Transcriptomic analysis reveals the inhibitory effect of beta-sitosterol on proliferation of bovine preadipocytes
Fat deposition affects beef quantity and quality via preadipocyte proliferation. Beta-sitosterol, a natural small molecular compound, has various functions, such as anti-inflammation, antibacterial, and anticancer properties. The mechanism of action of Beta-sitosterol on bovine preadipocytes remains...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2024.2339406 |
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| _version_ | 1846099270524993536 |
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| author | Lei Jiang Yuan Wan Jinhai Pan Xiaoyu Mao Xiaolei Sun Linsen Zan Hongbao Wang |
| author_facet | Lei Jiang Yuan Wan Jinhai Pan Xiaoyu Mao Xiaolei Sun Linsen Zan Hongbao Wang |
| author_sort | Lei Jiang |
| collection | DOAJ |
| description | Fat deposition affects beef quantity and quality via preadipocyte proliferation. Beta-sitosterol, a natural small molecular compound, has various functions, such as anti-inflammation, antibacterial, and anticancer properties. The mechanism of action of Beta-sitosterol on bovine preadipocytes remains unclear. This study, based on RNA-seq, reveals the impact of Beta -sitosterol on the proliferation of bovine preadipocytes. Compared to the control group, Beta-sitosterol demonstrated a more pronounced inhibitory effect on cell proliferation after 48 hours of treatment than after 24 hours, as evidenced by the results of EdU staining and flow cytometry. RNA-seq and Western Blot analyses further substantiated these findings. Our results suggest that the impact of Beta-sitosterol on the proliferation of bovine preadipocytes is not significant after a 24-hour treatment. It is only after extending the treatment time to 48 hours that Beta-sitosterol may induce cell cycle arrest at the G2/M phase by suppressing the expression of CCNB1, thereby inhibiting the proliferation of bovine preadipocytes. |
| format | Article |
| id | doaj-art-a475f7de49ea4d06b630f7f9b95585ad |
| institution | Kabale University |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-a475f7de49ea4d06b630f7f9b95585ad2024-12-31T18:28:13ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2024.2339406Transcriptomic analysis reveals the inhibitory effect of beta-sitosterol on proliferation of bovine preadipocytesLei Jiang0Yuan Wan1Jinhai Pan2Xiaoyu Mao3Xiaolei Sun4Linsen Zan5Hongbao Wang6College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, ChinaFat deposition affects beef quantity and quality via preadipocyte proliferation. Beta-sitosterol, a natural small molecular compound, has various functions, such as anti-inflammation, antibacterial, and anticancer properties. The mechanism of action of Beta-sitosterol on bovine preadipocytes remains unclear. This study, based on RNA-seq, reveals the impact of Beta -sitosterol on the proliferation of bovine preadipocytes. Compared to the control group, Beta-sitosterol demonstrated a more pronounced inhibitory effect on cell proliferation after 48 hours of treatment than after 24 hours, as evidenced by the results of EdU staining and flow cytometry. RNA-seq and Western Blot analyses further substantiated these findings. Our results suggest that the impact of Beta-sitosterol on the proliferation of bovine preadipocytes is not significant after a 24-hour treatment. It is only after extending the treatment time to 48 hours that Beta-sitosterol may induce cell cycle arrest at the G2/M phase by suppressing the expression of CCNB1, thereby inhibiting the proliferation of bovine preadipocytes.https://www.tandfonline.com/doi/10.1080/10495398.2024.2339406Beta-sitosterolbovine preadipocyteRNA-seqcell cycleCCNB1 |
| spellingShingle | Lei Jiang Yuan Wan Jinhai Pan Xiaoyu Mao Xiaolei Sun Linsen Zan Hongbao Wang Transcriptomic analysis reveals the inhibitory effect of beta-sitosterol on proliferation of bovine preadipocytes Animal Biotechnology Beta-sitosterol bovine preadipocyte RNA-seq cell cycle CCNB1 |
| title | Transcriptomic analysis reveals the inhibitory effect of beta-sitosterol on proliferation of bovine preadipocytes |
| title_full | Transcriptomic analysis reveals the inhibitory effect of beta-sitosterol on proliferation of bovine preadipocytes |
| title_fullStr | Transcriptomic analysis reveals the inhibitory effect of beta-sitosterol on proliferation of bovine preadipocytes |
| title_full_unstemmed | Transcriptomic analysis reveals the inhibitory effect of beta-sitosterol on proliferation of bovine preadipocytes |
| title_short | Transcriptomic analysis reveals the inhibitory effect of beta-sitosterol on proliferation of bovine preadipocytes |
| title_sort | transcriptomic analysis reveals the inhibitory effect of beta sitosterol on proliferation of bovine preadipocytes |
| topic | Beta-sitosterol bovine preadipocyte RNA-seq cell cycle CCNB1 |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2024.2339406 |
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