Deciphering the dynamics and trophic mode distribution of the leaf spot-associated fungal community of eggplant (Solanum melongena L.)
Abstract The invasion of phytopathogens impacts the composition and associations of the internal microbial inhabitants. Leaf spot is one of the most devastating diseases in eggplant var. Mattu Gulla which is unique in terms of geographic indication (GI) status. Leaf spot samples (asymptomatic and sy...
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BMC
2024-11-01
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| Series: | Phytopathology Research |
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| Online Access: | https://doi.org/10.1186/s42483-024-00277-2 |
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| author | Arya Kaniyassery Sudhanva Bhimanakatte Sathish Sachin Ashok Thorat Thokur Sreepathy Murali Mattu Radhakrishna Rao Annamalai Muthusamy |
| author_facet | Arya Kaniyassery Sudhanva Bhimanakatte Sathish Sachin Ashok Thorat Thokur Sreepathy Murali Mattu Radhakrishna Rao Annamalai Muthusamy |
| author_sort | Arya Kaniyassery |
| collection | DOAJ |
| description | Abstract The invasion of phytopathogens impacts the composition and associations of the internal microbial inhabitants. Leaf spot is one of the most devastating diseases in eggplant var. Mattu Gulla which is unique in terms of geographic indication (GI) status. Leaf spot samples (asymptomatic and symptomatic) were collected to characterize the fungal community associated with them using culture-based and next-generation ITS rRNA-based metabarcoding approaches. Both methods showed that Ascomycota and Basidiomycota were the predominant phyla in both groups. In the asymptomatic group, Didymosphaeriaceae, Pleosporaceae, Trichomeriaceae, and Capnodiaceae were the most differentially abundant families. In contrast, Phaeosphaeriaceae, Pleosporaceae, Didymellaceae, Rhynchogastremataceae, and Bulleribasidiaceae were the most differentially abundant families in the symptomatic group. At the genus level, Cladosporium was the most differentially abundant genus in the asymptomatic group. In the symptomatic group Alternaria, Remotididymella, Vishniacozyma, Bulleribasidium, Occultifur, Epicoccum, and Loratospora were the abundant genera. The pathotroph-saprotrophic mode was the most common mode identified in both groups, with an increased abundance in the symptomatic group. Seven fungal families and two genera were identified as common according to the culture-based method and NGS analysis based on ITS rRNA metabarcoding. Our study indicated that the composition of the core microbial community varies with plant health status, and a combination of culturable and next-generation ITS rRNA-based metabarcoding approaches could be a reliable option for obtaining a detailed understanding of plant-associated fungal communities. |
| format | Article |
| id | doaj-art-a4279b82cc9543debef7d6c97a25ddbf |
| institution | OA Journals |
| issn | 2524-4167 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
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| series | Phytopathology Research |
| spelling | doaj-art-a4279b82cc9543debef7d6c97a25ddbf2025-08-20T02:13:31ZengBMCPhytopathology Research2524-41672024-11-016111810.1186/s42483-024-00277-2Deciphering the dynamics and trophic mode distribution of the leaf spot-associated fungal community of eggplant (Solanum melongena L.)Arya Kaniyassery0Sudhanva Bhimanakatte Sathish1Sachin Ashok Thorat2Thokur Sreepathy Murali3Mattu Radhakrishna Rao4Annamalai Muthusamy5Department of Plant Sciences, Manipal School of Life Sciences, Manipal Academy of Higher Education (MAHE)Department of Plant Sciences, Manipal School of Life Sciences, Manipal Academy of Higher Education (MAHE)Department of Plant Sciences, Manipal School of Life Sciences, Manipal Academy of Higher Education (MAHE)Department of Public Health Genomics, Manipal School of Life Sciences, Manipal Academy of Higher Education (MAHE)PragathiDepartment of Plant Sciences, Manipal School of Life Sciences, Manipal Academy of Higher Education (MAHE)Abstract The invasion of phytopathogens impacts the composition and associations of the internal microbial inhabitants. Leaf spot is one of the most devastating diseases in eggplant var. Mattu Gulla which is unique in terms of geographic indication (GI) status. Leaf spot samples (asymptomatic and symptomatic) were collected to characterize the fungal community associated with them using culture-based and next-generation ITS rRNA-based metabarcoding approaches. Both methods showed that Ascomycota and Basidiomycota were the predominant phyla in both groups. In the asymptomatic group, Didymosphaeriaceae, Pleosporaceae, Trichomeriaceae, and Capnodiaceae were the most differentially abundant families. In contrast, Phaeosphaeriaceae, Pleosporaceae, Didymellaceae, Rhynchogastremataceae, and Bulleribasidiaceae were the most differentially abundant families in the symptomatic group. At the genus level, Cladosporium was the most differentially abundant genus in the asymptomatic group. In the symptomatic group Alternaria, Remotididymella, Vishniacozyma, Bulleribasidium, Occultifur, Epicoccum, and Loratospora were the abundant genera. The pathotroph-saprotrophic mode was the most common mode identified in both groups, with an increased abundance in the symptomatic group. Seven fungal families and two genera were identified as common according to the culture-based method and NGS analysis based on ITS rRNA metabarcoding. Our study indicated that the composition of the core microbial community varies with plant health status, and a combination of culturable and next-generation ITS rRNA-based metabarcoding approaches could be a reliable option for obtaining a detailed understanding of plant-associated fungal communities.https://doi.org/10.1186/s42483-024-00277-2EggplantLeaf spotITSMetabarcodingFungal communityTrophic mode |
| spellingShingle | Arya Kaniyassery Sudhanva Bhimanakatte Sathish Sachin Ashok Thorat Thokur Sreepathy Murali Mattu Radhakrishna Rao Annamalai Muthusamy Deciphering the dynamics and trophic mode distribution of the leaf spot-associated fungal community of eggplant (Solanum melongena L.) Phytopathology Research Eggplant Leaf spot ITS Metabarcoding Fungal community Trophic mode |
| title | Deciphering the dynamics and trophic mode distribution of the leaf spot-associated fungal community of eggplant (Solanum melongena L.) |
| title_full | Deciphering the dynamics and trophic mode distribution of the leaf spot-associated fungal community of eggplant (Solanum melongena L.) |
| title_fullStr | Deciphering the dynamics and trophic mode distribution of the leaf spot-associated fungal community of eggplant (Solanum melongena L.) |
| title_full_unstemmed | Deciphering the dynamics and trophic mode distribution of the leaf spot-associated fungal community of eggplant (Solanum melongena L.) |
| title_short | Deciphering the dynamics and trophic mode distribution of the leaf spot-associated fungal community of eggplant (Solanum melongena L.) |
| title_sort | deciphering the dynamics and trophic mode distribution of the leaf spot associated fungal community of eggplant solanum melongena l |
| topic | Eggplant Leaf spot ITS Metabarcoding Fungal community Trophic mode |
| url | https://doi.org/10.1186/s42483-024-00277-2 |
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