A large, general and modular DARPin–apoferritin scaffold enables the visualization of small proteins by cryo-EM

A large, general and modular DARPin–apoferritin scaffold enables the visualization of small proteins by cryo-EM

Single-particle cryo-electron microscopy (cryo-EM) has emerged as an indispensable technique in structural biology that is pivotal for deciphering protein architectures. However, the medium-sized proteins (30–40 kDa) that are prevalent in both eukaryotic and prokaryotic organisms often elude the res...

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Bibliographic Details
Main Authors: Xin Lu, Ming Yan, Yang Cai, Xi Song, Huan Chen, Mengtan Du, Zhenyi Wang, Jia'an Li, Liwen Niu, Fuxing Zeng, Quan Hao, Hongmin Zhang
Format: Article
Language:English
Published: International Union of Crystallography 2025-05-01
Series:IUCrJ
Subjects:
cryo-em
small proteins
scaffolds
darpin–apoferritin
Online Access:https://journals.iucr.org/paper?S2052252525003021
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