Nitrogen‐Doped Multiwalled Carbon Nanotubes Trigger Immune Responses and Inhibit Fat Deposition
Abstract Multiwalled carbon nanotubes (MWCNTs) offer immense opportunities to deliver drugs and biomolecules to targeted tissues. However, it's unclear to their effects on fat metabolism. Here, it is demonstrated that nitrogen‐doped carboxylate‐functionalized MWCNTs (N‐MWCNTs) inhibit fat depos...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2024-12-01
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| Series: | Advanced Materials Interfaces |
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| Online Access: | https://doi.org/10.1002/admi.202400007 |
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| author | Dalin He Xue Xiao Geng Hu Wenqian Zhang Guanliu Yu Yan Liu Yun Lin Hai Lin Xianyao Li Youxiang Diao Yi Tang Haifang Li |
| author_facet | Dalin He Xue Xiao Geng Hu Wenqian Zhang Guanliu Yu Yan Liu Yun Lin Hai Lin Xianyao Li Youxiang Diao Yi Tang Haifang Li |
| author_sort | Dalin He |
| collection | DOAJ |
| description | Abstract Multiwalled carbon nanotubes (MWCNTs) offer immense opportunities to deliver drugs and biomolecules to targeted tissues. However, it's unclear to their effects on fat metabolism. Here, it is demonstrated that nitrogen‐doped carboxylate‐functionalized MWCNTs (N‐MWCNTs) inhibit fat deposition both in vivo and in vitro. N‐MWCNTs <0.5 µg mL−1 do not affect the viability of HEK293 cells and adipose‐derived stem cells (ASCs). Intramuscular administration of N‐MWCNTs does not affect the body weight gain and feed intake of mice, but reduces the fat mass. In in vitro‐cultured adipocytes, N‐MWCNTs suppress fat accumulation, accompanied by decreased and increased expression of adipogenic and lipolysis genes, respectively. Transcriptome analysis further certifies the N‐MWCNT alteration of fat metabolism‐related genes. Interestingly, the internalization of N‐MWCNTs by macrophage‐like cells via Transmission electron microscopy (TEM) imaging is observed. The mRNA sequencing data also shows remarkable variation of the genes involved in the Toll‐like receptors (TLRs) pathway, exhibiting down‐ or up‐regulation of inflammatory factors, of which TNF‐α, IL‐1, IL‐7, IL‐10, and IL‐12 are decreased, whereas IL‐6 and IL‐11 are increased. In conclusion, N‐MWCNTs trigger immune responses and reduction of fat deposition. |
| format | Article |
| id | doaj-art-a480808832ae49b49d36930d6987f82a |
| institution | DOAJ |
| issn | 2196-7350 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj-art-a480808832ae49b49d36930d6987f82a2025-08-20T02:52:27ZengWiley-VCHAdvanced Materials Interfaces2196-73502024-12-011136n/an/a10.1002/admi.202400007Nitrogen‐Doped Multiwalled Carbon Nanotubes Trigger Immune Responses and Inhibit Fat DepositionDalin He0Xue Xiao1Geng Hu2Wenqian Zhang3Guanliu Yu4Yan Liu5Yun Lin6Hai Lin7Xianyao Li8Youxiang Diao9Yi Tang10Haifang Li11College of Veterinary Medicine Shandong Agricultural University Tai'an 271018 ChinaCollege of Life Sciences Shandong Agricultural University Tai'an 271018 ChinaDepartment of Biotechnology Engineering Taishan Polytechnic College Tai'an 271018 ChinaCollege of Veterinary Medicine Shandong Agricultural University Tai'an 271018 ChinaCollege of Life Sciences Shandong Agricultural University Tai'an 271018 ChinaCollege of Life Sciences Shandong Agricultural University Tai'an 271018 ChinaCollege of Veterinary Medicine Shandong Agricultural University Tai'an 271018 ChinaCollege of Veterinary Medicine Shandong Agricultural University Tai'an 271018 ChinaCollege of Veterinary Medicine Shandong Agricultural University Tai'an 271018 ChinaCollege of Veterinary Medicine Shandong Agricultural University Tai'an 271018 ChinaCollege of Veterinary Medicine Shandong Agricultural University Tai'an 271018 ChinaCollege of Life Sciences Shandong Agricultural University Tai'an 271018 ChinaAbstract Multiwalled carbon nanotubes (MWCNTs) offer immense opportunities to deliver drugs and biomolecules to targeted tissues. However, it's unclear to their effects on fat metabolism. Here, it is demonstrated that nitrogen‐doped carboxylate‐functionalized MWCNTs (N‐MWCNTs) inhibit fat deposition both in vivo and in vitro. N‐MWCNTs <0.5 µg mL−1 do not affect the viability of HEK293 cells and adipose‐derived stem cells (ASCs). Intramuscular administration of N‐MWCNTs does not affect the body weight gain and feed intake of mice, but reduces the fat mass. In in vitro‐cultured adipocytes, N‐MWCNTs suppress fat accumulation, accompanied by decreased and increased expression of adipogenic and lipolysis genes, respectively. Transcriptome analysis further certifies the N‐MWCNT alteration of fat metabolism‐related genes. Interestingly, the internalization of N‐MWCNTs by macrophage‐like cells via Transmission electron microscopy (TEM) imaging is observed. The mRNA sequencing data also shows remarkable variation of the genes involved in the Toll‐like receptors (TLRs) pathway, exhibiting down‐ or up‐regulation of inflammatory factors, of which TNF‐α, IL‐1, IL‐7, IL‐10, and IL‐12 are decreased, whereas IL‐6 and IL‐11 are increased. In conclusion, N‐MWCNTs trigger immune responses and reduction of fat deposition.https://doi.org/10.1002/admi.202400007N‐MWCNTsfat depositionimmune responseTLRs pathway |
| spellingShingle | Dalin He Xue Xiao Geng Hu Wenqian Zhang Guanliu Yu Yan Liu Yun Lin Hai Lin Xianyao Li Youxiang Diao Yi Tang Haifang Li Nitrogen‐Doped Multiwalled Carbon Nanotubes Trigger Immune Responses and Inhibit Fat Deposition Advanced Materials Interfaces N‐MWCNTs fat deposition immune response TLRs pathway |
| title | Nitrogen‐Doped Multiwalled Carbon Nanotubes Trigger Immune Responses and Inhibit Fat Deposition |
| title_full | Nitrogen‐Doped Multiwalled Carbon Nanotubes Trigger Immune Responses and Inhibit Fat Deposition |
| title_fullStr | Nitrogen‐Doped Multiwalled Carbon Nanotubes Trigger Immune Responses and Inhibit Fat Deposition |
| title_full_unstemmed | Nitrogen‐Doped Multiwalled Carbon Nanotubes Trigger Immune Responses and Inhibit Fat Deposition |
| title_short | Nitrogen‐Doped Multiwalled Carbon Nanotubes Trigger Immune Responses and Inhibit Fat Deposition |
| title_sort | nitrogen doped multiwalled carbon nanotubes trigger immune responses and inhibit fat deposition |
| topic | N‐MWCNTs fat deposition immune response TLRs pathway |
| url | https://doi.org/10.1002/admi.202400007 |
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