Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexus
Summary: Extracting the choroid plexus (ChP) for histology provides a comprehensive view of the whole tissue and is crucial for understanding its structure and cellular interactions. Here, we present a protocol for dissecting out the lateral, third, and fourth ventricle ChP from mice and the immunos...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000334 |
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| author | Lillian I.J. Byer Huixin Xu Maria K. Lehtinen |
| author_facet | Lillian I.J. Byer Huixin Xu Maria K. Lehtinen |
| author_sort | Lillian I.J. Byer |
| collection | DOAJ |
| description | Summary: Extracting the choroid plexus (ChP) for histology provides a comprehensive view of the whole tissue and is crucial for understanding its structure and cellular interactions. Here, we present a protocol for dissecting out the lateral, third, and fourth ventricle ChP from mice and the immunostaining process. We also detail procedures for preparing whole-mount ChP tissue and imaging techniques, such as tile scan and Z-stacks.For complete details on the use and execution of this protocol, please refer to Xu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-a4789f2dee8849f78c33d10ef236347a |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-a4789f2dee8849f78c33d10ef236347a2025-08-20T02:11:09ZengElsevierSTAR Protocols2666-16672025-03-016110362710.1016/j.xpro.2025.103627Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexusLillian I.J. Byer0Huixin Xu1Maria K. Lehtinen2Department of Pathology, Boston Children’s Hospital and Harvard Medical School, Boston, MA 02115, USA; Corresponding authorDepartment of Pathology, Boston Children’s Hospital and Harvard Medical School, Boston, MA 02115, USADepartment of Pathology, Boston Children’s Hospital and Harvard Medical School, Boston, MA 02115, USA; Corresponding authorSummary: Extracting the choroid plexus (ChP) for histology provides a comprehensive view of the whole tissue and is crucial for understanding its structure and cellular interactions. Here, we present a protocol for dissecting out the lateral, third, and fourth ventricle ChP from mice and the immunostaining process. We also detail procedures for preparing whole-mount ChP tissue and imaging techniques, such as tile scan and Z-stacks.For complete details on the use and execution of this protocol, please refer to Xu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000334cell biologyimmunologymicroscopyneuroscience |
| spellingShingle | Lillian I.J. Byer Huixin Xu Maria K. Lehtinen Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexus STAR Protocols cell biology immunology microscopy neuroscience |
| title | Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexus |
| title_full | Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexus |
| title_fullStr | Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexus |
| title_full_unstemmed | Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexus |
| title_short | Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexus |
| title_sort | protocol for the dissection immunostaining and imaging of whole mount mouse choroid plexus |
| topic | cell biology immunology microscopy neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000334 |
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