Atoh8 expression inhibition promoted osteogenic differentiation of ADSCs and inhibited cell proliferation in vitro and rat bone defect models
Stem cell-based bone tissue engineering offers a promising approach for treating oral and cranio-maxillofacial bone defects. This study investigated the role of Atoh8, a key regulator in various cells, in the osteogenic potential of adipose-derived stem cells (ADSCs). ADSCs transfected with small in...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Adipocyte |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21623945.2025.2494089 |
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| _version_ | 1850190253398163456 |
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| author | Zian Yi Shuang Song Yuxin Bai Guanhua Zhang Yuxi Wang Zijun Chen Xuefeng Chen Banglian Deng Xiangdong Liu Zuolin Jin |
| author_facet | Zian Yi Shuang Song Yuxin Bai Guanhua Zhang Yuxi Wang Zijun Chen Xuefeng Chen Banglian Deng Xiangdong Liu Zuolin Jin |
| author_sort | Zian Yi |
| collection | DOAJ |
| description | Stem cell-based bone tissue engineering offers a promising approach for treating oral and cranio-maxillofacial bone defects. This study investigated the role of Atoh8, a key regulator in various cells, in the osteogenic potential of adipose-derived stem cells (ADSCs). ADSCs transfected with small interfering RNA (siRNA) targeting Atoh8 were evaluated for proliferation, migration, adhesion, and osteogenic capacity. In vivo, 20 SD rats were used to assess bone regeneration using Atoh8-knockdown ADSC sheets, with new bone formation quantified via micro-CT and histological analysis. Atoh8 knockdown in vitro reduced ADSC proliferation and migration but enhanced osteogenic differentiation and upregulation of osteogenic-related factors. This approach improved bone healing in rat defect models, accelerating repair both in vitro and in vivo. The findings underscore the clinical potential of ADSCs in bone tissue engineering and elucidate Atoh8’s regulatory role in ADSC osteogenesis, providing a novel therapeutic strategy for enhancing bone regeneration through targeted modulation of stem cell differentiation pathways. |
| format | Article |
| id | doaj-art-a4a1b86f73e14d74bf1b3545b7496d19 |
| institution | OA Journals |
| issn | 2162-3945 2162-397X |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj-art-a4a1b86f73e14d74bf1b3545b7496d192025-08-20T02:15:20ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2025-12-0114110.1080/21623945.2025.2494089Atoh8 expression inhibition promoted osteogenic differentiation of ADSCs and inhibited cell proliferation in vitro and rat bone defect modelsZian Yi0Shuang Song1Yuxin Bai2Guanhua Zhang3Yuxi Wang4Zijun Chen5Xuefeng Chen6Banglian Deng7Xiangdong Liu8Zuolin Jin9State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, Air Force Medical University, Xi’an, ChinaKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Oral Implantology, School of Stomatology, Air Force Medical University, Xi’an, ChinaDepartment of Stomatology, Electric Power Teaching Hospital, Capital Medical University, Beijing, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Oral Implantology, School of Stomatology, Air Force Medical University, Xi’an, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Oral Implantology, School of Stomatology, Air Force Medical University, Xi’an, ChinaLianbang Research Institute of Oral Technology, Lianbang Hospital of Stomatology, Xi’an, ChinaLianbang Research Institute of Oral Technology, Lianbang Hospital of Stomatology, Xi’an, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Trauma and Orthognathic Surgery, School of Stomatology, Air Force Medical University, Xi’an, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, Air Force Medical University, Xi’an, ChinaStem cell-based bone tissue engineering offers a promising approach for treating oral and cranio-maxillofacial bone defects. This study investigated the role of Atoh8, a key regulator in various cells, in the osteogenic potential of adipose-derived stem cells (ADSCs). ADSCs transfected with small interfering RNA (siRNA) targeting Atoh8 were evaluated for proliferation, migration, adhesion, and osteogenic capacity. In vivo, 20 SD rats were used to assess bone regeneration using Atoh8-knockdown ADSC sheets, with new bone formation quantified via micro-CT and histological analysis. Atoh8 knockdown in vitro reduced ADSC proliferation and migration but enhanced osteogenic differentiation and upregulation of osteogenic-related factors. This approach improved bone healing in rat defect models, accelerating repair both in vitro and in vivo. The findings underscore the clinical potential of ADSCs in bone tissue engineering and elucidate Atoh8’s regulatory role in ADSC osteogenesis, providing a novel therapeutic strategy for enhancing bone regeneration through targeted modulation of stem cell differentiation pathways.https://www.tandfonline.com/doi/10.1080/21623945.2025.2494089Atoh8adipose-derived stem cellsosteogenic differentiationbone repairstem cell transplantation |
| spellingShingle | Zian Yi Shuang Song Yuxin Bai Guanhua Zhang Yuxi Wang Zijun Chen Xuefeng Chen Banglian Deng Xiangdong Liu Zuolin Jin Atoh8 expression inhibition promoted osteogenic differentiation of ADSCs and inhibited cell proliferation in vitro and rat bone defect models Adipocyte Atoh8 adipose-derived stem cells osteogenic differentiation bone repair stem cell transplantation |
| title | Atoh8 expression inhibition promoted osteogenic differentiation of ADSCs and inhibited cell proliferation in vitro and rat bone defect models |
| title_full | Atoh8 expression inhibition promoted osteogenic differentiation of ADSCs and inhibited cell proliferation in vitro and rat bone defect models |
| title_fullStr | Atoh8 expression inhibition promoted osteogenic differentiation of ADSCs and inhibited cell proliferation in vitro and rat bone defect models |
| title_full_unstemmed | Atoh8 expression inhibition promoted osteogenic differentiation of ADSCs and inhibited cell proliferation in vitro and rat bone defect models |
| title_short | Atoh8 expression inhibition promoted osteogenic differentiation of ADSCs and inhibited cell proliferation in vitro and rat bone defect models |
| title_sort | atoh8 expression inhibition promoted osteogenic differentiation of adscs and inhibited cell proliferation in vitro and rat bone defect models |
| topic | Atoh8 adipose-derived stem cells osteogenic differentiation bone repair stem cell transplantation |
| url | https://www.tandfonline.com/doi/10.1080/21623945.2025.2494089 |
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