p elicits opposite effect. PDCD4 is a direct target of miR-122-5p. The osteoblast dysfunction induced by miR-122-5p inhibitors can be reversed by down-regulating PDCD4. Conclusion Diminished miR-122-5p emerge as a potential prognostic indicator for nonunion in Pilon fractures. miR-122-5p accelerates the healing of Pilon fractures by targeting and inhibiting PDCD4.https://doi.org/10.1186/s13018-025-06120-4miR-122-5pPDCD4Pilon fracturesFracture healingOsteoblasts |
| spellingShingle | Yang Zhang Yingnan Li Min Zhou Lichang Yang The role and regulatory mechanism of miR-122-5p in the process of pilon fracture healing Journal of Orthopaedic Surgery and Research miR-122-5p PDCD4 Pilon fractures Fracture healing Osteoblasts |
| title | The role and regulatory mechanism of miR-122-5p in the process of pilon fracture healing |
| title_full | The role and regulatory mechanism of miR-122-5p in the process of pilon fracture healing |
| title_fullStr | The role and regulatory mechanism of miR-122-5p in the process of pilon fracture healing |
| title_full_unstemmed | The role and regulatory mechanism of miR-122-5p in the process of pilon fracture healing |
| title_short | The role and regulatory mechanism of miR-122-5p in the process of pilon fracture healing |
| title_sort | role and regulatory mechanism of mir 122 5p in the process of pilon fracture healing |
| topic | miR-122-5p PDCD4 Pilon fractures Fracture healing Osteoblasts |
| url | https://doi.org/10.1186/s13018-025-06120-4 |
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