Construction, visualisation, and clustering of transcription networks from microarray expression data.

Network analysis transcends conventional pairwise approaches to data analysis as the context of components in a network graph can be taken into account. Such approaches are increasingly being applied to genomics data, where functional linkages are used to connect genes or proteins. However, while mi...

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Main Authors: Tom C Freeman, Leon Goldovsky, Markus Brosch, Stijn van Dongen, Pierre Mazière, Russell J Grocock, Shiri Freilich, Janet Thornton, Anton J Enright
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2007-10-01
Series:PLoS Computational Biology
Online Access:https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.0030206&type=printable
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author Tom C Freeman
Leon Goldovsky
Markus Brosch
Stijn van Dongen
Pierre Mazière
Russell J Grocock
Shiri Freilich
Janet Thornton
Anton J Enright
author_facet Tom C Freeman
Leon Goldovsky
Markus Brosch
Stijn van Dongen
Pierre Mazière
Russell J Grocock
Shiri Freilich
Janet Thornton
Anton J Enright
author_sort Tom C Freeman
collection DOAJ
description Network analysis transcends conventional pairwise approaches to data analysis as the context of components in a network graph can be taken into account. Such approaches are increasingly being applied to genomics data, where functional linkages are used to connect genes or proteins. However, while microarray gene expression datasets are now abundant and of high quality, few approaches have been developed for analysis of such data in a network context. We present a novel approach for 3-D visualisation and analysis of transcriptional networks generated from microarray data. These networks consist of nodes representing transcripts connected by virtue of their expression profile similarity across multiple conditions. Analysing genome-wide gene transcription across 61 mouse tissues, we describe the unusual topography of the large and highly structured networks produced, and demonstrate how they can be used to visualise, cluster, and mine large datasets. This approach is fast, intuitive, and versatile, and allows the identification of biological relationships that may be missed by conventional analysis techniques. This work has been implemented in a freely available open-source application named BioLayout Express(3D).
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publishDate 2007-10-01
publisher Public Library of Science (PLoS)
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spelling doaj-art-a48133e434464c6c8d0abe7582a9a3092025-08-20T02:17:29ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582007-10-013102032204210.1371/journal.pcbi.0030206Construction, visualisation, and clustering of transcription networks from microarray expression data.Tom C FreemanLeon GoldovskyMarkus BroschStijn van DongenPierre MazièreRussell J GrocockShiri FreilichJanet ThorntonAnton J EnrightNetwork analysis transcends conventional pairwise approaches to data analysis as the context of components in a network graph can be taken into account. Such approaches are increasingly being applied to genomics data, where functional linkages are used to connect genes or proteins. However, while microarray gene expression datasets are now abundant and of high quality, few approaches have been developed for analysis of such data in a network context. We present a novel approach for 3-D visualisation and analysis of transcriptional networks generated from microarray data. These networks consist of nodes representing transcripts connected by virtue of their expression profile similarity across multiple conditions. Analysing genome-wide gene transcription across 61 mouse tissues, we describe the unusual topography of the large and highly structured networks produced, and demonstrate how they can be used to visualise, cluster, and mine large datasets. This approach is fast, intuitive, and versatile, and allows the identification of biological relationships that may be missed by conventional analysis techniques. This work has been implemented in a freely available open-source application named BioLayout Express(3D).https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.0030206&type=printable
spellingShingle Tom C Freeman
Leon Goldovsky
Markus Brosch
Stijn van Dongen
Pierre Mazière
Russell J Grocock
Shiri Freilich
Janet Thornton
Anton J Enright
Construction, visualisation, and clustering of transcription networks from microarray expression data.
PLoS Computational Biology
title Construction, visualisation, and clustering of transcription networks from microarray expression data.
title_full Construction, visualisation, and clustering of transcription networks from microarray expression data.
title_fullStr Construction, visualisation, and clustering of transcription networks from microarray expression data.
title_full_unstemmed Construction, visualisation, and clustering of transcription networks from microarray expression data.
title_short Construction, visualisation, and clustering of transcription networks from microarray expression data.
title_sort construction visualisation and clustering of transcription networks from microarray expression data
url https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.0030206&type=printable
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