Exploring Salivary Alpha-Amylase as a Biomarker in Periodontitis: A Comparative Analysis of Disease Stages and Clinical Correlations
Periodontal disease, characterized by bacterial plaque accumulation and subsequent immune response, can lead to gingivitis and periodontitis if untreated. Salivary alpha-amylase (sAA) has emerged as a potential biomarker with implications in periodontal disease progression. Objectives: This study ai...
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2024-10-01
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| author | Nada Tawfig Hashim Sadiah Fathima Nurain Mohammad Hisham Pooja Shivappa Michael V. Magaogao Md Sofiqul Islam Sara Faisal Ahmed Rasha Babiker Muhammed Mustahsen Rahman |
| author_facet | Nada Tawfig Hashim Sadiah Fathima Nurain Mohammad Hisham Pooja Shivappa Michael V. Magaogao Md Sofiqul Islam Sara Faisal Ahmed Rasha Babiker Muhammed Mustahsen Rahman |
| author_sort | Nada Tawfig Hashim |
| collection | DOAJ |
| description | Periodontal disease, characterized by bacterial plaque accumulation and subsequent immune response, can lead to gingivitis and periodontitis if untreated. Salivary alpha-amylase (sAA) has emerged as a potential biomarker with implications in periodontal disease progression. Objectives: This study aimed to assess and compare salivary alpha-amylase levels in individuals with periodontitis and healthy controls and to investigate its relationship with clinical parameters of periodontal disease. Forty-five participants were categorized into periodontally healthy (n = 13), Stage I and II Periodontitis (n = 17), and Stage III and IV periodontitis (n = 15) groups. Saliva samples were collected and analyzed using ELISA kits. Statistical analyses included tests for normality, group comparisons, post hoc analysis, and correlation analysis. Significant differences in salivary alpha-amylase levels were observed among severity groups (<i>p</i> < 0.05), with higher levels in periodontitis patients than healthy controls. Spearman correlation revealed moderate positive associations between alpha-amylase levels and probing depth (PD) and clinical attachment loss (CAL). Elevated salivary alpha-amylase levels were found to be associated with more severe periodontal disease, suggesting its potential as a biomarker for periodontitis severity. These findings support the utility of salivary biomarkers in periodontal disease diagnosis and monitoring, although further validation and standardization are warranted for clinical application. |
| format | Article |
| id | doaj-art-a4d45c8e6cf74c60b52011d531014c31 |
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| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-a4d45c8e6cf74c60b52011d531014c312025-08-20T02:08:03ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-10-014611122301224310.3390/cimb46110726Exploring Salivary Alpha-Amylase as a Biomarker in Periodontitis: A Comparative Analysis of Disease Stages and Clinical CorrelationsNada Tawfig Hashim0Sadiah Fathima1Nurain Mohammad Hisham2Pooja Shivappa3Michael V. Magaogao4Md Sofiqul Islam5Sara Faisal Ahmed6Rasha Babiker7Muhammed Mustahsen Rahman8Periodontics Department, RAK College of Dental Sciences, RAK Medical & Health Sciences University, Ras-AlKhaimah P.O. Box 12973, United Arab EmiratesPeriodontics Department, RAK College of Dental Sciences, RAK Medical & Health Sciences University, Ras-AlKhaimah P.O. Box 12973, United Arab EmiratesPeriodontics Department, RAK College of Dental Sciences, RAK Medical & Health Sciences University, Ras-AlKhaimah P.O. Box 12973, United Arab EmiratesTranslational Medical Research Centre, RAK Medical & Health Sciences University, Ras-AlKhaimah P.O. Box 11127, United Arab EmiratesTranslational Medical Research Centre, RAK Medical & Health Sciences University, Ras-AlKhaimah P.O. Box 11127, United Arab EmiratesOperative Department, RAK College of Dental Sciences, RAK Medical & Health Sciences University, Ras-AlKhaimah P.O. Box 12937, United Arab EmiratesDepartment of Statistics, Faculty of Mathematical Sciences, University of Khartoum, Khartoum 13314, SudanPhysiology Department, RAK College of Medical Sciences, RAK Medical & Health Sciences University, Ras-AlKhaimah P.O. Box 11127, United Arab EmiratesPeriodontics Department, RAK College of Dental Sciences, RAK Medical & Health Sciences University, Ras-AlKhaimah P.O. Box 12973, United Arab EmiratesPeriodontal disease, characterized by bacterial plaque accumulation and subsequent immune response, can lead to gingivitis and periodontitis if untreated. Salivary alpha-amylase (sAA) has emerged as a potential biomarker with implications in periodontal disease progression. Objectives: This study aimed to assess and compare salivary alpha-amylase levels in individuals with periodontitis and healthy controls and to investigate its relationship with clinical parameters of periodontal disease. Forty-five participants were categorized into periodontally healthy (n = 13), Stage I and II Periodontitis (n = 17), and Stage III and IV periodontitis (n = 15) groups. Saliva samples were collected and analyzed using ELISA kits. Statistical analyses included tests for normality, group comparisons, post hoc analysis, and correlation analysis. Significant differences in salivary alpha-amylase levels were observed among severity groups (<i>p</i> < 0.05), with higher levels in periodontitis patients than healthy controls. Spearman correlation revealed moderate positive associations between alpha-amylase levels and probing depth (PD) and clinical attachment loss (CAL). Elevated salivary alpha-amylase levels were found to be associated with more severe periodontal disease, suggesting its potential as a biomarker for periodontitis severity. These findings support the utility of salivary biomarkers in periodontal disease diagnosis and monitoring, although further validation and standardization are warranted for clinical application.https://www.mdpi.com/1467-3045/46/11/726periodontal diseasesalivary alpha-amylasebiomarkersperiodontitissaliva diagnosticsclinical attachment loss |
| spellingShingle | Nada Tawfig Hashim Sadiah Fathima Nurain Mohammad Hisham Pooja Shivappa Michael V. Magaogao Md Sofiqul Islam Sara Faisal Ahmed Rasha Babiker Muhammed Mustahsen Rahman Exploring Salivary Alpha-Amylase as a Biomarker in Periodontitis: A Comparative Analysis of Disease Stages and Clinical Correlations Current Issues in Molecular Biology periodontal disease salivary alpha-amylase biomarkers periodontitis saliva diagnostics clinical attachment loss |
| title | Exploring Salivary Alpha-Amylase as a Biomarker in Periodontitis: A Comparative Analysis of Disease Stages and Clinical Correlations |
| title_full | Exploring Salivary Alpha-Amylase as a Biomarker in Periodontitis: A Comparative Analysis of Disease Stages and Clinical Correlations |
| title_fullStr | Exploring Salivary Alpha-Amylase as a Biomarker in Periodontitis: A Comparative Analysis of Disease Stages and Clinical Correlations |
| title_full_unstemmed | Exploring Salivary Alpha-Amylase as a Biomarker in Periodontitis: A Comparative Analysis of Disease Stages and Clinical Correlations |
| title_short | Exploring Salivary Alpha-Amylase as a Biomarker in Periodontitis: A Comparative Analysis of Disease Stages and Clinical Correlations |
| title_sort | exploring salivary alpha amylase as a biomarker in periodontitis a comparative analysis of disease stages and clinical correlations |
| topic | periodontal disease salivary alpha-amylase biomarkers periodontitis saliva diagnostics clinical attachment loss |
| url | https://www.mdpi.com/1467-3045/46/11/726 |
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