Stabilizing Water-in-Water Emulsions Using Oil Droplets
The production of water-in-water emulsion droplets, the coalescence of which is prevented by adding oil-in-water micrometric droplets, is reported. Hexadecane (O) and cetyl trimethyl ammonium bromide (CTAB) were added to a W/W emulsion made of dextran (Dex)-enriched droplets in a Polyethyleglycol (P...
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2025-07-01
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| author | Jean-Paul Douliez Laure Béven |
| author_facet | Jean-Paul Douliez Laure Béven |
| author_sort | Jean-Paul Douliez |
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| description | The production of water-in-water emulsion droplets, the coalescence of which is prevented by adding oil-in-water micrometric droplets, is reported. Hexadecane (O) and cetyl trimethyl ammonium bromide (CTAB) were added to a W/W emulsion made of dextran (Dex)-enriched droplets in a Polyethyleglycol (PEG)-enriched continuous phase, and the mixture was further sonicated. Using Nile red to label the oil droplets enabled the observation of their presence at the surface of Dex droplets (5 µm), allowing for stabilizing them, preventing coalescence of the W/W emulsion, and mimicking W/O/W double emulsions. The addition of sulfate derivative of Dextran (DexSulf) allowed for stable droplets of a slightly larger diameter. By contrast, the addition of carboxymethyl Dextran (CMDex) destabilized the initial aqueous double-like emulsion, yielding sequestration of the oil droplets within the Dex-rich phase. Interestingly, addition of DexSulf to that unstable emulsion re-yielded stable droplets. Similar findings (destabilization) were obtained when adding sodium dodecyl sulfate (SDS) to the initial double-like emulsion, which reformed stable droplets when adding positively charged Dextran (DEAEDex) derivatives. The use of fluorescently (FITC) labeled derivatives of Dextran (Dex, CMDex, DEAEDex, and DexSulf) allowed us to follow their position within, out of, or at the interface of droplets in the above-mentioned mixtures. These findings are expected to be of interest in the field of materials chemistry. |
| format | Article |
| id | doaj-art-a4bf39f5d63f43b2876ff2f52952a8ea |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-a4bf39f5d63f43b2876ff2f52952a8ea2025-08-20T03:36:31ZengMDPI AGMolecules1420-30492025-07-013015312010.3390/molecules30153120Stabilizing Water-in-Water Emulsions Using Oil DropletsJean-Paul Douliez0Laure Béven1Biologie du Fruit et Pathologie, INRAE, Univ. Bordeaux, UMR 1332, F-33140 Villenave d’Ornon, FranceBiologie du Fruit et Pathologie, INRAE, Univ. Bordeaux, UMR 1332, F-33140 Villenave d’Ornon, FranceThe production of water-in-water emulsion droplets, the coalescence of which is prevented by adding oil-in-water micrometric droplets, is reported. Hexadecane (O) and cetyl trimethyl ammonium bromide (CTAB) were added to a W/W emulsion made of dextran (Dex)-enriched droplets in a Polyethyleglycol (PEG)-enriched continuous phase, and the mixture was further sonicated. Using Nile red to label the oil droplets enabled the observation of their presence at the surface of Dex droplets (5 µm), allowing for stabilizing them, preventing coalescence of the W/W emulsion, and mimicking W/O/W double emulsions. The addition of sulfate derivative of Dextran (DexSulf) allowed for stable droplets of a slightly larger diameter. By contrast, the addition of carboxymethyl Dextran (CMDex) destabilized the initial aqueous double-like emulsion, yielding sequestration of the oil droplets within the Dex-rich phase. Interestingly, addition of DexSulf to that unstable emulsion re-yielded stable droplets. Similar findings (destabilization) were obtained when adding sodium dodecyl sulfate (SDS) to the initial double-like emulsion, which reformed stable droplets when adding positively charged Dextran (DEAEDex) derivatives. The use of fluorescently (FITC) labeled derivatives of Dextran (Dex, CMDex, DEAEDex, and DexSulf) allowed us to follow their position within, out of, or at the interface of droplets in the above-mentioned mixtures. These findings are expected to be of interest in the field of materials chemistry.https://www.mdpi.com/1420-3049/30/15/3120water-in-water emulsionstabilizationdouble emulsion |
| spellingShingle | Jean-Paul Douliez Laure Béven Stabilizing Water-in-Water Emulsions Using Oil Droplets Molecules water-in-water emulsion stabilization double emulsion |
| title | Stabilizing Water-in-Water Emulsions Using Oil Droplets |
| title_full | Stabilizing Water-in-Water Emulsions Using Oil Droplets |
| title_fullStr | Stabilizing Water-in-Water Emulsions Using Oil Droplets |
| title_full_unstemmed | Stabilizing Water-in-Water Emulsions Using Oil Droplets |
| title_short | Stabilizing Water-in-Water Emulsions Using Oil Droplets |
| title_sort | stabilizing water in water emulsions using oil droplets |
| topic | water-in-water emulsion stabilization double emulsion |
| url | https://www.mdpi.com/1420-3049/30/15/3120 |
| work_keys_str_mv | AT jeanpauldouliez stabilizingwaterinwateremulsionsusingoildroplets AT laurebeven stabilizingwaterinwateremulsionsusingoildroplets |