Microbiological analysis of a synbiotic gut health supplement capsule by metagenomics

Probiotics i.e. the use of living microorganisms for health benefits has been known since ancient times. There is an increasing awareness and concern among the consumers, regulators, medical fraternity, researchers, etc. about these products/supplements formulated to contain single or more benefici...

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Main Authors: Vivek N. Upasani, Shiva S. Chettiar, Alpesh R. Patel, Devendrasinh D. Jhala, Harsh Mistry
Format: Article
Language:English
Published: TMKarpinski Publisher, Tomasz M. Karpiński 2025-07-01
Series:European Journal of Biological Research
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Online Access:http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/767
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author Vivek N. Upasani
Shiva S. Chettiar
Alpesh R. Patel
Devendrasinh D. Jhala
Harsh Mistry
author_facet Vivek N. Upasani
Shiva S. Chettiar
Alpesh R. Patel
Devendrasinh D. Jhala
Harsh Mistry
author_sort Vivek N. Upasani
collection DOAJ
description Probiotics i.e. the use of living microorganisms for health benefits has been known since ancient times. There is an increasing awareness and concern among the consumers, regulators, medical fraternity, researchers, etc. about these products/supplements formulated to contain single or more beneficiary microorganisms. These microbes may be beneficial independently or synergistically for an individual’s health by protecting against pathogens, reduce metabolic disorders and boost immunity. Nowadays, next generation probiotics are being developed with microbial strains isolated from humans (gut or stool) or genetically engineered microbes for treating specific health condition. Gut microbiome studies have also helped in developing probiotics with novel microorganisms. Synbiotics are products containing prebiotics and probiotics. Food safety agencies provide guidelines to characterize and identify the microbial strains and their role in such products. Traditional, rapid identification and molecular methods as well as clinical trials are included to justify the efficacy and safety of the probiotics and related products. We report here the 16S metagenomics study on a synbiotic supplemental capsule available online containing twenty-five bacterial species. These results reveal the presence of a consortia of bacteria containing many species not mentioned on the product label. It also provides some insights for testing of these products using molecular tools for regulatory purpose. DOI: http://dx.doi.org/10.5281/zenodo.16543000
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institution Kabale University
issn 2449-8955
language English
publishDate 2025-07-01
publisher TMKarpinski Publisher, Tomasz M. Karpiński
record_format Article
series European Journal of Biological Research
spelling doaj-art-a4b7df5774aa4452a25bf8fa2ca4c9c52025-08-20T03:58:44ZengTMKarpinski Publisher, Tomasz M. KarpińskiEuropean Journal of Biological Research2449-89552025-07-01152Microbiological analysis of a synbiotic gut health supplement capsule by metagenomicsVivek N. Upasani0Shiva S. Chettiar1Alpesh R. Patel2Devendrasinh D. Jhala3Harsh Mistry4Genexplore Diagnostics and Research Centre Pvt. Ltd., 1201-1210, Iconic Shyamal, Shyamal Cross Road, Ahmedabad 380015, Gujarat, IndiaGenexplore Diagnostics and Research Centre Pvt. Ltd., 1201-1210, Iconic Shyamal, Shyamal Cross Road, Ahmedabad 380015, Gujarat, IndiaGenexplore Diagnostics and Research Centre Pvt. Ltd., 1201-1210, Iconic Shyamal, Shyamal Cross Road, Ahmedabad 380015, Gujarat, IndiaDepartment of Zoology, School of Sciences, Gujarat University, Ahmedabad 380009, Gujarat, IndiaGenexplore Diagnostics and Research Centre Pvt. Ltd., 1201-1210, Iconic Shyamal, Shyamal Cross Road, Ahmedabad 380015, Gujarat, India Probiotics i.e. the use of living microorganisms for health benefits has been known since ancient times. There is an increasing awareness and concern among the consumers, regulators, medical fraternity, researchers, etc. about these products/supplements formulated to contain single or more beneficiary microorganisms. These microbes may be beneficial independently or synergistically for an individual’s health by protecting against pathogens, reduce metabolic disorders and boost immunity. Nowadays, next generation probiotics are being developed with microbial strains isolated from humans (gut or stool) or genetically engineered microbes for treating specific health condition. Gut microbiome studies have also helped in developing probiotics with novel microorganisms. Synbiotics are products containing prebiotics and probiotics. Food safety agencies provide guidelines to characterize and identify the microbial strains and their role in such products. Traditional, rapid identification and molecular methods as well as clinical trials are included to justify the efficacy and safety of the probiotics and related products. We report here the 16S metagenomics study on a synbiotic supplemental capsule available online containing twenty-five bacterial species. These results reveal the presence of a consortia of bacteria containing many species not mentioned on the product label. It also provides some insights for testing of these products using molecular tools for regulatory purpose. DOI: http://dx.doi.org/10.5281/zenodo.16543000 http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/767ProbioticsMetagenomicsPrebioticsSynbioticsHealth supplements
spellingShingle Vivek N. Upasani
Shiva S. Chettiar
Alpesh R. Patel
Devendrasinh D. Jhala
Harsh Mistry
Microbiological analysis of a synbiotic gut health supplement capsule by metagenomics
European Journal of Biological Research
Probiotics
Metagenomics
Prebiotics
Synbiotics
Health supplements
title Microbiological analysis of a synbiotic gut health supplement capsule by metagenomics
title_full Microbiological analysis of a synbiotic gut health supplement capsule by metagenomics
title_fullStr Microbiological analysis of a synbiotic gut health supplement capsule by metagenomics
title_full_unstemmed Microbiological analysis of a synbiotic gut health supplement capsule by metagenomics
title_short Microbiological analysis of a synbiotic gut health supplement capsule by metagenomics
title_sort microbiological analysis of a synbiotic gut health supplement capsule by metagenomics
topic Probiotics
Metagenomics
Prebiotics
Synbiotics
Health supplements
url http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/767
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AT alpeshrpatel microbiologicalanalysisofasynbioticguthealthsupplementcapsulebymetagenomics
AT devendrasinhdjhala microbiologicalanalysisofasynbioticguthealthsupplementcapsulebymetagenomics
AT harshmistry microbiologicalanalysisofasynbioticguthealthsupplementcapsulebymetagenomics