General Method for HPLC Purification and Sequencing of Selected dsDNA Gene Fragments from Complex PCRs Generated during Gene Expression Profiling
Gene expression profiling using an AFLP-based technique generates a large number of gene fragments that require identification by sequencing. The DNA fragments vary in length from about 50–500 bp. Ion-pair reversed-phase HPLC can be used to purify selected double-stranded DNA fragments that represen...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2000-04-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/00284rr07 |
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