Activating cryptic biosynthetic gene clusters via ACTIMOT
The mainstream strategy of genome mining relies on the homologous activation and heterologous expression of target biosynthetic gene clusters (BGCs). However, the efficiency of the current techniques available for new compound discovery hardly complements these efforts. In a recent publication in Sc...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Engineering Microbiology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667370325000025 |
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| _version_ | 1849391123529728000 |
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| author | Xiaoying Bian |
| author_facet | Xiaoying Bian |
| author_sort | Xiaoying Bian |
| collection | DOAJ |
| description | The mainstream strategy of genome mining relies on the homologous activation and heterologous expression of target biosynthetic gene clusters (BGCs). However, the efficiency of the current techniques available for new compound discovery hardly complements these efforts. In a recent publication in Science, Xie et al. reported their breakthrough progress in expediting the discovery of untapped chemical diversity from bacteria by establishing the leveraged know-how of ACTIMOT (Advanced Cas9-mediaTed In vivo MObilization and mulTiplication of BGCs), offering a new avenue to access the unexploited, and even unpredictable, biosynthetic potential of bacteria. |
| format | Article |
| id | doaj-art-a4664856c69a49ca9857ef3c2019bb3a |
| institution | Kabale University |
| issn | 2667-3703 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Engineering Microbiology |
| spelling | doaj-art-a4664856c69a49ca9857ef3c2019bb3a2025-08-20T03:41:10ZengElsevierEngineering Microbiology2667-37032025-03-015110019010.1016/j.engmic.2025.100190Activating cryptic biosynthetic gene clusters via ACTIMOTXiaoying Bian0State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, Shandong, ChinaThe mainstream strategy of genome mining relies on the homologous activation and heterologous expression of target biosynthetic gene clusters (BGCs). However, the efficiency of the current techniques available for new compound discovery hardly complements these efforts. In a recent publication in Science, Xie et al. reported their breakthrough progress in expediting the discovery of untapped chemical diversity from bacteria by establishing the leveraged know-how of ACTIMOT (Advanced Cas9-mediaTed In vivo MObilization and mulTiplication of BGCs), offering a new avenue to access the unexploited, and even unpredictable, biosynthetic potential of bacteria.http://www.sciencedirect.com/science/article/pii/S2667370325000025Genome miningStreptomycesNatural productsACTIMOTCRISPR-Cas9 |
| spellingShingle | Xiaoying Bian Activating cryptic biosynthetic gene clusters via ACTIMOT Engineering Microbiology Genome mining Streptomyces Natural products ACTIMOT CRISPR-Cas9 |
| title | Activating cryptic biosynthetic gene clusters via ACTIMOT |
| title_full | Activating cryptic biosynthetic gene clusters via ACTIMOT |
| title_fullStr | Activating cryptic biosynthetic gene clusters via ACTIMOT |
| title_full_unstemmed | Activating cryptic biosynthetic gene clusters via ACTIMOT |
| title_short | Activating cryptic biosynthetic gene clusters via ACTIMOT |
| title_sort | activating cryptic biosynthetic gene clusters via actimot |
| topic | Genome mining Streptomyces Natural products ACTIMOT CRISPR-Cas9 |
| url | http://www.sciencedirect.com/science/article/pii/S2667370325000025 |
| work_keys_str_mv | AT xiaoyingbian activatingcrypticbiosyntheticgeneclustersviaactimot |