Alternative Ways to Obtain Human Mesenchymal Stem Cells from Embryonic Stem Cells
Differentiation approaches to obtain mesenchymal stem cells (MSCs) have gradually developed over the last few decades. The problem is that different protocols give different MSC types, making further research difficult. Here, we tried three different approaches to differentiate embryonic stem cells...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-09-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/13/19/1617 |
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| author | Nikita Onyanov Olga Glazova Nawar Sakr Tatyana Krokunova Julia Krupinova Pavel Volchkov |
| author_facet | Nikita Onyanov Olga Glazova Nawar Sakr Tatyana Krokunova Julia Krupinova Pavel Volchkov |
| author_sort | Nikita Onyanov |
| collection | DOAJ |
| description | Differentiation approaches to obtain mesenchymal stem cells (MSCs) have gradually developed over the last few decades. The problem is that different protocols give different MSC types, making further research difficult. Here, we tried three different approaches to differentiate embryonic stem cells (ESCs) from early mesoderm to MSCs using serum-containing or xeno-free differentiation medium and observed differences in the cells’ morphology, doubling rate, ability to form colonies, surface marker analysis, and multilineage differentiation potential of the obtained cell lines. We concluded that the xeno-free medium best fits the criteria of MSCs’ morphology, growth kinetics, and surface marker characterization. In contrast, the serum-containing medium gives better potential for further MSC differentiation into osteogenic, chondrogenic, and adipogenic lineages. |
| format | Article |
| id | doaj-art-a42e2c9d51af496daa9eb9d9d271578c |
| institution | OA Journals |
| issn | 2073-4409 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj-art-a42e2c9d51af496daa9eb9d9d271578c2025-08-20T01:47:42ZengMDPI AGCells2073-44092024-09-011319161710.3390/cells13191617Alternative Ways to Obtain Human Mesenchymal Stem Cells from Embryonic Stem CellsNikita Onyanov0Olga Glazova1Nawar Sakr2Tatyana Krokunova3Julia Krupinova4Pavel Volchkov5Moscow Center for Advanced Studies, Kulakova Str. 20, Moscow 123592, RussiaFederal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, Moscow 125315, RussiaFederal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, Moscow 125315, RussiaMoscow Center for Advanced Studies, Kulakova Str. 20, Moscow 123592, RussiaFederal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, Moscow 125315, RussiaFederal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, Moscow 125315, RussiaDifferentiation approaches to obtain mesenchymal stem cells (MSCs) have gradually developed over the last few decades. The problem is that different protocols give different MSC types, making further research difficult. Here, we tried three different approaches to differentiate embryonic stem cells (ESCs) from early mesoderm to MSCs using serum-containing or xeno-free differentiation medium and observed differences in the cells’ morphology, doubling rate, ability to form colonies, surface marker analysis, and multilineage differentiation potential of the obtained cell lines. We concluded that the xeno-free medium best fits the criteria of MSCs’ morphology, growth kinetics, and surface marker characterization. In contrast, the serum-containing medium gives better potential for further MSC differentiation into osteogenic, chondrogenic, and adipogenic lineages.https://www.mdpi.com/2073-4409/13/19/1617stem celldifferentiationmesodermMSCs |
| spellingShingle | Nikita Onyanov Olga Glazova Nawar Sakr Tatyana Krokunova Julia Krupinova Pavel Volchkov Alternative Ways to Obtain Human Mesenchymal Stem Cells from Embryonic Stem Cells Cells stem cell differentiation mesoderm MSCs |
| title | Alternative Ways to Obtain Human Mesenchymal Stem Cells from Embryonic Stem Cells |
| title_full | Alternative Ways to Obtain Human Mesenchymal Stem Cells from Embryonic Stem Cells |
| title_fullStr | Alternative Ways to Obtain Human Mesenchymal Stem Cells from Embryonic Stem Cells |
| title_full_unstemmed | Alternative Ways to Obtain Human Mesenchymal Stem Cells from Embryonic Stem Cells |
| title_short | Alternative Ways to Obtain Human Mesenchymal Stem Cells from Embryonic Stem Cells |
| title_sort | alternative ways to obtain human mesenchymal stem cells from embryonic stem cells |
| topic | stem cell differentiation mesoderm MSCs |
| url | https://www.mdpi.com/2073-4409/13/19/1617 |
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