Graphene Derivatives as Efficient Transducing Materials for Covalent Immobilization of Biocomponents in Electrochemical Biosensors

This review highlights the role of graphene derivatives in advancing electrochemical biosensors for applications in diagnostics, environmental monitoring, and industrial sensing. Graphene derivatives, including graphene oxide (GO), reduced GO, and wide range of graphenes prepared via fluorographene...

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Main Authors: Petr Jakubec, David Panáček, Martin‐Alex Nalepa, Marianna Rossetti, Ruslan Álvarez‐Diduk, Arben Merkoçi, Majlinda Vasjari, Lueda Kulla, Michal Otyepka
Format: Article
Language:English
Published: Wiley-VCH 2025-06-01
Series:ChemElectroChem
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Online Access:https://doi.org/10.1002/celc.202400660
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author Petr Jakubec
David Panáček
Martin‐Alex Nalepa
Marianna Rossetti
Ruslan Álvarez‐Diduk
Arben Merkoçi
Majlinda Vasjari
Lueda Kulla
Michal Otyepka
author_facet Petr Jakubec
David Panáček
Martin‐Alex Nalepa
Marianna Rossetti
Ruslan Álvarez‐Diduk
Arben Merkoçi
Majlinda Vasjari
Lueda Kulla
Michal Otyepka
author_sort Petr Jakubec
collection DOAJ
description This review highlights the role of graphene derivatives in advancing electrochemical biosensors for applications in diagnostics, environmental monitoring, and industrial sensing. Graphene derivatives, including graphene oxide (GO), reduced GO, and wide range of graphenes prepared via fluorographene chemistry, represent a prominent class of transducing materials in electrochemical biosensor development. Their ability to support covalent immobilization of biocomponents ensures stability, specificity, and long‐term performance, addressing limitations of noncovalent methods. Advances in fabrication, such as laser‐assisted reduction, enable scalable and cost‐effective production of conductive graphene‐based electrodes. Covalent functionalization techniques, like carbodiimide coupling and click chemistry, facilitate integration with bioreceptors, leading to highly selective biosensors. Emerging approaches, including inkjet printing of graphene‐based inks onto eco‐friendly substrates, promise sustainable and portable diagnostic devices. These advances support biosensors aligned with modern and sustainable technologies. Future efforts must focus on scalable production, improved multiplexing, and environmental sustainability to fully harness the potential of graphene derivatives in electrochemical biosensors.
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institution OA Journals
issn 2196-0216
language English
publishDate 2025-06-01
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series ChemElectroChem
spelling doaj-art-a48650703c3c48cc87bc70d20341f4bc2025-08-20T02:33:12ZengWiley-VCHChemElectroChem2196-02162025-06-011212n/an/a10.1002/celc.202400660Graphene Derivatives as Efficient Transducing Materials for Covalent Immobilization of Biocomponents in Electrochemical BiosensorsPetr Jakubec0David Panáček1Martin‐Alex Nalepa2Marianna Rossetti3Ruslan Álvarez‐Diduk4Arben Merkoçi5Majlinda Vasjari6Lueda Kulla7Michal Otyepka8Regional Centre of Advanced Technologies and Materials Czech Advanced Technology and Research Institute (CATRIN) Palacký University Olomouc Šlechtitelů 241/27 783 71 Olomouc Czech RepublicRegional Centre of Advanced Technologies and Materials Czech Advanced Technology and Research Institute (CATRIN) Palacký University Olomouc Šlechtitelů 241/27 783 71 Olomouc Czech RepublicRegional Centre of Advanced Technologies and Materials Czech Advanced Technology and Research Institute (CATRIN) Palacký University Olomouc Šlechtitelů 241/27 783 71 Olomouc Czech RepublicCatalan Institute of Nanoscience and Nanotechnology (ICN2) CSIC and the Barcelona Institute of Science and Technology (BIST) Campus UAB, Bellaterra 08193 Barcelona SpainCatalan Institute of Nanoscience and Nanotechnology (ICN2) CSIC and the Barcelona Institute of Science and Technology (BIST) Campus UAB, Bellaterra 08193 Barcelona SpainCatalan Institute of Nanoscience and Nanotechnology (ICN2) CSIC and the Barcelona Institute of Science and Technology (BIST) Campus UAB, Bellaterra 08193 Barcelona SpainDepartment of Chemistry Faculty of Natural Sciences University of Tirana Tiranë 1000 AlbaniaDepartment of Chemistry Faculty of Natural Sciences University of Tirana Tiranë 1000 AlbaniaRegional Centre of Advanced Technologies and Materials Czech Advanced Technology and Research Institute (CATRIN) Palacký University Olomouc Šlechtitelů 241/27 783 71 Olomouc Czech RepublicThis review highlights the role of graphene derivatives in advancing electrochemical biosensors for applications in diagnostics, environmental monitoring, and industrial sensing. Graphene derivatives, including graphene oxide (GO), reduced GO, and wide range of graphenes prepared via fluorographene chemistry, represent a prominent class of transducing materials in electrochemical biosensor development. Their ability to support covalent immobilization of biocomponents ensures stability, specificity, and long‐term performance, addressing limitations of noncovalent methods. Advances in fabrication, such as laser‐assisted reduction, enable scalable and cost‐effective production of conductive graphene‐based electrodes. Covalent functionalization techniques, like carbodiimide coupling and click chemistry, facilitate integration with bioreceptors, leading to highly selective biosensors. Emerging approaches, including inkjet printing of graphene‐based inks onto eco‐friendly substrates, promise sustainable and portable diagnostic devices. These advances support biosensors aligned with modern and sustainable technologies. Future efforts must focus on scalable production, improved multiplexing, and environmental sustainability to fully harness the potential of graphene derivatives in electrochemical biosensors.https://doi.org/10.1002/celc.202400660biosensorscovalent immobilizationselectrochemistriesgraphenesinkjet printings
spellingShingle Petr Jakubec
David Panáček
Martin‐Alex Nalepa
Marianna Rossetti
Ruslan Álvarez‐Diduk
Arben Merkoçi
Majlinda Vasjari
Lueda Kulla
Michal Otyepka
Graphene Derivatives as Efficient Transducing Materials for Covalent Immobilization of Biocomponents in Electrochemical Biosensors
ChemElectroChem
biosensors
covalent immobilizations
electrochemistries
graphenes
inkjet printings
title Graphene Derivatives as Efficient Transducing Materials for Covalent Immobilization of Biocomponents in Electrochemical Biosensors
title_full Graphene Derivatives as Efficient Transducing Materials for Covalent Immobilization of Biocomponents in Electrochemical Biosensors
title_fullStr Graphene Derivatives as Efficient Transducing Materials for Covalent Immobilization of Biocomponents in Electrochemical Biosensors
title_full_unstemmed Graphene Derivatives as Efficient Transducing Materials for Covalent Immobilization of Biocomponents in Electrochemical Biosensors
title_short Graphene Derivatives as Efficient Transducing Materials for Covalent Immobilization of Biocomponents in Electrochemical Biosensors
title_sort graphene derivatives as efficient transducing materials for covalent immobilization of biocomponents in electrochemical biosensors
topic biosensors
covalent immobilizations
electrochemistries
graphenes
inkjet printings
url https://doi.org/10.1002/celc.202400660
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