Green-chemistry of by-products from Sai-Nam-Phueng orange in Thailand (1): Recovering a valuable source of bioactive compounds using response surface methodology, antioxidant, and cytotoxicity

Citrus reticulata Blanco, specifically Sai-Nam-Phueng orange traditionally considered by-products, are rich in phytochemicals — limonoids, flavonoids. This study compared seeds and peels for the phytochemical content and antioxidant activity. Using single-factor experiments and response surface meth...

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Main Authors: Kanchanok Kodchakorn, Prachya Kongtawelert
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Waste Management Bulletin
Online Access:http://www.sciencedirect.com/science/article/pii/S2949750724000889
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author Kanchanok Kodchakorn
Prachya Kongtawelert
author_facet Kanchanok Kodchakorn
Prachya Kongtawelert
author_sort Kanchanok Kodchakorn
collection DOAJ
description Citrus reticulata Blanco, specifically Sai-Nam-Phueng orange traditionally considered by-products, are rich in phytochemicals — limonoids, flavonoids. This study compared seeds and peels for the phytochemical content and antioxidant activity. Using single-factor experiments and response surface methodology (RSM), the effects of ethanol concentration, solvent-to-solid ratio, and temperature on extract yield were optimized. The study found optimal conditions to be 70.50 % ethanol, 28.11 mL/g solvent-to-solid ratio, and 69.99 °C. Experimental values are in reasonable agreement with predicted values. Phytochemical contents were characterized using HPLC, mass spectrometry, and NMR spectroscopy, successfully demonstrating efficient of purified compounds from orange by-products and cost-effective extraction methods.Kewords: Antioxidant, Green-extractions, Phytochemicals, Response Surface Methodology, Cytotoxicity.
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issn 2949-7507
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record_format Article
series Waste Management Bulletin
spelling doaj-art-a43909fa2d8943cca21aa1dcc91332ef2025-08-20T02:49:02ZengElsevierWaste Management Bulletin2949-75072024-12-012410812210.1016/j.wmb.2024.10.004Green-chemistry of by-products from Sai-Nam-Phueng orange in Thailand (1): Recovering a valuable source of bioactive compounds using response surface methodology, antioxidant, and cytotoxicityKanchanok Kodchakorn0Prachya Kongtawelert1Office of Research Administration, Chiang Mai University 50200, Thailand; Corresponding authors.Department of Biochemistry, Faculty of Medicine, Chiang Mai University 50200, Thailand; Corresponding authors.Citrus reticulata Blanco, specifically Sai-Nam-Phueng orange traditionally considered by-products, are rich in phytochemicals — limonoids, flavonoids. This study compared seeds and peels for the phytochemical content and antioxidant activity. Using single-factor experiments and response surface methodology (RSM), the effects of ethanol concentration, solvent-to-solid ratio, and temperature on extract yield were optimized. The study found optimal conditions to be 70.50 % ethanol, 28.11 mL/g solvent-to-solid ratio, and 69.99 °C. Experimental values are in reasonable agreement with predicted values. Phytochemical contents were characterized using HPLC, mass spectrometry, and NMR spectroscopy, successfully demonstrating efficient of purified compounds from orange by-products and cost-effective extraction methods.Kewords: Antioxidant, Green-extractions, Phytochemicals, Response Surface Methodology, Cytotoxicity.http://www.sciencedirect.com/science/article/pii/S2949750724000889
spellingShingle Kanchanok Kodchakorn
Prachya Kongtawelert
Green-chemistry of by-products from Sai-Nam-Phueng orange in Thailand (1): Recovering a valuable source of bioactive compounds using response surface methodology, antioxidant, and cytotoxicity
Waste Management Bulletin
title Green-chemistry of by-products from Sai-Nam-Phueng orange in Thailand (1): Recovering a valuable source of bioactive compounds using response surface methodology, antioxidant, and cytotoxicity
title_full Green-chemistry of by-products from Sai-Nam-Phueng orange in Thailand (1): Recovering a valuable source of bioactive compounds using response surface methodology, antioxidant, and cytotoxicity
title_fullStr Green-chemistry of by-products from Sai-Nam-Phueng orange in Thailand (1): Recovering a valuable source of bioactive compounds using response surface methodology, antioxidant, and cytotoxicity
title_full_unstemmed Green-chemistry of by-products from Sai-Nam-Phueng orange in Thailand (1): Recovering a valuable source of bioactive compounds using response surface methodology, antioxidant, and cytotoxicity
title_short Green-chemistry of by-products from Sai-Nam-Phueng orange in Thailand (1): Recovering a valuable source of bioactive compounds using response surface methodology, antioxidant, and cytotoxicity
title_sort green chemistry of by products from sai nam phueng orange in thailand 1 recovering a valuable source of bioactive compounds using response surface methodology antioxidant and cytotoxicity
url http://www.sciencedirect.com/science/article/pii/S2949750724000889
work_keys_str_mv AT kanchanokkodchakorn greenchemistryofbyproductsfromsainamphuengorangeinthailand1recoveringavaluablesourceofbioactivecompoundsusingresponsesurfacemethodologyantioxidantandcytotoxicity
AT prachyakongtawelert greenchemistryofbyproductsfromsainamphuengorangeinthailand1recoveringavaluablesourceofbioactivecompoundsusingresponsesurfacemethodologyantioxidantandcytotoxicity