Analysis of Amelogenin and Sex-determining Region on Y Chromosome Genes Obtained from Pulpal Tissue for Sex Estimation by using Multiplex Polymerase Chain Reaction
In forensic odontology, Amelogenin and Sex-Determining Region on Y chromosome genes are commonly used for sex identification. Nevertheless, the amplification of both of these genes encounters limitations in determining sex, primarily due to deletions observed in certain racial groups and the influe...
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| Format: | Article |
| Language: | English |
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Sulaimani Polytechnic University
2023-12-01
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| Series: | Kurdistan Journal of Applied Research |
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| Online Access: | https://kjar.spu.edu.iq/index.php/kjar/article/view/948 |
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| author | Mohammed Taha Ahmed Baban Dena Nadhim Mohammad Natheer Al-Rawi |
| author_facet | Mohammed Taha Ahmed Baban Dena Nadhim Mohammad Natheer Al-Rawi |
| author_sort | Mohammed Taha Ahmed Baban |
| collection | DOAJ |
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In forensic odontology, Amelogenin and Sex-Determining Region on Y chromosome genes are commonly used for sex identification. Nevertheless, the amplification of both of these genes encounters limitations in determining sex, primarily due to deletions observed in certain racial groups and the influence of genetic disorders on these genes. This research aims to assess the precision of simultaneously amplifying both genes using multiplex polymerase chain reaction on samples derived from teeth that have been subjected to various forensic conditions. In this study, a total of 70 teeth, comprising 35 males and 35 females, were utilized, and all samples underwent genetic analysis to determine sex following exposure to different forensic scenarios. The findings revealed that both the quantity and quality of genetic material were reduced when exposed to harsh environmental conditions. Notably, the amplification of the sex-determining region on the Y chromosome gene outperformed the amplification of Amelogenin and achieved a sex-determination accuracy rate of 100%. In conclusion, dental pulp serves as a valuable source of genetic material even after exposure to diverse environmental conditions, and it can effectively be used for sex estimation purposes.
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| format | Article |
| id | doaj-art-a425e038b98e402a9be0b1dde0df6cb1 |
| institution | Kabale University |
| issn | 2411-7684 2411-7706 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Sulaimani Polytechnic University |
| record_format | Article |
| series | Kurdistan Journal of Applied Research |
| spelling | doaj-art-a425e038b98e402a9be0b1dde0df6cb12025-02-09T20:59:35ZengSulaimani Polytechnic UniversityKurdistan Journal of Applied Research2411-76842411-77062023-12-018210.24017/science.2023.2.8Analysis of Amelogenin and Sex-determining Region on Y Chromosome Genes Obtained from Pulpal Tissue for Sex Estimation by using Multiplex Polymerase Chain ReactionMohammed Taha Ahmed Baban0https://orcid.org/0000-0003-2559-8427Dena Nadhim Mohammad 1Natheer Al-Rawi 2https://orcid.org/0000-0002-7483-6594Department of Dental Nursing, Sulaimani Technical Institute, Sulaimani Polytechnic University, Sulaymaniyah, IraqDepartment of Oral Diagnosis, College of Dentistry, University of Sulaimani, Sulaymaniyah, IraqDepartmentof Oral &Maxillofacial Pathology, University of Sharjah, Sharjah, United Arab Emirates In forensic odontology, Amelogenin and Sex-Determining Region on Y chromosome genes are commonly used for sex identification. Nevertheless, the amplification of both of these genes encounters limitations in determining sex, primarily due to deletions observed in certain racial groups and the influence of genetic disorders on these genes. This research aims to assess the precision of simultaneously amplifying both genes using multiplex polymerase chain reaction on samples derived from teeth that have been subjected to various forensic conditions. In this study, a total of 70 teeth, comprising 35 males and 35 females, were utilized, and all samples underwent genetic analysis to determine sex following exposure to different forensic scenarios. The findings revealed that both the quantity and quality of genetic material were reduced when exposed to harsh environmental conditions. Notably, the amplification of the sex-determining region on the Y chromosome gene outperformed the amplification of Amelogenin and achieved a sex-determination accuracy rate of 100%. In conclusion, dental pulp serves as a valuable source of genetic material even after exposure to diverse environmental conditions, and it can effectively be used for sex estimation purposes. https://kjar.spu.edu.iq/index.php/kjar/article/view/948Sex determinationAmelogeninSRYMultiplex PCRForensic odontology |
| spellingShingle | Mohammed Taha Ahmed Baban Dena Nadhim Mohammad Natheer Al-Rawi Analysis of Amelogenin and Sex-determining Region on Y Chromosome Genes Obtained from Pulpal Tissue for Sex Estimation by using Multiplex Polymerase Chain Reaction Kurdistan Journal of Applied Research Sex determination Amelogenin SRY Multiplex PCR Forensic odontology |
| title | Analysis of Amelogenin and Sex-determining Region on Y Chromosome Genes Obtained from Pulpal Tissue for Sex Estimation by using Multiplex Polymerase Chain Reaction |
| title_full | Analysis of Amelogenin and Sex-determining Region on Y Chromosome Genes Obtained from Pulpal Tissue for Sex Estimation by using Multiplex Polymerase Chain Reaction |
| title_fullStr | Analysis of Amelogenin and Sex-determining Region on Y Chromosome Genes Obtained from Pulpal Tissue for Sex Estimation by using Multiplex Polymerase Chain Reaction |
| title_full_unstemmed | Analysis of Amelogenin and Sex-determining Region on Y Chromosome Genes Obtained from Pulpal Tissue for Sex Estimation by using Multiplex Polymerase Chain Reaction |
| title_short | Analysis of Amelogenin and Sex-determining Region on Y Chromosome Genes Obtained from Pulpal Tissue for Sex Estimation by using Multiplex Polymerase Chain Reaction |
| title_sort | analysis of amelogenin and sex determining region on y chromosome genes obtained from pulpal tissue for sex estimation by using multiplex polymerase chain reaction |
| topic | Sex determination Amelogenin SRY Multiplex PCR Forensic odontology |
| url | https://kjar.spu.edu.iq/index.php/kjar/article/view/948 |
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