Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells
The nonstructural protein 10 (nsp10) of porcine reproductive and respiratory syndrome virus (PRRSV) encodes for helicase which plays a vital role in viral replication. In the present study, a truncated form of nsp10, termed nsp10a, was found in PRRSV-infected cells and the production of nsp10a was s...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2018-05-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311917618963 |
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| author | Zhi-bang ZHANG Lei XU Xue-xia WEN Jian-guo DONG Lei ZHOU Xin-na GE Han-chun YANG Xin GUO |
| author_facet | Zhi-bang ZHANG Lei XU Xue-xia WEN Jian-guo DONG Lei ZHOU Xin-na GE Han-chun YANG Xin GUO |
| author_sort | Zhi-bang ZHANG |
| collection | DOAJ |
| description | The nonstructural protein 10 (nsp10) of porcine reproductive and respiratory syndrome virus (PRRSV) encodes for helicase which plays a vital role in viral replication. In the present study, a truncated form of nsp10, termed nsp10a, was found in PRRSV-infected cells and the production of nsp10a was strain-specific. Mass spectrometric analysis and deletion mutagenesis indicated that nsp10a may be short of about 70 amino acids in the N terminus of nsp10. Further studies by rescuing recombinant viruses showed that the Glu-69 in nsp10 was the key amino acid for nsp10a production. Finally, we demonstrated that nsp10a exerted little influence on the growth kinetics of PRRSV in vitro. |
| format | Article |
| id | doaj-art-a4e074e3f2774f29b55795e94652b935 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2018-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-a4e074e3f2774f29b55795e94652b9352025-08-20T03:56:58ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192018-05-011751171118010.1016/S2095-3119(17)61896-3Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cellsZhi-bang ZHANG0Lei XU1Xue-xia WEN2Jian-guo DONG3Lei ZHOU4Xin-na GE5Han-chun YANG6Xin GUO7ZHANG Zhi-bang, Tel: +86-10-62732875; Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. ChinaKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. ChinaKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. ChinaKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. ChinaKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. ChinaKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. ChinaCorrespondence YANG Han-chun, Tel/Fax: +86-10-62731296; Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. ChinaGUO Xin, Tel/Fax: +86-10-62732875; Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. ChinaThe nonstructural protein 10 (nsp10) of porcine reproductive and respiratory syndrome virus (PRRSV) encodes for helicase which plays a vital role in viral replication. In the present study, a truncated form of nsp10, termed nsp10a, was found in PRRSV-infected cells and the production of nsp10a was strain-specific. Mass spectrometric analysis and deletion mutagenesis indicated that nsp10a may be short of about 70 amino acids in the N terminus of nsp10. Further studies by rescuing recombinant viruses showed that the Glu-69 in nsp10 was the key amino acid for nsp10a production. Finally, we demonstrated that nsp10a exerted little influence on the growth kinetics of PRRSV in vitro.http://www.sciencedirect.com/science/article/pii/S2095311917618963porcine reproductive and respiratory syndrome virus (PRRSV)nonstructural protein 10 (nsp10)viral replication |
| spellingShingle | Zhi-bang ZHANG Lei XU Xue-xia WEN Jian-guo DONG Lei ZHOU Xin-na GE Han-chun YANG Xin GUO Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells Journal of Integrative Agriculture porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural protein 10 (nsp10) viral replication |
| title | Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells |
| title_full | Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells |
| title_fullStr | Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells |
| title_full_unstemmed | Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells |
| title_short | Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells |
| title_sort | identification of the strain specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells |
| topic | porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural protein 10 (nsp10) viral replication |
| url | http://www.sciencedirect.com/science/article/pii/S2095311917618963 |
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