Nitrogen‐Doped Multiwalled Carbon Nanotubes Trigger Immune Responses and Inhibit Fat Deposition

Nitrogen‐Doped Multiwalled Carbon Nanotubes Trigger Immune Responses and Inhibit Fat Deposition

Abstract Multiwalled carbon nanotubes (MWCNTs) offer immense opportunities to deliver drugs and biomolecules to targeted tissues. However, it's unclear to their effects on fat metabolism. Here, it is demonstrated that nitrogen‐doped carboxylate‐functionalized MWCNTs (N‐MWCNTs) inhibit fat depos...

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Bibliographic Details
Main Authors: Dalin He, Xue Xiao, Geng Hu, Wenqian Zhang, Guanliu Yu, Yan Liu, Yun Lin, Hai Lin, Xianyao Li, Youxiang Diao, Yi Tang, Haifang Li
Format: Article
Language:English
Published: Wiley-VCH 2024-12-01
Series:Advanced Materials Interfaces
Subjects:
N‐MWCNTs
fat deposition
immune response
TLRs pathway
Online Access:https://doi.org/10.1002/admi.202400007
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Summary:Abstract Multiwalled carbon nanotubes (MWCNTs) offer immense opportunities to deliver drugs and biomolecules to targeted tissues. However, it's unclear to their effects on fat metabolism. Here, it is demonstrated that nitrogen‐doped carboxylate‐functionalized MWCNTs (N‐MWCNTs) inhibit fat deposition both in vivo and in vitro. N‐MWCNTs <0.5 µg mL−1 do not affect the viability of HEK293 cells and adipose‐derived stem cells (ASCs). Intramuscular administration of N‐MWCNTs does not affect the body weight gain and feed intake of mice, but reduces the fat mass. In in vitro‐cultured adipocytes, N‐MWCNTs suppress fat accumulation, accompanied by decreased and increased expression of adipogenic and lipolysis genes, respectively. Transcriptome analysis further certifies the N‐MWCNT alteration of fat metabolism‐related genes. Interestingly, the internalization of N‐MWCNTs by macrophage‐like cells via Transmission electron microscopy (TEM) imaging is observed. The mRNA sequencing data also shows remarkable variation of the genes involved in the Toll‐like receptors (TLRs) pathway, exhibiting down‐ or up‐regulation of inflammatory factors, of which TNF‐α, IL‐1, IL‐7, IL‐10, and IL‐12 are decreased, whereas IL‐6 and IL‐11 are increased. In conclusion, N‐MWCNTs trigger immune responses and reduction of fat deposition.
ISSN:2196-7350

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