Greener solvents for microelution solid phase extraction of proteins from biological fluids followed by their top-down CE-MS analysis
In recent years, there has been a growing demand for making the analytical processes sustainable and environmentally friendly. In proteomic analysis, sample preparation plays an important role, employing different extraction techniques to isolate proteins of interest from biological fluids to be abl...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Advances in Sample Preparation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772582025000130 |
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| author | Martina Opetová Radovan Tomašovský Katarína Maráková |
| author_facet | Martina Opetová Radovan Tomašovský Katarína Maráková |
| author_sort | Martina Opetová |
| collection | DOAJ |
| description | In recent years, there has been a growing demand for making the analytical processes sustainable and environmentally friendly. In proteomic analysis, sample preparation plays an important role, employing different extraction techniques to isolate proteins of interest from biological fluids to be able to analyze them. These techniques usually use large volumes of organic solvents, such as acetonitrile, which has been recognized as hazardous and difficult to dispense. According to green analytical chemistry principles, the environmental impact of the methods can be reduced either by miniaturizing the processes or by replacing organic solvents with less toxic alternatives. This work compares the effect of different greener solvents on the solid-phase extraction process of low molecular weight proteins spiked in biological fluids (pooled human serum, plasma, urine, and saliva) and suggests that ethanol (60 %, v/v) offers, when used as an elution solvent, comparable results to acetonitrile (75 % v/v) in terms of extraction recovery. The addition of acidic modifiers to the elution solvent did not improve the extraction recovery, and the nitrogen evaporation of the eluate resulted in a 17–53 % loss of the proteins compared to the simple dilution of the eluate. |
| format | Article |
| id | doaj-art-a4abb91b8ab74d5b9fc533b7a562fb17 |
| institution | DOAJ |
| issn | 2772-5820 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Sample Preparation |
| spelling | doaj-art-a4abb91b8ab74d5b9fc533b7a562fb172025-08-20T03:11:06ZengElsevierAdvances in Sample Preparation2772-58202025-02-011310016010.1016/j.sampre.2025.100160Greener solvents for microelution solid phase extraction of proteins from biological fluids followed by their top-down CE-MS analysisMartina Opetová0Radovan Tomašovský1Katarína Maráková2Comenius University Bratislava, Faculty of Pharmacy, Department of Pharmaceutical Analysis and Nuclear Pharmacy, Odbojárov 10, SK-832 32, Bratislava, Slovakia; Comenius University Bratislava, Faculty of Pharmacy, Toxicological and Antidoping Center, Department of Pharmaceutical Analysis and Nuclear Pharmacy, Odbojárov 10, SK-832 32, Bratislava, SlovakiaComenius University Bratislava, Faculty of Pharmacy, Department of Pharmaceutical Analysis and Nuclear Pharmacy, Odbojárov 10, SK-832 32, Bratislava, Slovakia; Comenius University Bratislava, Faculty of Pharmacy, Toxicological and Antidoping Center, Department of Pharmaceutical Analysis and Nuclear Pharmacy, Odbojárov 10, SK-832 32, Bratislava, SlovakiaComenius University Bratislava, Faculty of Pharmacy, Department of Pharmaceutical Analysis and Nuclear Pharmacy, Odbojárov 10, SK-832 32, Bratislava, Slovakia; Comenius University Bratislava, Faculty of Pharmacy, Toxicological and Antidoping Center, Department of Pharmaceutical Analysis and Nuclear Pharmacy, Odbojárov 10, SK-832 32, Bratislava, Slovakia; Corresponding author.In recent years, there has been a growing demand for making the analytical processes sustainable and environmentally friendly. In proteomic analysis, sample preparation plays an important role, employing different extraction techniques to isolate proteins of interest from biological fluids to be able to analyze them. These techniques usually use large volumes of organic solvents, such as acetonitrile, which has been recognized as hazardous and difficult to dispense. According to green analytical chemistry principles, the environmental impact of the methods can be reduced either by miniaturizing the processes or by replacing organic solvents with less toxic alternatives. This work compares the effect of different greener solvents on the solid-phase extraction process of low molecular weight proteins spiked in biological fluids (pooled human serum, plasma, urine, and saliva) and suggests that ethanol (60 %, v/v) offers, when used as an elution solvent, comparable results to acetonitrile (75 % v/v) in terms of extraction recovery. The addition of acidic modifiers to the elution solvent did not improve the extraction recovery, and the nitrogen evaporation of the eluate resulted in a 17–53 % loss of the proteins compared to the simple dilution of the eluate.http://www.sciencedirect.com/science/article/pii/S2772582025000130Sample preparationCapillary electrophoresisIntact proteinsGreen solventsEthanol |
| spellingShingle | Martina Opetová Radovan Tomašovský Katarína Maráková Greener solvents for microelution solid phase extraction of proteins from biological fluids followed by their top-down CE-MS analysis Advances in Sample Preparation Sample preparation Capillary electrophoresis Intact proteins Green solvents Ethanol |
| title | Greener solvents for microelution solid phase extraction of proteins from biological fluids followed by their top-down CE-MS analysis |
| title_full | Greener solvents for microelution solid phase extraction of proteins from biological fluids followed by their top-down CE-MS analysis |
| title_fullStr | Greener solvents for microelution solid phase extraction of proteins from biological fluids followed by their top-down CE-MS analysis |
| title_full_unstemmed | Greener solvents for microelution solid phase extraction of proteins from biological fluids followed by their top-down CE-MS analysis |
| title_short | Greener solvents for microelution solid phase extraction of proteins from biological fluids followed by their top-down CE-MS analysis |
| title_sort | greener solvents for microelution solid phase extraction of proteins from biological fluids followed by their top down ce ms analysis |
| topic | Sample preparation Capillary electrophoresis Intact proteins Green solvents Ethanol |
| url | http://www.sciencedirect.com/science/article/pii/S2772582025000130 |
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