Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexus
Summary: Extracting the choroid plexus (ChP) for histology provides a comprehensive view of the whole tissue and is crucial for understanding its structure and cellular interactions. Here, we present a protocol for dissecting out the lateral, third, and fourth ventricle ChP from mice and the immunos...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000334 |
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| Summary: | Summary: Extracting the choroid plexus (ChP) for histology provides a comprehensive view of the whole tissue and is crucial for understanding its structure and cellular interactions. Here, we present a protocol for dissecting out the lateral, third, and fourth ventricle ChP from mice and the immunostaining process. We also detail procedures for preparing whole-mount ChP tissue and imaging techniques, such as tile scan and Z-stacks.For complete details on the use and execution of this protocol, please refer to Xu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| ISSN: | 2666-1667 |