Exploring the antimicrobial potential of two haloarchaeal strains belonging to the genera Halopiger and Natrialba isolated from the Algerian Sahara
Background and Objectives: Halophilic archaea commonly produce antimicrobial peptides (halocins), but only a few studies have been conducted on these molecules. This study explores the antimicrobial potential of two strains belonging to Halopiger and Natrialba genera, isolated from hypersaline envi...
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Tehran University of Medical Sciences
2025-02-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4725 |
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| author | Inès Quadri Imene Ikram Hassani Hocine Hacène |
| author_facet | Inès Quadri Imene Ikram Hassani Hocine Hacène |
| author_sort | Inès Quadri |
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Background and Objectives: Halophilic archaea commonly produce antimicrobial peptides (halocins), but only a few studies have been conducted on these molecules. This study explores the antimicrobial potential of two strains belonging to Halopiger and Natrialba genera, isolated from hypersaline environments in the Algerian Sahara. Antimicrobial compounds produced by these genera have rarely been studied before.
Materials and Methods: The antimicrobial activity of the strains was evaluated, along with the effects of UV radiation and culture conditions on growth and compound production. Stability assays and the effects of extracted compounds on target cells and peripheral blood mononuclear cells (PBMC) were assessed.
Results: The strains exhibited high anti-archaeal activities and cross-domain interactions. Producing extracellular compounds associated with halocin, in the cell-free supernatant (CFS). These compounds remained stable at different temperatures (4°C, 60°C, 80°C, and 100°C) and different pH ranges (4-10 and 5-11), with antimicrobial profiles changed in response to UV light. The active compounds resembled known halocins but displayed unique features suggesting the discovery of new halocins. Additionally, Natrialba extracts showed significant activity against PBMC.
Conclusion: This investigation confirms that Algerian saline soils are a promising source of interesting antimicrobial compounds.
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| format | Article |
| id | doaj-art-a4a3bb58f80341cdb504ab53be209be6 |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-a4a3bb58f80341cdb504ab53be209be62025-02-09T09:01:23ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472025-02-01171Exploring the antimicrobial potential of two haloarchaeal strains belonging to the genera Halopiger and Natrialba isolated from the Algerian SaharaInès Quadri0Imene Ikram Hassani1Hocine Hacène2Department of Cellular and Molecular Biology, Cellular and Molecular Biology Laboratory (Team Microbiology), University of Sciences and Technology Houari Boumediene, Bab Ezzouar, Algiers, AlgeriaDepartment of Cellular and Molecular Biology, Cellular and Molecular Biology Laboratory (Team Microbiology), University of Sciences and Technology Houari Boumediene, Bab Ezzouar, Algiers, AlgeriaDepartment of Cellular and Molecular Biology, Cellular and Molecular Biology Laboratory (Team Microbiology), University of Sciences and Technology Houari Boumediene, Bab Ezzouar, Algiers, Algeria Background and Objectives: Halophilic archaea commonly produce antimicrobial peptides (halocins), but only a few studies have been conducted on these molecules. This study explores the antimicrobial potential of two strains belonging to Halopiger and Natrialba genera, isolated from hypersaline environments in the Algerian Sahara. Antimicrobial compounds produced by these genera have rarely been studied before. Materials and Methods: The antimicrobial activity of the strains was evaluated, along with the effects of UV radiation and culture conditions on growth and compound production. Stability assays and the effects of extracted compounds on target cells and peripheral blood mononuclear cells (PBMC) were assessed. Results: The strains exhibited high anti-archaeal activities and cross-domain interactions. Producing extracellular compounds associated with halocin, in the cell-free supernatant (CFS). These compounds remained stable at different temperatures (4°C, 60°C, 80°C, and 100°C) and different pH ranges (4-10 and 5-11), with antimicrobial profiles changed in response to UV light. The active compounds resembled known halocins but displayed unique features suggesting the discovery of new halocins. Additionally, Natrialba extracts showed significant activity against PBMC. Conclusion: This investigation confirms that Algerian saline soils are a promising source of interesting antimicrobial compounds. https://ijm.tums.ac.ir/index.php/ijm/article/view/4725Archaea;Halopiger;Natrialba;Extreme environments;Antimicrobial peptides;Peripheral blood mononuclear cells |
| spellingShingle | Inès Quadri Imene Ikram Hassani Hocine Hacène Exploring the antimicrobial potential of two haloarchaeal strains belonging to the genera Halopiger and Natrialba isolated from the Algerian Sahara Iranian Journal of Microbiology Archaea; Halopiger; Natrialba; Extreme environments; Antimicrobial peptides; Peripheral blood mononuclear cells |
| title | Exploring the antimicrobial potential of two haloarchaeal strains belonging to the genera Halopiger and Natrialba isolated from the Algerian Sahara |
| title_full | Exploring the antimicrobial potential of two haloarchaeal strains belonging to the genera Halopiger and Natrialba isolated from the Algerian Sahara |
| title_fullStr | Exploring the antimicrobial potential of two haloarchaeal strains belonging to the genera Halopiger and Natrialba isolated from the Algerian Sahara |
| title_full_unstemmed | Exploring the antimicrobial potential of two haloarchaeal strains belonging to the genera Halopiger and Natrialba isolated from the Algerian Sahara |
| title_short | Exploring the antimicrobial potential of two haloarchaeal strains belonging to the genera Halopiger and Natrialba isolated from the Algerian Sahara |
| title_sort | exploring the antimicrobial potential of two haloarchaeal strains belonging to the genera halopiger and natrialba isolated from the algerian sahara |
| topic | Archaea; Halopiger; Natrialba; Extreme environments; Antimicrobial peptides; Peripheral blood mononuclear cells |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4725 |
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