In situ growth of β-galactosidase-manganese hybrid nanoflower on polycaprolactone/ xanthan electrospun nanofibers: A novel nanobiocatalyst for efficient lactose hydrolysis
Hydrolysis by β-galactosidase enzyme is an efficient and eco-friendly way to solve the problem of people with lactose intolerance. However, the activity reduction, instability, and high cost of β-galactosidase become the main limiting step for the practical application. In this study, a novel strate...
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Elsevier
2025-08-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154325004296 |
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| author | Shaghayegh Sheikhzadeh Mohammad Alizadeh Khaledabad Hadi Almasi |
| author_facet | Shaghayegh Sheikhzadeh Mohammad Alizadeh Khaledabad Hadi Almasi |
| author_sort | Shaghayegh Sheikhzadeh |
| collection | DOAJ |
| description | Hydrolysis by β-galactosidase enzyme is an efficient and eco-friendly way to solve the problem of people with lactose intolerance. However, the activity reduction, instability, and high cost of β-galactosidase become the main limiting step for the practical application. In this study, a novel strategy was suggested to improve this condition. For this purpose, the β-galactosidase/manganese hybrid nanoflower was successfully grown on the polycaprolactone (PCL)/xanthan electrospun nanofiber scaffold to achieve a highly active nanocomposite for lactose hydrolysis. To optimize the immobilization efficiency and find the optimal enzymatic conditions, the effect of four independent variables: enzyme concentration (0.1–0.9 mg/mL), manganese concentration (0.2–0.8 M), incubation time (2–18 h), and incubation temperature (5–39 °C) were investigated using a central composite design. The optimal conditions were determined as a manganese concentration of 0.63 M, β-galactosidase concentration of 0.57 mg/mL, incubation time of 12.6 h, and an incubation temperature of 23.5 °C, which resulted in an immobilization efficiency of 94.87 % and enzymatic activity of 60.23 mM. FESEM, EDS, FTIR, XRD, and TGA results confirmed the successful construction of manganese-based β-galactosidase hybrid nanoflowers on polycaprolactone/xanthan nanofibers. The optimum temperature and pH of the free enzyme were changed after immobilization. Immobilization caused a decrease in the Km and an increase in the Vmax. Also, nanoflower exhibited good stability and high reusability. Thus, the manganese hybrid nanoflowers on nanofibers are expected to be a promising support for biological molecule immobilization because of their hierarchical structures, high surface-to-volume ratio, easy separating, and excellent performance. |
| format | Article |
| id | doaj-art-a495f1d7fd304f32971a5fc4539699db |
| institution | Kabale University |
| issn | 2666-1543 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-a495f1d7fd304f32971a5fc4539699db2025-08-20T03:32:37ZengElsevierJournal of Agriculture and Food Research2666-15432025-08-012210205810.1016/j.jafr.2025.102058In situ growth of β-galactosidase-manganese hybrid nanoflower on polycaprolactone/ xanthan electrospun nanofibers: A novel nanobiocatalyst for efficient lactose hydrolysisShaghayegh Sheikhzadeh0Mohammad Alizadeh Khaledabad1Hadi Almasi2Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, IranCorresponding author.; Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, IranDepartment of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, IranHydrolysis by β-galactosidase enzyme is an efficient and eco-friendly way to solve the problem of people with lactose intolerance. However, the activity reduction, instability, and high cost of β-galactosidase become the main limiting step for the practical application. In this study, a novel strategy was suggested to improve this condition. For this purpose, the β-galactosidase/manganese hybrid nanoflower was successfully grown on the polycaprolactone (PCL)/xanthan electrospun nanofiber scaffold to achieve a highly active nanocomposite for lactose hydrolysis. To optimize the immobilization efficiency and find the optimal enzymatic conditions, the effect of four independent variables: enzyme concentration (0.1–0.9 mg/mL), manganese concentration (0.2–0.8 M), incubation time (2–18 h), and incubation temperature (5–39 °C) were investigated using a central composite design. The optimal conditions were determined as a manganese concentration of 0.63 M, β-galactosidase concentration of 0.57 mg/mL, incubation time of 12.6 h, and an incubation temperature of 23.5 °C, which resulted in an immobilization efficiency of 94.87 % and enzymatic activity of 60.23 mM. FESEM, EDS, FTIR, XRD, and TGA results confirmed the successful construction of manganese-based β-galactosidase hybrid nanoflowers on polycaprolactone/xanthan nanofibers. The optimum temperature and pH of the free enzyme were changed after immobilization. Immobilization caused a decrease in the Km and an increase in the Vmax. Also, nanoflower exhibited good stability and high reusability. Thus, the manganese hybrid nanoflowers on nanofibers are expected to be a promising support for biological molecule immobilization because of their hierarchical structures, high surface-to-volume ratio, easy separating, and excellent performance.http://www.sciencedirect.com/science/article/pii/S2666154325004296Polycaprolacton/Xanthan nanofiberOrganic-inorganic hybrid nanoflowerOptimizationMineralizationEnzyme immobilization |
| spellingShingle | Shaghayegh Sheikhzadeh Mohammad Alizadeh Khaledabad Hadi Almasi In situ growth of β-galactosidase-manganese hybrid nanoflower on polycaprolactone/ xanthan electrospun nanofibers: A novel nanobiocatalyst for efficient lactose hydrolysis Journal of Agriculture and Food Research Polycaprolacton/Xanthan nanofiber Organic-inorganic hybrid nanoflower Optimization Mineralization Enzyme immobilization |
| title | In situ growth of β-galactosidase-manganese hybrid nanoflower on polycaprolactone/ xanthan electrospun nanofibers: A novel nanobiocatalyst for efficient lactose hydrolysis |
| title_full | In situ growth of β-galactosidase-manganese hybrid nanoflower on polycaprolactone/ xanthan electrospun nanofibers: A novel nanobiocatalyst for efficient lactose hydrolysis |
| title_fullStr | In situ growth of β-galactosidase-manganese hybrid nanoflower on polycaprolactone/ xanthan electrospun nanofibers: A novel nanobiocatalyst for efficient lactose hydrolysis |
| title_full_unstemmed | In situ growth of β-galactosidase-manganese hybrid nanoflower on polycaprolactone/ xanthan electrospun nanofibers: A novel nanobiocatalyst for efficient lactose hydrolysis |
| title_short | In situ growth of β-galactosidase-manganese hybrid nanoflower on polycaprolactone/ xanthan electrospun nanofibers: A novel nanobiocatalyst for efficient lactose hydrolysis |
| title_sort | in situ growth of β galactosidase manganese hybrid nanoflower on polycaprolactone xanthan electrospun nanofibers a novel nanobiocatalyst for efficient lactose hydrolysis |
| topic | Polycaprolacton/Xanthan nanofiber Organic-inorganic hybrid nanoflower Optimization Mineralization Enzyme immobilization |
| url | http://www.sciencedirect.com/science/article/pii/S2666154325004296 |
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