Multiphoton Microscopy for Ophthalmic Imaging
We review multiphoton microscopy (MPM) including two-photon autofluorescence (2PAF), second harmonic generation (SHG), third harmonic generation (THG), fluorescence lifetime (FLIM), and coherent anti-Stokes Raman Scattering (CARS) with relevance to clinical applications in ophthalmology. The differ...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2011/870879 |
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| _version_ | 1850171082687905792 |
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| author | Emily A. Gibson Omid Masihzadeh Tim C. Lei David A. Ammar Malik Y. Kahook |
| author_facet | Emily A. Gibson Omid Masihzadeh Tim C. Lei David A. Ammar Malik Y. Kahook |
| author_sort | Emily A. Gibson |
| collection | DOAJ |
| description | We review multiphoton microscopy (MPM) including two-photon autofluorescence (2PAF), second harmonic generation (SHG), third harmonic generation (THG), fluorescence lifetime (FLIM), and coherent anti-Stokes Raman Scattering (CARS) with relevance to clinical applications in ophthalmology. The different imaging modalities are discussed highlighting the particular strength that each has for functional tissue imaging. MPM is compared with current clinical ophthalmological imaging techniques such as reflectance confocal microscopy, optical coherence tomography, and fluorescence imaging. In addition, we discuss the future prospects for MPM in disease detection and clinical monitoring of disease progression, understanding fundamental disease mechanisms, and real-time monitoring of drug delivery. |
| format | Article |
| id | doaj-art-a462b806794e4e009cb43cc8ed8097f4 |
| institution | OA Journals |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-a462b806794e4e009cb43cc8ed8097f42025-08-20T02:20:20ZengWileyJournal of Ophthalmology2090-004X2090-00582011-01-01201110.1155/2011/870879870879Multiphoton Microscopy for Ophthalmic ImagingEmily A. Gibson0Omid Masihzadeh1Tim C. Lei2David A. Ammar3Malik Y. Kahook4Department of Bioengineering, University of Colorado Denver, Denver, 12700 E. 19th Ave, Mail Stop 8607, Aurora, CO 80045, USADepartment of Electrical Engineering, University of Colorado Denver, Denver, CO 80217, USADepartment of Electrical Engineering, University of Colorado Denver, Denver, CO 80217, USADepartment of Ophthalmology, School of Medicine, University of Colorado, 1675 Aurora Ct, Mail Stop F-731, Aurora, CO 80045, USADepartment of Ophthalmology, School of Medicine, University of Colorado, 1675 Aurora Ct, Mail Stop F-731, Aurora, CO 80045, USAWe review multiphoton microscopy (MPM) including two-photon autofluorescence (2PAF), second harmonic generation (SHG), third harmonic generation (THG), fluorescence lifetime (FLIM), and coherent anti-Stokes Raman Scattering (CARS) with relevance to clinical applications in ophthalmology. The different imaging modalities are discussed highlighting the particular strength that each has for functional tissue imaging. MPM is compared with current clinical ophthalmological imaging techniques such as reflectance confocal microscopy, optical coherence tomography, and fluorescence imaging. In addition, we discuss the future prospects for MPM in disease detection and clinical monitoring of disease progression, understanding fundamental disease mechanisms, and real-time monitoring of drug delivery.http://dx.doi.org/10.1155/2011/870879 |
| spellingShingle | Emily A. Gibson Omid Masihzadeh Tim C. Lei David A. Ammar Malik Y. Kahook Multiphoton Microscopy for Ophthalmic Imaging Journal of Ophthalmology |
| title | Multiphoton Microscopy for Ophthalmic Imaging |
| title_full | Multiphoton Microscopy for Ophthalmic Imaging |
| title_fullStr | Multiphoton Microscopy for Ophthalmic Imaging |
| title_full_unstemmed | Multiphoton Microscopy for Ophthalmic Imaging |
| title_short | Multiphoton Microscopy for Ophthalmic Imaging |
| title_sort | multiphoton microscopy for ophthalmic imaging |
| url | http://dx.doi.org/10.1155/2011/870879 |
| work_keys_str_mv | AT emilyagibson multiphotonmicroscopyforophthalmicimaging AT omidmasihzadeh multiphotonmicroscopyforophthalmicimaging AT timclei multiphotonmicroscopyforophthalmicimaging AT davidaammar multiphotonmicroscopyforophthalmicimaging AT malikykahook multiphotonmicroscopyforophthalmicimaging |