PRDM16 acts as a homeostasis regulation factor to suppress the transition of AKI to CKD via upregulation of eukaryotic initiation factor 6
Abstract Progressing from acute kidney injury (AKI) to chronic kidney disease (CKD) is acknowledged as a significant clinical challenge. Our recent works indicated that PR domain-containing 16 (PRDM16) impedes the progression of AKI and DKD. Nonetheless, the specific function and regulatory mechanis...
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Springer
2025-06-01
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| Series: | Cellular and Molecular Life Sciences |
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| Online Access: | https://doi.org/10.1007/s00018-025-05766-x |
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| author | Xiaozhou Li Yang Xia Xingjin Li Yong Guo Shuangfa Qiu Huiling Li Yijian Li Lidong Wu Dongshan Zhang |
| author_facet | Xiaozhou Li Yang Xia Xingjin Li Yong Guo Shuangfa Qiu Huiling Li Yijian Li Lidong Wu Dongshan Zhang |
| author_sort | Xiaozhou Li |
| collection | DOAJ |
| description | Abstract Progressing from acute kidney injury (AKI) to chronic kidney disease (CKD) is acknowledged as a significant clinical challenge. Our recent works indicated that PR domain-containing 16 (PRDM16) impedes the progression of AKI and DKD. Nonetheless, the specific function and regulatory mechanism of PRDM16 during the AKI to CKD transition remain incompletely understood. In this investigation, it was identified that PRDM16 mitigates TGF-β1-induced renal tubulointerstitial fibrosis in BUMPT cells. From a mechanistic perspective, PRDM16 was found to enhance the expression of eif6, which subsequently suppressed TGF-β, CTGF, and NLRP3 levels via the suppression of the Wnt/β-catenin/SP1 signaling cascade. Additionally, knock-in of PRDM16 in kidney proximal tubules resulted in increased expression of eIF6, thereby restraining the stimulation of the Wnt/β-catenin/SP1 pathway, reducing the production of TGF-β, CTGF, and NLRP3, and consequently limiting renal tubulointerstitial fibrosis progression in both unilateral ureteral obstruction and ischemia–reperfusion-injury mouse models.Moreover, overexpression of PRDM16 following ischemia-induced AKI was shown to attenuate renal tubulointerstitial fibrosis and the eIF6/Wnt/β-catenin/SP1/TGF-β, CTGF, and NLRP3 axis. Finally, the PRDM16/eIF6/Wnt/β-catenin/SP1/TGF-β, CTGF, and NLRP3 axis were analyzed in renal biopsies from individuals with minimal change disease and severe obstructive nephropathy. Collectively, these findings indicate that PRDM16-mediated eIF6 induction serves to impede the transition from AKI to CKD by suppressing the Wnt/β-catenin/SP1/TGF-β, CTGF, and NLRP3 axis. |
| format | Article |
| id | doaj-art-a4e2c2cce8dd4e1da072a273ae0c9b2a |
| institution | Kabale University |
| issn | 1420-9071 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Springer |
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| series | Cellular and Molecular Life Sciences |
| spelling | doaj-art-a4e2c2cce8dd4e1da072a273ae0c9b2a2025-08-20T03:31:44ZengSpringerCellular and Molecular Life Sciences1420-90712025-06-0182111910.1007/s00018-025-05766-xPRDM16 acts as a homeostasis regulation factor to suppress the transition of AKI to CKD via upregulation of eukaryotic initiation factor 6Xiaozhou Li0Yang Xia1Xingjin Li2Yong Guo3Shuangfa Qiu4Huiling Li5Yijian Li6Lidong Wu7Dongshan Zhang8Department of Lntensive Care Medicine, Second Xiangya Hospital, Central South UniversityDepartment of Lntensive Care Medicine, Second Xiangya Hospital, Central South UniversityDepartment of Lntensive Care Medicine, Second Xiangya Hospital, Central South UniversityDepartment of Organ Procurement Organization, Second Xiangya Hospital, Central South UniversityDepartment of Emergency Medicine, Second Xiangya Hospital, Central South UniversityDepartment of Ophthalmology, Second Xiangya Hospital, Central South UniversityDepartment of Urology, Second Xiangya Hospital, Central South UniversityDepartment of Emergency Medicine, The Second Affiliated Hospital of Nanchang UniversitDepartment of Lntensive Care Medicine, Second Xiangya Hospital, Central South UniversityAbstract Progressing from acute kidney injury (AKI) to chronic kidney disease (CKD) is acknowledged as a significant clinical challenge. Our recent works indicated that PR domain-containing 16 (PRDM16) impedes the progression of AKI and DKD. Nonetheless, the specific function and regulatory mechanism of PRDM16 during the AKI to CKD transition remain incompletely understood. In this investigation, it was identified that PRDM16 mitigates TGF-β1-induced renal tubulointerstitial fibrosis in BUMPT cells. From a mechanistic perspective, PRDM16 was found to enhance the expression of eif6, which subsequently suppressed TGF-β, CTGF, and NLRP3 levels via the suppression of the Wnt/β-catenin/SP1 signaling cascade. Additionally, knock-in of PRDM16 in kidney proximal tubules resulted in increased expression of eIF6, thereby restraining the stimulation of the Wnt/β-catenin/SP1 pathway, reducing the production of TGF-β, CTGF, and NLRP3, and consequently limiting renal tubulointerstitial fibrosis progression in both unilateral ureteral obstruction and ischemia–reperfusion-injury mouse models.Moreover, overexpression of PRDM16 following ischemia-induced AKI was shown to attenuate renal tubulointerstitial fibrosis and the eIF6/Wnt/β-catenin/SP1/TGF-β, CTGF, and NLRP3 axis. Finally, the PRDM16/eIF6/Wnt/β-catenin/SP1/TGF-β, CTGF, and NLRP3 axis were analyzed in renal biopsies from individuals with minimal change disease and severe obstructive nephropathy. Collectively, these findings indicate that PRDM16-mediated eIF6 induction serves to impede the transition from AKI to CKD by suppressing the Wnt/β-catenin/SP1/TGF-β, CTGF, and NLRP3 axis.https://doi.org/10.1007/s00018-025-05766-xAKIPRDM16CKDeIF6 |
| spellingShingle | Xiaozhou Li Yang Xia Xingjin Li Yong Guo Shuangfa Qiu Huiling Li Yijian Li Lidong Wu Dongshan Zhang PRDM16 acts as a homeostasis regulation factor to suppress the transition of AKI to CKD via upregulation of eukaryotic initiation factor 6 Cellular and Molecular Life Sciences AKI PRDM16 CKD eIF6 |
| title | PRDM16 acts as a homeostasis regulation factor to suppress the transition of AKI to CKD via upregulation of eukaryotic initiation factor 6 |
| title_full | PRDM16 acts as a homeostasis regulation factor to suppress the transition of AKI to CKD via upregulation of eukaryotic initiation factor 6 |
| title_fullStr | PRDM16 acts as a homeostasis regulation factor to suppress the transition of AKI to CKD via upregulation of eukaryotic initiation factor 6 |
| title_full_unstemmed | PRDM16 acts as a homeostasis regulation factor to suppress the transition of AKI to CKD via upregulation of eukaryotic initiation factor 6 |
| title_short | PRDM16 acts as a homeostasis regulation factor to suppress the transition of AKI to CKD via upregulation of eukaryotic initiation factor 6 |
| title_sort | prdm16 acts as a homeostasis regulation factor to suppress the transition of aki to ckd via upregulation of eukaryotic initiation factor 6 |
| topic | AKI PRDM16 CKD eIF6 |
| url | https://doi.org/10.1007/s00018-025-05766-x |
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