Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens

Due to the use of antibiotics for the treatment and prevention of bacterial infections in intensive poultry production, it is important to study the prevalence and resistance profile of gram-negative extended-spectrum beta-lactamase (ESBL)-producing bacteria. Among the several classes of antibiotic...

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Main Authors: Taniara Suelen Mezalira, Daniela Dib Gonçalves, Eduardo Herrera Dias, Fábio Mendes da Silva, Isabela Carvalho dos Santos, Edson Gerônimo, Jaciele Caroline Pereira Dias, Luciana Kazue Otutumi
Format: Article
Language:English
Published: Universidade Federal de Santa Catarina 2019-12-01
Series:Biotemas
Online Access:https://periodicos.ufsc.br/index.php/biotemas/article/view/62068
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author Taniara Suelen Mezalira
Daniela Dib Gonçalves
Eduardo Herrera Dias
Fábio Mendes da Silva
Isabela Carvalho dos Santos
Edson Gerônimo
Jaciele Caroline Pereira Dias
Luciana Kazue Otutumi
author_facet Taniara Suelen Mezalira
Daniela Dib Gonçalves
Eduardo Herrera Dias
Fábio Mendes da Silva
Isabela Carvalho dos Santos
Edson Gerônimo
Jaciele Caroline Pereira Dias
Luciana Kazue Otutumi
author_sort Taniara Suelen Mezalira
collection DOAJ
description Due to the use of antibiotics for the treatment and prevention of bacterial infections in intensive poultry production, it is important to study the prevalence and resistance profile of gram-negative extended-spectrum beta-lactamase (ESBL)-producing bacteria. Among the several classes of antibiotics available, beta-lactam agents are the most widely used category in human and veterinary medicine. Thus, the objective of this work was to evaluate the resistance profile and ESBL-producing capacity of strains isolated from broiler breeders. Gram-negative bacteria were isolated in selective media from a collection of 87 cloacal swabs, and they were identified by conventional biochemical methods and MALDI-TOF mass spectrometry and studied for antibiotic resistance, including ESBL production. Of the 87 samples, 52 gram-negative bacteria were isolated. There was high bacterial resistance to the main classes of antibiotics used in poultry: cephalosporins (74.2%), quinolones (73.8%) and penicillins (73.6%). A phenotype suggestive of ESBL production was also found in 42.31% of isolates, showing resistance to the beta-lactam antibiotics tested. The results demonstrate the need for the guidance of professionals working broiler breeders to reduce the presence of drug-resistant and ESBL-producing bacteria and to prevent their spread to the environment, fertile eggs and broilers, and consequently to chicken meat and humans.
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institution Kabale University
issn 0103-1643
2175-7925
language English
publishDate 2019-12-01
publisher Universidade Federal de Santa Catarina
record_format Article
series Biotemas
spelling doaj-art-a48e8fd99f79466ea323ba43d477d9012025-08-20T03:39:44ZengUniversidade Federal de Santa CatarinaBiotemas0103-16432175-79252019-12-0132410.5007/2175-7925.2019v32n4p132540Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickensTaniara Suelen MezaliraDaniela Dib GonçalvesEduardo Herrera DiasFábio Mendes da SilvaIsabela Carvalho dos SantosEdson GerônimoJaciele Caroline Pereira DiasLuciana Kazue Otutumi Due to the use of antibiotics for the treatment and prevention of bacterial infections in intensive poultry production, it is important to study the prevalence and resistance profile of gram-negative extended-spectrum beta-lactamase (ESBL)-producing bacteria. Among the several classes of antibiotics available, beta-lactam agents are the most widely used category in human and veterinary medicine. Thus, the objective of this work was to evaluate the resistance profile and ESBL-producing capacity of strains isolated from broiler breeders. Gram-negative bacteria were isolated in selective media from a collection of 87 cloacal swabs, and they were identified by conventional biochemical methods and MALDI-TOF mass spectrometry and studied for antibiotic resistance, including ESBL production. Of the 87 samples, 52 gram-negative bacteria were isolated. There was high bacterial resistance to the main classes of antibiotics used in poultry: cephalosporins (74.2%), quinolones (73.8%) and penicillins (73.6%). A phenotype suggestive of ESBL production was also found in 42.31% of isolates, showing resistance to the beta-lactam antibiotics tested. The results demonstrate the need for the guidance of professionals working broiler breeders to reduce the presence of drug-resistant and ESBL-producing bacteria and to prevent their spread to the environment, fertile eggs and broilers, and consequently to chicken meat and humans. https://periodicos.ufsc.br/index.php/biotemas/article/view/62068
spellingShingle Taniara Suelen Mezalira
Daniela Dib Gonçalves
Eduardo Herrera Dias
Fábio Mendes da Silva
Isabela Carvalho dos Santos
Edson Gerônimo
Jaciele Caroline Pereira Dias
Luciana Kazue Otutumi
Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens
Biotemas
title Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens
title_full Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens
title_fullStr Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens
title_full_unstemmed Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens
title_short Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens
title_sort extended spectrum lactamase esbl producing enterobacteria isolated from broiler breeder chickens
url https://periodicos.ufsc.br/index.php/biotemas/article/view/62068
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