Detection of Trypanosoma cruzi in white-eared opossums (Didelphis albiventris) from Canoinhas, Santa Catarina State, Brazil
Abstract Opossums are synanthropic animals that participate in the zoonotic transmission cycles. Chagas disease, a neglected tropical disease caused by Trypanosoma cruzi, affects many domestic and wild animals and humans worldwide. This study aimed to determine the occurrence of T. cruzi in free-ran...
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Colégio Brasileiro de Parasitologia Veterinaria
2025-02-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612025000100804&lng=en&tlng=en |
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| author | Giane Helenita Pontarolo Daniela Pedrassani Luís Felipe Kühl Monique Paiva Campos Thais Cristina Tirado Fabiano Borges Figueiredo Thállitha Samih Wischral Jayme Vieira Ana Cláudia Calchi Marcos Rogério André Rafael Felipe da Costa Vieira Ivan Roque de Barros Filho |
| author_facet | Giane Helenita Pontarolo Daniela Pedrassani Luís Felipe Kühl Monique Paiva Campos Thais Cristina Tirado Fabiano Borges Figueiredo Thállitha Samih Wischral Jayme Vieira Ana Cláudia Calchi Marcos Rogério André Rafael Felipe da Costa Vieira Ivan Roque de Barros Filho |
| author_sort | Giane Helenita Pontarolo |
| collection | DOAJ |
| description | Abstract Opossums are synanthropic animals that participate in the zoonotic transmission cycles. Chagas disease, a neglected tropical disease caused by Trypanosoma cruzi, affects many domestic and wild animals and humans worldwide. This study aimed to determine the occurrence of T. cruzi in free-ranging opossums in Canoinhas, Santa Catarina, Brazil. Fifty opossums (Didelphis albiventris) (33 captured and 17 road-killed) were evaluated using Nested-PCR assay. All tissue samples were negative (0/17). Eight of the 33 (24.24%; 95% CI:11.94–40,89%) blood samples were positive for T. cruzi. No significant associations were found between the sex (male/ female, p = 0.423), the trap area (rural/urban, p = 0.163), and positivity for T. cruzi in opossum blood samples. All samples showed 100% identity with T. cruzi (KF788250) isolated from Panstrongylus megistus in São Paulo, Brazil. The phylogenetic analysis model allocated all sequences obtained from D. albiventris to the large TcI clade of T. cruzi. This study provides the first record of T. cruzi in white-eared opossums in Canoinhas, Santa Catarina, southern Brazil. |
| format | Article |
| id | doaj-art-a4cfb9998a904afbbff9494bed1865a9 |
| institution | Kabale University |
| issn | 1984-2961 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj-art-a4cfb9998a904afbbff9494bed1865a92025-02-04T07:40:22ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612025-02-0134110.1590/s1984-29612025003Detection of Trypanosoma cruzi in white-eared opossums (Didelphis albiventris) from Canoinhas, Santa Catarina State, BrazilGiane Helenita Pontarolohttps://orcid.org/0000-0002-8449-1083Daniela Pedrassanihttps://orcid.org/0000-0001-6283-4981Luís Felipe KühlMonique Paiva Camposhttps://orcid.org/0000-0002-0082-0362Thais Cristina Tiradohttps://orcid.org/0000-0002-2368-7509Fabiano Borges Figueiredohttps://orcid.org/0000-0001-6861-0997Thállitha Samih Wischral Jayme Vieirahttps://orcid.org/0000-0002-8631-2712Ana Cláudia Calchihttps://orcid.org/0000-0002-4096-5937Marcos Rogério Andréhttps://orcid.org/0000-0002-1713-5222Rafael Felipe da Costa Vieirahttps://orcid.org/0000-0001-6613-0287Ivan Roque de Barros Filhohttps://orcid.org/0000-0002-0056-9358Abstract Opossums are synanthropic animals that participate in the zoonotic transmission cycles. Chagas disease, a neglected tropical disease caused by Trypanosoma cruzi, affects many domestic and wild animals and humans worldwide. This study aimed to determine the occurrence of T. cruzi in free-ranging opossums in Canoinhas, Santa Catarina, Brazil. Fifty opossums (Didelphis albiventris) (33 captured and 17 road-killed) were evaluated using Nested-PCR assay. All tissue samples were negative (0/17). Eight of the 33 (24.24%; 95% CI:11.94–40,89%) blood samples were positive for T. cruzi. No significant associations were found between the sex (male/ female, p = 0.423), the trap area (rural/urban, p = 0.163), and positivity for T. cruzi in opossum blood samples. All samples showed 100% identity with T. cruzi (KF788250) isolated from Panstrongylus megistus in São Paulo, Brazil. The phylogenetic analysis model allocated all sequences obtained from D. albiventris to the large TcI clade of T. cruzi. This study provides the first record of T. cruzi in white-eared opossums in Canoinhas, Santa Catarina, southern Brazil.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612025000100804&lng=en&tlng=enTrypanosoma cruziChagas diseaseDidelphis |
| spellingShingle | Giane Helenita Pontarolo Daniela Pedrassani Luís Felipe Kühl Monique Paiva Campos Thais Cristina Tirado Fabiano Borges Figueiredo Thállitha Samih Wischral Jayme Vieira Ana Cláudia Calchi Marcos Rogério André Rafael Felipe da Costa Vieira Ivan Roque de Barros Filho Detection of Trypanosoma cruzi in white-eared opossums (Didelphis albiventris) from Canoinhas, Santa Catarina State, Brazil Revista Brasileira de Parasitologia Veterinária Trypanosoma cruzi Chagas disease Didelphis |
| title | Detection of Trypanosoma cruzi in white-eared opossums (Didelphis albiventris) from Canoinhas, Santa Catarina State, Brazil |
| title_full | Detection of Trypanosoma cruzi in white-eared opossums (Didelphis albiventris) from Canoinhas, Santa Catarina State, Brazil |
| title_fullStr | Detection of Trypanosoma cruzi in white-eared opossums (Didelphis albiventris) from Canoinhas, Santa Catarina State, Brazil |
| title_full_unstemmed | Detection of Trypanosoma cruzi in white-eared opossums (Didelphis albiventris) from Canoinhas, Santa Catarina State, Brazil |
| title_short | Detection of Trypanosoma cruzi in white-eared opossums (Didelphis albiventris) from Canoinhas, Santa Catarina State, Brazil |
| title_sort | detection of trypanosoma cruzi in white eared opossums didelphis albiventris from canoinhas santa catarina state brazil |
| topic | Trypanosoma cruzi Chagas disease Didelphis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612025000100804&lng=en&tlng=en |
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