General Method for HPLC Purification and Sequencing of Selected dsDNA Gene Fragments from Complex PCRs Generated during Gene Expression Profiling
Gene expression profiling using an AFLP-based technique generates a large number of gene fragments that require identification by sequencing. The DNA fragments vary in length from about 50–500 bp. Ion-pair reversed-phase HPLC can be used to purify selected double-stranded DNA fragments that represen...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2000-04-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/00284rr07 |
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| _version_ | 1850152774476496896 |
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| author | L.Y. Wong V. Belonogoff V.L. Boyd N.M. Hunkapiller P.M. Casey S.-N. Liew K.D. Lazaruk S. Baumhueter |
| author_facet | L.Y. Wong V. Belonogoff V.L. Boyd N.M. Hunkapiller P.M. Casey S.-N. Liew K.D. Lazaruk S. Baumhueter |
| author_sort | L.Y. Wong |
| collection | DOAJ |
| description | Gene expression profiling using an AFLP-based technique generates a large number of gene fragments that require identification by sequencing. The DNA fragments vary in length from about 50–500 bp. Ion-pair reversed-phase HPLC can be used to purify selected double-stranded DNA fragments that represent differentially expressed genes. The gene fragments are sequenced directly after vacuum drying of the collected HPLC fractions. |
| format | Article |
| id | doaj-art-a453f7e63db74282a0f70588554d0d5f |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2000-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-a453f7e63db74282a0f70588554d0d5f2025-08-20T02:25:53ZengTaylor & Francis GroupBioTechniques0736-62051940-98182000-04-0128477678310.2144/00284rr07General Method for HPLC Purification and Sequencing of Selected dsDNA Gene Fragments from Complex PCRs Generated during Gene Expression ProfilingL.Y. Wong0V. Belonogoff1V.L. Boyd2N.M. Hunkapiller3P.M. Casey4S.-N. Liew5K.D. Lazaruk6S. Baumhueter71Celera Genomics, Foster City, CA, USA1Celera Genomics, Foster City, CA, USA1Celera Genomics, Foster City, CA, USA1Celera Genomics, Foster City, CA, USA1Celera Genomics, Foster City, CA, USA1Celera Genomics, Foster City, CA, USA1Celera Genomics, Foster City, CA, USA1Celera Genomics, Foster City, CA, USAGene expression profiling using an AFLP-based technique generates a large number of gene fragments that require identification by sequencing. The DNA fragments vary in length from about 50–500 bp. Ion-pair reversed-phase HPLC can be used to purify selected double-stranded DNA fragments that represent differentially expressed genes. The gene fragments are sequenced directly after vacuum drying of the collected HPLC fractions.https://www.future-science.com/doi/10.2144/00284rr07 |
| spellingShingle | L.Y. Wong V. Belonogoff V.L. Boyd N.M. Hunkapiller P.M. Casey S.-N. Liew K.D. Lazaruk S. Baumhueter General Method for HPLC Purification and Sequencing of Selected dsDNA Gene Fragments from Complex PCRs Generated during Gene Expression Profiling BioTechniques |
| title | General Method for HPLC Purification and Sequencing of Selected dsDNA Gene Fragments from Complex PCRs Generated during Gene Expression Profiling |
| title_full | General Method for HPLC Purification and Sequencing of Selected dsDNA Gene Fragments from Complex PCRs Generated during Gene Expression Profiling |
| title_fullStr | General Method for HPLC Purification and Sequencing of Selected dsDNA Gene Fragments from Complex PCRs Generated during Gene Expression Profiling |
| title_full_unstemmed | General Method for HPLC Purification and Sequencing of Selected dsDNA Gene Fragments from Complex PCRs Generated during Gene Expression Profiling |
| title_short | General Method for HPLC Purification and Sequencing of Selected dsDNA Gene Fragments from Complex PCRs Generated during Gene Expression Profiling |
| title_sort | general method for hplc purification and sequencing of selected dsdna gene fragments from complex pcrs generated during gene expression profiling |
| url | https://www.future-science.com/doi/10.2144/00284rr07 |
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