Optimization of human TAT-Fab antibody expression and its bioactivity assay
In order to establish high effective expression system for human TAT-Fab antibody with protective activity, the induction conditions for the expression of TAT-Fab antibody in E. coli were optimized, and the bioactivity of antibody was assayed in vitro and in vivo. ELISA showed that human TAT-Fab ant...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2007-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2007.06.0651 |
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| Summary: | In order to establish high effective expression system for human TAT-Fab antibody with protective activity, the induction conditions for the expression of TAT-Fab antibody in E. coli were optimized, and the bioactivity of antibody was assayed in vitro and in vivo. ELISA showed that human TAT-Fab antibody expressed in E. coli possessed binding activity with tetanus toxin, and the expression level increased to nearly 5 times as compared with that before optimization. The expression products were ultra-filtered and analyzed by Western-blot, a specific band of TAT-Fab antibody, about 50 kDa, was detected. This antibody could neutralized tetanus toxin specifically and prolonged survival time of the mouse in vivo. The results indicate that human TAT-Fab antibody has the effect of neutralizing tetanus toxin in vitro and in vivo, which will provide experimental data for the production of human TAT antibody in the future. |
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| ISSN: | 1008-9209 2097-5155 |