Carboxymethyl cellulose‐polylactic acid particles for inhibiting anoikis and enhancing wound healing efficacy of human mesenchymal stem cells
Abstract Adult human mesenchymal stem cells (hMSCs) injection into the wound site promotes angiogenesis and the wound‐closing process by secreting various growth and immune‐modulating factors. However, lower cell attachment sites and the hypoxic microenvironment in the wound site limit their viabili...
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| Format: | Article |
| Language: | English |
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Wiley
2025-07-01
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| Series: | Bioengineering & Translational Medicine |
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| Online Access: | https://doi.org/10.1002/btm2.70003 |
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| author | Dong‐Hyun Lee You Bin Lee Hyun Su Park Young‐Ju Jang Youn Chul Kim Suk Ho Bhang |
| author_facet | Dong‐Hyun Lee You Bin Lee Hyun Su Park Young‐Ju Jang Youn Chul Kim Suk Ho Bhang |
| author_sort | Dong‐Hyun Lee |
| collection | DOAJ |
| description | Abstract Adult human mesenchymal stem cells (hMSCs) injection into the wound site promotes angiogenesis and the wound‐closing process by secreting various growth and immune‐modulating factors. However, lower cell attachment sites and the hypoxic microenvironment in the wound site limit their viability and engraftment rate, leading to programmed cell death, anoikis. We synthesized carboxymethyl cellulose‐coated polylactic acid (CMC‐PLA) particles to prevent anoikis by providing an attachable surface for hMSCs. In vitro experiments demonstrated enhanced viability and secretion of growth factors by hMSCs under severely hypoxic microenvironments, when CMC‐PLA particles provided attachment surfaces, compared to controls. Furthermore, in vivo experiments showed that CMC‐PLA particles injected with hMSCs improved collagen synthesis and wound closure more than those of the control groups. These findings suggest that CMC‐PLA particles effectively enhance the therapeutic potential of hMSCs by providing a supportive microenvironment, promoting cell survival, proliferation, and angiogenesis, thereby offering a promising approach for advanced wound healing therapies. |
| format | Article |
| id | doaj-art-a4bda206fa7a4bcf83d864e55f8bae45 |
| institution | DOAJ |
| issn | 2380-6761 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj-art-a4bda206fa7a4bcf83d864e55f8bae452025-08-20T03:14:08ZengWileyBioengineering & Translational Medicine2380-67612025-07-01104n/an/a10.1002/btm2.70003Carboxymethyl cellulose‐polylactic acid particles for inhibiting anoikis and enhancing wound healing efficacy of human mesenchymal stem cellsDong‐Hyun Lee0You Bin Lee1Hyun Su Park2Young‐Ju Jang3Youn Chul Kim4Suk Ho Bhang5School of Chemical Engineering Sungkyunkwan University (SKKU) Suwon Gyeonggi‐do Republic of KoreaSchool of Chemical Engineering Sungkyunkwan University (SKKU) Suwon Gyeonggi‐do Republic of KoreaSchool of Chemical Engineering Sungkyunkwan University (SKKU) Suwon Gyeonggi‐do Republic of KoreaSchool of Chemical Engineering Sungkyunkwan University (SKKU) Suwon Gyeonggi‐do Republic of KoreaSchool of Chemical Engineering Sungkyunkwan University (SKKU) Suwon Gyeonggi‐do Republic of KoreaSchool of Chemical Engineering Sungkyunkwan University (SKKU) Suwon Gyeonggi‐do Republic of KoreaAbstract Adult human mesenchymal stem cells (hMSCs) injection into the wound site promotes angiogenesis and the wound‐closing process by secreting various growth and immune‐modulating factors. However, lower cell attachment sites and the hypoxic microenvironment in the wound site limit their viability and engraftment rate, leading to programmed cell death, anoikis. We synthesized carboxymethyl cellulose‐coated polylactic acid (CMC‐PLA) particles to prevent anoikis by providing an attachable surface for hMSCs. In vitro experiments demonstrated enhanced viability and secretion of growth factors by hMSCs under severely hypoxic microenvironments, when CMC‐PLA particles provided attachment surfaces, compared to controls. Furthermore, in vivo experiments showed that CMC‐PLA particles injected with hMSCs improved collagen synthesis and wound closure more than those of the control groups. These findings suggest that CMC‐PLA particles effectively enhance the therapeutic potential of hMSCs by providing a supportive microenvironment, promoting cell survival, proliferation, and angiogenesis, thereby offering a promising approach for advanced wound healing therapies.https://doi.org/10.1002/btm2.70003anoikiscarboxymethyl cellulosecell viabilitypolylactic acidstem cell therapy |
| spellingShingle | Dong‐Hyun Lee You Bin Lee Hyun Su Park Young‐Ju Jang Youn Chul Kim Suk Ho Bhang Carboxymethyl cellulose‐polylactic acid particles for inhibiting anoikis and enhancing wound healing efficacy of human mesenchymal stem cells Bioengineering & Translational Medicine anoikis carboxymethyl cellulose cell viability polylactic acid stem cell therapy |
| title | Carboxymethyl cellulose‐polylactic acid particles for inhibiting anoikis and enhancing wound healing efficacy of human mesenchymal stem cells |
| title_full | Carboxymethyl cellulose‐polylactic acid particles for inhibiting anoikis and enhancing wound healing efficacy of human mesenchymal stem cells |
| title_fullStr | Carboxymethyl cellulose‐polylactic acid particles for inhibiting anoikis and enhancing wound healing efficacy of human mesenchymal stem cells |
| title_full_unstemmed | Carboxymethyl cellulose‐polylactic acid particles for inhibiting anoikis and enhancing wound healing efficacy of human mesenchymal stem cells |
| title_short | Carboxymethyl cellulose‐polylactic acid particles for inhibiting anoikis and enhancing wound healing efficacy of human mesenchymal stem cells |
| title_sort | carboxymethyl cellulose polylactic acid particles for inhibiting anoikis and enhancing wound healing efficacy of human mesenchymal stem cells |
| topic | anoikis carboxymethyl cellulose cell viability polylactic acid stem cell therapy |
| url | https://doi.org/10.1002/btm2.70003 |
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