Genetic diversity analysis and simple sequence repeat fingerprint construction of Acer truncatum bunge germplasm from udantara natural reserve, China
Acer truncatum Bunge is an ecologically and economically indigenous tree species in China, which is valued for ornamental, medicinal, and ecological contributions. As a major natural population of A. truncatum in China, the Udantara Natural Reserve harbors critical genetic resources for this specie...
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Universidade Federal de Uberlândia
2025-06-01
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| Series: | Bioscience Journal |
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| Online Access: | https://seer.ufu.br/index.php/biosciencejournal/article/view/74742 |
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| author | Zhiping Wu Xuefang Wang Minghai Han Chi Zhang Jun Wang Hasengaowa Bao |
| author_facet | Zhiping Wu Xuefang Wang Minghai Han Chi Zhang Jun Wang Hasengaowa Bao |
| author_sort | Zhiping Wu |
| collection | DOAJ |
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Acer truncatum Bunge is an ecologically and economically indigenous tree species in China, which is valued for ornamental, medicinal, and ecological contributions. As a major natural population of A. truncatum in China, the Udantara Natural Reserve harbors critical genetic resources for this species. To enhance genetic understanding and support conservation efforts, we analyzed the genetic diversity and population structure of 104 A. truncatum germplasm from Udantara using 20 pairs of simple sequence repeat (SSR) primers. Totally, 137 alleles were amplified, including 58.060 effective alleles. The average number of alleles (Na), effective number of alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and Shannon’s information index (I) were 6.850, 2.903, 0.424, 0.591, and 1.182, respectively, and the polymorphism information content (PIC) of the markers ranged between 0.293–0.839, with an average of 0.548, indicating moderate-to-high informativeness. The structure analysis partitioned the germplasm into two distinct genetic groups (28 and 76 individuals, respectively). Additionally, five SSR primers were identified as sufficient for generating unique genetic fingerprints for all 104 germplasm. Based on these findings, we propose a dual conservation strategy that integrates in situ protection of natural habitats within Udantara and ex situ preservation through a nationwide germplasm repository. Further recommendations include expanding the genetic representation by collecting germplasm across China and developing novel variants via hybridization and selective introduction. This study provides foundational genetic insights and actionable strategies to safeguard the biodiversity of A. truncatum, ensuring its ecological resilience and sustainable utilization in China.
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| format | Article |
| id | doaj-art-a4ac47d9d3784cd1aa051891df472387 |
| institution | OA Journals |
| issn | 1981-3163 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj-art-a4ac47d9d3784cd1aa051891df4723872025-08-20T02:36:02ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632025-06-014110.14393/BJ-v41n0a2025-74742Genetic diversity analysis and simple sequence repeat fingerprint construction of Acer truncatum bunge germplasm from udantara natural reserve, ChinaZhiping Wu0https://orcid.org/0009-0003-2815-9419Xuefang Wang1https://orcid.org/0009-0006-5392-7952Minghai Han2https://orcid.org/0009-0003-9497-0859Chi Zhang3https://orcid.org/0009-0001-1506-9217Jun Wang4https://orcid.org/0000-0003-0679-1125Hasengaowa Bao5https://orcid.org/0009-0006-9638-6017Beijing Forestry UniversityBeijing Forestry UniversityTongliao Forestry and Grassland Research InstituteTongliao Forestry and Grassland Research InstituteBeijing Forestry UniversityTongliao Forestry and Grassland Research Institute Acer truncatum Bunge is an ecologically and economically indigenous tree species in China, which is valued for ornamental, medicinal, and ecological contributions. As a major natural population of A. truncatum in China, the Udantara Natural Reserve harbors critical genetic resources for this species. To enhance genetic understanding and support conservation efforts, we analyzed the genetic diversity and population structure of 104 A. truncatum germplasm from Udantara using 20 pairs of simple sequence repeat (SSR) primers. Totally, 137 alleles were amplified, including 58.060 effective alleles. The average number of alleles (Na), effective number of alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and Shannon’s information index (I) were 6.850, 2.903, 0.424, 0.591, and 1.182, respectively, and the polymorphism information content (PIC) of the markers ranged between 0.293–0.839, with an average of 0.548, indicating moderate-to-high informativeness. The structure analysis partitioned the germplasm into two distinct genetic groups (28 and 76 individuals, respectively). Additionally, five SSR primers were identified as sufficient for generating unique genetic fingerprints for all 104 germplasm. Based on these findings, we propose a dual conservation strategy that integrates in situ protection of natural habitats within Udantara and ex situ preservation through a nationwide germplasm repository. Further recommendations include expanding the genetic representation by collecting germplasm across China and developing novel variants via hybridization and selective introduction. This study provides foundational genetic insights and actionable strategies to safeguard the biodiversity of A. truncatum, ensuring its ecological resilience and sustainable utilization in China. https://seer.ufu.br/index.php/biosciencejournal/article/view/74742Acer truncatum BungeFingerprintGenetic diversityPopulation structureSSR maker. |
| spellingShingle | Zhiping Wu Xuefang Wang Minghai Han Chi Zhang Jun Wang Hasengaowa Bao Genetic diversity analysis and simple sequence repeat fingerprint construction of Acer truncatum bunge germplasm from udantara natural reserve, China Bioscience Journal Acer truncatum Bunge Fingerprint Genetic diversity Population structure SSR maker. |
| title | Genetic diversity analysis and simple sequence repeat fingerprint construction of Acer truncatum bunge germplasm from udantara natural reserve, China |
| title_full | Genetic diversity analysis and simple sequence repeat fingerprint construction of Acer truncatum bunge germplasm from udantara natural reserve, China |
| title_fullStr | Genetic diversity analysis and simple sequence repeat fingerprint construction of Acer truncatum bunge germplasm from udantara natural reserve, China |
| title_full_unstemmed | Genetic diversity analysis and simple sequence repeat fingerprint construction of Acer truncatum bunge germplasm from udantara natural reserve, China |
| title_short | Genetic diversity analysis and simple sequence repeat fingerprint construction of Acer truncatum bunge germplasm from udantara natural reserve, China |
| title_sort | genetic diversity analysis and simple sequence repeat fingerprint construction of acer truncatum bunge germplasm from udantara natural reserve china |
| topic | Acer truncatum Bunge Fingerprint Genetic diversity Population structure SSR maker. |
| url | https://seer.ufu.br/index.php/biosciencejournal/article/view/74742 |
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