Bioactivity of Ethanolic Extract of Propolis (EEP) in Balb/C Mice's CD4+CD25+ and B220+ Lymphocyte Cells
This experiment was aimed to determine the bioactivity of ethanolic extract of propolis (EEP) against the changes in the quantity of CD4+CD25+ and B220+ lymphocytes and determine the optimal dose of EEP to increase the number of CD4+CD25+ and B220+ cells in Balb/c mice. Balb/c mice were divided into...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2014-12-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/148 |
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| author | Aisyah Zahroh Aden Muhaimin Rifa'i |
| author_facet | Aisyah Zahroh Aden Muhaimin Rifa'i |
| author_sort | Aisyah Zahroh Aden |
| collection | DOAJ |
| description | This experiment was aimed to determine the bioactivity of ethanolic extract of propolis (EEP) against the changes in the quantity of CD4+CD25+ and B220+ lymphocytes and determine the optimal dose of EEP to increase the number of CD4+CD25+ and B220+ cells in Balb/c mice. Balb/c mice were divided into four treatment groups: control treatment, treatments of EEP at a dose of 50 mg.kg-1, 100 mg.kg-1, and 200 mg.kg-1 body weight of mice. All mice were dissected after two weeks post treatment. Profiles of lymphocytes from the spleen expressing CD4+CD25+ and B220+ cells were analyzed by flowcytometry using CellQuest software. Data was analyzed by Kruskall Wallis and Mann Whitney statistical test with P<0.05 using SPSS 16.0 for Windows. The results showed that the treatment of dose of 50 mg.kg-1 of EEP can increase relative number of CD4+CD25+ cells significantly, but those cells decrease significantly when we apply the dose of 100 and 200 mg.kg-1. The relative number of B220+ cells increase in the dose of 50 and 200 mg.kg-1 and decrease in the dose of 100 mg.kg-1 compared to the control. This experiment suggest that EEP has bioactivity to modulate the quantity of CD4+CD25+ and B220+ in dependent manner. |
| format | Article |
| id | doaj-art-a499508ea2d04fc08282c93d9e7a4213 |
| institution | OA Journals |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2014-12-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-a499508ea2d04fc08282c93d9e7a42132025-08-20T02:25:41ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552014-12-01423944https://doi.org/10.21776/ub.jels.2014.004.02.03Bioactivity of Ethanolic Extract of Propolis (EEP) in Balb/C Mice's CD4+CD25+ and B220+ Lymphocyte CellsAisyah Zahroh Aden0Muhaimin Rifa'i1Universitas Brawijaya, Malang, IndonesiaUniversitas Brawijaya, Malang, IndonesiaThis experiment was aimed to determine the bioactivity of ethanolic extract of propolis (EEP) against the changes in the quantity of CD4+CD25+ and B220+ lymphocytes and determine the optimal dose of EEP to increase the number of CD4+CD25+ and B220+ cells in Balb/c mice. Balb/c mice were divided into four treatment groups: control treatment, treatments of EEP at a dose of 50 mg.kg-1, 100 mg.kg-1, and 200 mg.kg-1 body weight of mice. All mice were dissected after two weeks post treatment. Profiles of lymphocytes from the spleen expressing CD4+CD25+ and B220+ cells were analyzed by flowcytometry using CellQuest software. Data was analyzed by Kruskall Wallis and Mann Whitney statistical test with P<0.05 using SPSS 16.0 for Windows. The results showed that the treatment of dose of 50 mg.kg-1 of EEP can increase relative number of CD4+CD25+ cells significantly, but those cells decrease significantly when we apply the dose of 100 and 200 mg.kg-1. The relative number of B220+ cells increase in the dose of 50 and 200 mg.kg-1 and decrease in the dose of 100 mg.kg-1 compared to the control. This experiment suggest that EEP has bioactivity to modulate the quantity of CD4+CD25+ and B220+ in dependent manner.https://jels.ub.ac.id/index.php/jels/article/view/148b220+cd4+cd25+lymphocytepropolis |
| spellingShingle | Aisyah Zahroh Aden Muhaimin Rifa'i Bioactivity of Ethanolic Extract of Propolis (EEP) in Balb/C Mice's CD4+CD25+ and B220+ Lymphocyte Cells Journal of Experimental Life Science b220+ cd4+cd25+ lymphocyte propolis |
| title | Bioactivity of Ethanolic Extract of Propolis (EEP) in Balb/C Mice's CD4+CD25+ and B220+ Lymphocyte Cells |
| title_full | Bioactivity of Ethanolic Extract of Propolis (EEP) in Balb/C Mice's CD4+CD25+ and B220+ Lymphocyte Cells |
| title_fullStr | Bioactivity of Ethanolic Extract of Propolis (EEP) in Balb/C Mice's CD4+CD25+ and B220+ Lymphocyte Cells |
| title_full_unstemmed | Bioactivity of Ethanolic Extract of Propolis (EEP) in Balb/C Mice's CD4+CD25+ and B220+ Lymphocyte Cells |
| title_short | Bioactivity of Ethanolic Extract of Propolis (EEP) in Balb/C Mice's CD4+CD25+ and B220+ Lymphocyte Cells |
| title_sort | bioactivity of ethanolic extract of propolis eep in balb c mice s cd4 cd25 and b220 lymphocyte cells |
| topic | b220+ cd4+cd25+ lymphocyte propolis |
| url | https://jels.ub.ac.id/index.php/jels/article/view/148 |
| work_keys_str_mv | AT aisyahzahrohaden bioactivityofethanolicextractofpropoliseepinbalbcmicescd4cd25andb220lymphocytecells AT muhaiminrifai bioactivityofethanolicextractofpropoliseepinbalbcmicescd4cd25andb220lymphocytecells |