An in silico to in vivo approach identifies retinoid-X receptor activating tert-butylphenols used in food contact materials

An in silico to in vivo approach identifies retinoid-X receptor activating tert-butylphenols used in food contact materials

Abstract The potential for food contact chemicals to disrupt genetic programs in development and metabolism raises concerns. Nuclear receptors (NRs) control many of these programs, and the retinoid-X receptor (RXR) is a DNA-binding partner for one-third of the NRs. RXR disruption could generate adve...

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Main Authors: Brenda J. Mengeling, Azhagiya Singam Ettayapuram Ramaprasad, Martyn T. Smith, Dania Turkieh, Nicole C. Kleinstreuer, Kamel Mansouri, Kathleen A. Durkin, Michele A. La Merrill, J. David Furlow
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
Subjects:
Retinoid-X receptor
Molecular docking
Environmental exposure
Food contact chemicals
Machine learning
Artificial intelligence
Online Access:https://doi.org/10.1038/s41598-025-09244-z
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Summary:Abstract The potential for food contact chemicals to disrupt genetic programs in development and metabolism raises concerns. Nuclear receptors (NRs) control many of these programs, and the retinoid-X receptor (RXR) is a DNA-binding partner for one-third of the NRs. RXR disruption could generate adverse outcomes in several NR pathways. We used machine learning and other in silico methods to identify RXR-interacting candidates from a list of over 57,000 chemicals. Butylphenols comprised the largest, high-probability, structural group (58 compounds); several are food contact chemicals with widespread commercial use. In vitro ToxCast data suggested that bulky, aliphatic substitution at C4 of 2,6-di-tert-butylphenol facilitated RXR activation. We tested six butylphenols with increasing bulk at C4 in vivo for their ability to disrupt thyroid hormone receptor (TR) signaling, using an integrated luciferase reporter driven by TR-RXR binding and quantifiable morphological changes in a Xenopus laevis precocious metamorphosis assay. Three tert-butylphenols potentiated TH action at nanomolar concentrations. Molecular modeling showed the three positives formed more frequent, stable interactions with RXRα, and bulkiness at C4 increased steric complementarity with the RXR ligand-binding pocket. Our findings establish a paradigm for machine learning coupled with a convenient, in vivo validation approach to identify chemicals interacting with RXR-NR-controlled genetic pathways.
ISSN:2045-2322

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