Polymorphism‐aware estimation of species trees and evolutionary forces from genomic sequences with RevBayes

Abstract The availability of population genomic data through new sequencing technologies gives unprecedented opportunities for estimating important evolutionary forces such as genetic drift, selection and mutation biases across organisms. Yet, analytical methods that can handle polymorphisms jointly...

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Main Authors: Rui Borges, Bastien Boussau, Sebastian Höhna, Ricardo J. Pereira, Carolin Kosiol
Format: Article
Language:English
Published: Wiley 2022-11-01
Series:Methods in Ecology and Evolution
Subjects:
Online Access:https://doi.org/10.1111/2041-210X.13980
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author Rui Borges
Bastien Boussau
Sebastian Höhna
Ricardo J. Pereira
Carolin Kosiol
author_facet Rui Borges
Bastien Boussau
Sebastian Höhna
Ricardo J. Pereira
Carolin Kosiol
author_sort Rui Borges
collection DOAJ
description Abstract The availability of population genomic data through new sequencing technologies gives unprecedented opportunities for estimating important evolutionary forces such as genetic drift, selection and mutation biases across organisms. Yet, analytical methods that can handle polymorphisms jointly with sequence divergence across species are rare and not easily accessible to empiricists. We implemented polymorphism‐aware phylogenetic models (PoMos), an alternative approach for species tree estimation, in the Bayesian phylogenetic software RevBayes. PoMos naturally account for incomplete lineage sorting, which is known to cause difficulties for phylogenetic inference in species radiations, and scale well with genome‐wide data. Simultaneously, PoMos can estimate mutation and selection biases. We have applied our methods to resolve the complex phylogenetic relationships of a young radiation of Chorthippus grasshoppers, based on coding sequences. In addition to establishing a well‐supported species tree, we found a mutation bias favouring AT alleles and selection bias promoting the fixation of GC alleles, the latter consistent with GC‐biased gene conversion. The selection bias is two orders of magnitude lower than genetic drift, validating the critical role of nearly neutral evolutionary processes in species radiation. PoMos offer a wide range of models to reconstruct phylogenies and can be easily combined with existing models in RevBayes—for example, relaxed clock and divergence time estimation—offering new insights into the evolutionary processes underlying molecular evolution and, ultimately, species diversification.
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publishDate 2022-11-01
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spelling doaj-art-a4a8db0f114d464a88b36d8aacbf2e222025-08-20T03:45:07ZengWileyMethods in Ecology and Evolution2041-210X2022-11-0113112339234610.1111/2041-210X.13980Polymorphism‐aware estimation of species trees and evolutionary forces from genomic sequences with RevBayesRui Borges0Bastien Boussau1Sebastian Höhna2Ricardo J. Pereira3Carolin Kosiol4Institut für Populationsgenetik, Vetmeduni Vienna Wien AustriaUniversité de Lyon, Université Claude Bernard Lyon 1 Villeurbanne FranceGeoBio‐Center, Ludwig‐Maximilians‐Universität München Munich GermanyDivision of Evolutionary Biology, Department of Biology II Ludwig‐Maximilians‐Universität München Martinsried GermanyInstitut für Populationsgenetik, Vetmeduni Vienna Wien AustriaAbstract The availability of population genomic data through new sequencing technologies gives unprecedented opportunities for estimating important evolutionary forces such as genetic drift, selection and mutation biases across organisms. Yet, analytical methods that can handle polymorphisms jointly with sequence divergence across species are rare and not easily accessible to empiricists. We implemented polymorphism‐aware phylogenetic models (PoMos), an alternative approach for species tree estimation, in the Bayesian phylogenetic software RevBayes. PoMos naturally account for incomplete lineage sorting, which is known to cause difficulties for phylogenetic inference in species radiations, and scale well with genome‐wide data. Simultaneously, PoMos can estimate mutation and selection biases. We have applied our methods to resolve the complex phylogenetic relationships of a young radiation of Chorthippus grasshoppers, based on coding sequences. In addition to establishing a well‐supported species tree, we found a mutation bias favouring AT alleles and selection bias promoting the fixation of GC alleles, the latter consistent with GC‐biased gene conversion. The selection bias is two orders of magnitude lower than genetic drift, validating the critical role of nearly neutral evolutionary processes in species radiation. PoMos offer a wide range of models to reconstruct phylogenies and can be easily combined with existing models in RevBayes—for example, relaxed clock and divergence time estimation—offering new insights into the evolutionary processes underlying molecular evolution and, ultimately, species diversification.https://doi.org/10.1111/2041-210X.13980Bayesian inferencegrasshoppersmutation biaspolymorphism‐aware phylogenetic modelsRevBayesselection
spellingShingle Rui Borges
Bastien Boussau
Sebastian Höhna
Ricardo J. Pereira
Carolin Kosiol
Polymorphism‐aware estimation of species trees and evolutionary forces from genomic sequences with RevBayes
Methods in Ecology and Evolution
Bayesian inference
grasshoppers
mutation bias
polymorphism‐aware phylogenetic models
RevBayes
selection
title Polymorphism‐aware estimation of species trees and evolutionary forces from genomic sequences with RevBayes
title_full Polymorphism‐aware estimation of species trees and evolutionary forces from genomic sequences with RevBayes
title_fullStr Polymorphism‐aware estimation of species trees and evolutionary forces from genomic sequences with RevBayes
title_full_unstemmed Polymorphism‐aware estimation of species trees and evolutionary forces from genomic sequences with RevBayes
title_short Polymorphism‐aware estimation of species trees and evolutionary forces from genomic sequences with RevBayes
title_sort polymorphism aware estimation of species trees and evolutionary forces from genomic sequences with revbayes
topic Bayesian inference
grasshoppers
mutation bias
polymorphism‐aware phylogenetic models
RevBayes
selection
url https://doi.org/10.1111/2041-210X.13980
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AT bastienboussau polymorphismawareestimationofspeciestreesandevolutionaryforcesfromgenomicsequenceswithrevbayes
AT sebastianhohna polymorphismawareestimationofspeciestreesandevolutionaryforcesfromgenomicsequenceswithrevbayes
AT ricardojpereira polymorphismawareestimationofspeciestreesandevolutionaryforcesfromgenomicsequenceswithrevbayes
AT carolinkosiol polymorphismawareestimationofspeciestreesandevolutionaryforcesfromgenomicsequenceswithrevbayes