Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells
Brain organoids have been considered as an advanced platform for in vitro disease modeling and drug screening, but numerous roadblocks exist, such as lack of large-scale production technology and lengthy protocols with multiple manipulation steps, impeding the industrial translation of brain organoi...
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| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2023/3320211 |
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| author | Xuerui Yao Ji Hyun Kang Kee-Pyo Kim Hyogeun Shin Zhe-Long Jin Hao Guo Yong-Nan Xu Ying-Hua Li Sai Hali Jeongwoo Kwon Hyeonwoo La Chanhyeok Park Yong-June Kim Lin Wang Kwonho Hong Qilong Cao Il-Joo Cho Nam-Hyung Kim Dong Wook Han |
| author_facet | Xuerui Yao Ji Hyun Kang Kee-Pyo Kim Hyogeun Shin Zhe-Long Jin Hao Guo Yong-Nan Xu Ying-Hua Li Sai Hali Jeongwoo Kwon Hyeonwoo La Chanhyeok Park Yong-June Kim Lin Wang Kwonho Hong Qilong Cao Il-Joo Cho Nam-Hyung Kim Dong Wook Han |
| author_sort | Xuerui Yao |
| collection | DOAJ |
| description | Brain organoids have been considered as an advanced platform for in vitro disease modeling and drug screening, but numerous roadblocks exist, such as lack of large-scale production technology and lengthy protocols with multiple manipulation steps, impeding the industrial translation of brain organoid technology. Here, we describe the high-speed and large-scale production of midbrain organoids using a high-throughput screening-compatible platform within 30 days. Micro midbrain organoids (µMOs) exhibit a highly uniform morphology and gene expression pattern with minimal variability. Notably, µMOs show dramatically accelerated maturation, resulting in the generation of functional µMOs within only 30 days of differentiation. Furthermore, individual µMOs display highly consistent responsiveness to neurotoxin, suggesting their usefulness as an in vitro high-throughput drug toxicity screening platform. Collectively, our data indicate that µMO technology could represent an advanced and robust platform for in vitro disease modeling and drug screening for human neuronal diseases. |
| format | Article |
| id | doaj-art-a429f20de04345d09ff67eca862e03f6 |
| institution | OA Journals |
| issn | 1687-9678 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a429f20de04345d09ff67eca862e03f62025-08-20T02:20:21ZengWileyStem Cells International1687-96782023-01-01202310.1155/2023/3320211Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem CellsXuerui Yao0Ji Hyun Kang1Kee-Pyo Kim2Hyogeun Shin3Zhe-Long Jin4Hao Guo5Yong-Nan Xu6Ying-Hua Li7Sai Hali8Jeongwoo Kwon9Hyeonwoo La10Chanhyeok Park11Yong-June Kim12Lin Wang13Kwonho Hong14Qilong Cao15Il-Joo Cho16Nam-Hyung Kim17Dong Wook Han18Guangdong Provincial Key Laboratory of Large Animal Models for BiomedicineLaboratory of Stem Cells and OrganoidsDepartment of Life SciencesCenter for BioMicrosystemsGuangdong Provincial Key Laboratory of Large Animal Models for BiomedicineGuangdong Provincial Key Laboratory of Large Animal Models for BiomedicineGuangdong Provincial Key Laboratory of Large Animal Models for BiomedicineGuangdong Provincial Key Laboratory of Large Animal Models for BiomedicineInstitute of OphthalmologyPrimate Resources CenterDepartment of Stem Cell and Regenerative BiotechnologyDepartment of Stem Cell and Regenerative BiotechnologyDepartment of UrologyResearch and Development DepartmentDepartment of Stem Cell and Regenerative BiotechnologyResearch and Development DepartmentCenter for BioMicrosystemsGuangdong Provincial Key Laboratory of Large Animal Models for BiomedicineGuangdong Provincial Key Laboratory of Large Animal Models for BiomedicineBrain organoids have been considered as an advanced platform for in vitro disease modeling and drug screening, but numerous roadblocks exist, such as lack of large-scale production technology and lengthy protocols with multiple manipulation steps, impeding the industrial translation of brain organoid technology. Here, we describe the high-speed and large-scale production of midbrain organoids using a high-throughput screening-compatible platform within 30 days. Micro midbrain organoids (µMOs) exhibit a highly uniform morphology and gene expression pattern with minimal variability. Notably, µMOs show dramatically accelerated maturation, resulting in the generation of functional µMOs within only 30 days of differentiation. Furthermore, individual µMOs display highly consistent responsiveness to neurotoxin, suggesting their usefulness as an in vitro high-throughput drug toxicity screening platform. Collectively, our data indicate that µMO technology could represent an advanced and robust platform for in vitro disease modeling and drug screening for human neuronal diseases.http://dx.doi.org/10.1155/2023/3320211 |
| spellingShingle | Xuerui Yao Ji Hyun Kang Kee-Pyo Kim Hyogeun Shin Zhe-Long Jin Hao Guo Yong-Nan Xu Ying-Hua Li Sai Hali Jeongwoo Kwon Hyeonwoo La Chanhyeok Park Yong-June Kim Lin Wang Kwonho Hong Qilong Cao Il-Joo Cho Nam-Hyung Kim Dong Wook Han Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells Stem Cells International |
| title | Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells |
| title_full | Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells |
| title_fullStr | Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells |
| title_full_unstemmed | Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells |
| title_short | Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells |
| title_sort | production of highly uniform midbrain organoids from human pluripotent stem cells |
| url | http://dx.doi.org/10.1155/2023/3320211 |
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