Distributions of Lanostene-Derived Triterpenoids and Glucan Content in the Fruiting Bodies of the Australian <i>Ganoderma</i> Species

Lanostene-derived triterpenoids and β-glucans are important metabolites in <i>Ganoderma</i> mushrooms associated with benefits to human health. The medicinal value of the Australian <i>Ganoderma</i> species remains unclear, with no data on triterpenoid distribution or glucan...

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Main Authors: Aline De Oliveira Campos, Mark D. Harrison, David L. Marshall, Peter James Strong
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Journal of Fungi
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Online Access:https://www.mdpi.com/2309-608X/10/10/723
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author Aline De Oliveira Campos
Mark D. Harrison
David L. Marshall
Peter James Strong
author_facet Aline De Oliveira Campos
Mark D. Harrison
David L. Marshall
Peter James Strong
author_sort Aline De Oliveira Campos
collection DOAJ
description Lanostene-derived triterpenoids and β-glucans are important metabolites in <i>Ganoderma</i> mushrooms associated with benefits to human health. The medicinal value of the Australian <i>Ganoderma</i> species remains unclear, with no data on triterpenoid distribution or glucan content. In the present study, 22 Australian <i>Ganoderma</i> specimens were analyzed for triterpenoid and glucan contents. Thirty-two triterpenoids were identified in the fruiting bodies of 19 of the specimens. Distinct patterns in triterpenoid distribution between laccate and matte fruiting bodies were observed, leading to the classification of four groups of <i>Ganoderma</i>. Most of the glucans in the <i>Ganoderma</i> fruiting bodies were β-glucans (~99%), with a nominal α-glucan content (~1%). The β-glucan content ranged from 19.5 to 43.5% (<i>w</i>/<i>w</i>). A range of antioxidant activities was observed for methanol extracts using the ABTS (1.8 to 8.4 mg GAE.g<sup>−1</sup>), DPPH (1.7 to 9.4 mg GAE/g<sup>−1</sup>) and FRAP (24.7 to 111.6 mmol FeSO<sub>4</sub>.g<sup>−1</sup>) assays, with four specimens presenting relatively high radical scavenging and reducing activities. For the first time, we demonstrated that Australian <i>Ganoderma</i> mushrooms contain medicinal triterpenoids, including ganoderic acid A, and we established a link between its distribution and the fruiting body morphology. However, further research is required to isolate diploid clones and determine factors that impact triterpenoid and glucan synthesis in these strains.
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spelling doaj-art-a4da2e04124e482ab2bb8e2d0328202c2025-08-20T02:10:54ZengMDPI AGJournal of Fungi2309-608X2024-10-01101072310.3390/jof10100723Distributions of Lanostene-Derived Triterpenoids and Glucan Content in the Fruiting Bodies of the Australian <i>Ganoderma</i> SpeciesAline De Oliveira Campos0Mark D. Harrison1David L. Marshall2Peter James Strong3Center for Agriculture and the Bioeconomy, Queensland University of Technology, Brisbane 4000, AustraliaCenter for Agriculture and the Bioeconomy, Queensland University of Technology, Brisbane 4000, AustraliaCentral Analytical Research Facility, Queensland University of Technology, Brisbane 4000, AustraliaCenter for Agriculture and the Bioeconomy, Queensland University of Technology, Brisbane 4000, AustraliaLanostene-derived triterpenoids and β-glucans are important metabolites in <i>Ganoderma</i> mushrooms associated with benefits to human health. The medicinal value of the Australian <i>Ganoderma</i> species remains unclear, with no data on triterpenoid distribution or glucan content. In the present study, 22 Australian <i>Ganoderma</i> specimens were analyzed for triterpenoid and glucan contents. Thirty-two triterpenoids were identified in the fruiting bodies of 19 of the specimens. Distinct patterns in triterpenoid distribution between laccate and matte fruiting bodies were observed, leading to the classification of four groups of <i>Ganoderma</i>. Most of the glucans in the <i>Ganoderma</i> fruiting bodies were β-glucans (~99%), with a nominal α-glucan content (~1%). The β-glucan content ranged from 19.5 to 43.5% (<i>w</i>/<i>w</i>). A range of antioxidant activities was observed for methanol extracts using the ABTS (1.8 to 8.4 mg GAE.g<sup>−1</sup>), DPPH (1.7 to 9.4 mg GAE/g<sup>−1</sup>) and FRAP (24.7 to 111.6 mmol FeSO<sub>4</sub>.g<sup>−1</sup>) assays, with four specimens presenting relatively high radical scavenging and reducing activities. For the first time, we demonstrated that Australian <i>Ganoderma</i> mushrooms contain medicinal triterpenoids, including ganoderic acid A, and we established a link between its distribution and the fruiting body morphology. However, further research is required to isolate diploid clones and determine factors that impact triterpenoid and glucan synthesis in these strains.https://www.mdpi.com/2309-608X/10/10/723ganoderic acids<i>Ganoderma</i> mushroomsmass spectrometrymetabolitemorphologytriterpenoid
spellingShingle Aline De Oliveira Campos
Mark D. Harrison
David L. Marshall
Peter James Strong
Distributions of Lanostene-Derived Triterpenoids and Glucan Content in the Fruiting Bodies of the Australian <i>Ganoderma</i> Species
Journal of Fungi
ganoderic acids
<i>Ganoderma</i> mushrooms
mass spectrometry
metabolite
morphology
triterpenoid
title Distributions of Lanostene-Derived Triterpenoids and Glucan Content in the Fruiting Bodies of the Australian <i>Ganoderma</i> Species
title_full Distributions of Lanostene-Derived Triterpenoids and Glucan Content in the Fruiting Bodies of the Australian <i>Ganoderma</i> Species
title_fullStr Distributions of Lanostene-Derived Triterpenoids and Glucan Content in the Fruiting Bodies of the Australian <i>Ganoderma</i> Species
title_full_unstemmed Distributions of Lanostene-Derived Triterpenoids and Glucan Content in the Fruiting Bodies of the Australian <i>Ganoderma</i> Species
title_short Distributions of Lanostene-Derived Triterpenoids and Glucan Content in the Fruiting Bodies of the Australian <i>Ganoderma</i> Species
title_sort distributions of lanostene derived triterpenoids and glucan content in the fruiting bodies of the australian i ganoderma i species
topic ganoderic acids
<i>Ganoderma</i> mushrooms
mass spectrometry
metabolite
morphology
triterpenoid
url https://www.mdpi.com/2309-608X/10/10/723
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AT davidlmarshall distributionsoflanostenederivedtriterpenoidsandglucancontentinthefruitingbodiesoftheaustralianiganodermaispecies
AT peterjamesstrong distributionsoflanostenederivedtriterpenoidsandglucancontentinthefruitingbodiesoftheaustralianiganodermaispecies