Mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line NCI-H292
Objective To investigate the effects of 20% mannitol injection on the proliferation and apoptosis of human lung squamous carcinoma cell line NCI-H292 and its possible mechanisms. Methods NCI-H292 cells were incubated with 20% and lower concentrations of mannitol. Cell proliferation and viability wer...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2025-03-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-3-281-336.pdf |
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| _version_ | 1850275842238709760 |
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| author | LI Huangyi, HUANG Daohang, ZHANG Wenyu, HUANG Dingming, HUANG Qichun |
| author_facet | LI Huangyi, HUANG Daohang, ZHANG Wenyu, HUANG Dingming, HUANG Qichun |
| author_sort | LI Huangyi, HUANG Daohang, ZHANG Wenyu, HUANG Dingming, HUANG Qichun |
| collection | DOAJ |
| description | Objective To investigate the effects of 20% mannitol injection on the proliferation and apoptosis of human lung squamous carcinoma cell line NCI-H292 and its possible mechanisms. Methods NCI-H292 cells were incubated with 20% and lower concentrations of mannitol. Cell proliferation and viability were detected by CCK-8 assay. Cell apoptosis was detected by flow cytometry and the expression of mitochondrial apoptosis pathway-related proteins Bcl-2 and Bax was detected by Western blot. Results Compared with the control group, mannitol inhibited cell proliferation in a dose- and time-dependent manner (P＜ 0.05), 20% mannitol could induce cell apoptosis (P＜ 0.05). The expression level of Bcl-2 was significantly reduced, while that expression level of Bax was significantly increased in cells incubated with 20% mannitol (P＜ 0.05). Conclusions Clinically used 20% mannitol injection can induce apoptosis of lung squamous carcinoma cell line NCI-H292. Its mechanism is related to mitochondrial pathway-related proteins Bcl-2 and Bax. |
| format | Article |
| id | doaj-art-a4237c1dfa144935b7e4677ea958be14 |
| institution | OA Journals |
| issn | 1001-6325 |
| language | zho |
| publishDate | 2025-03-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj-art-a4237c1dfa144935b7e4677ea958be142025-08-20T01:50:33ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252025-03-0145333634010.16352/j.issn.1001-6325.2025.03.0336Mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line NCI-H292LI Huangyi, HUANG Daohang, ZHANG Wenyu, HUANG Dingming, HUANG Qichun0Department of Pharmacy, Guangxi Medical University Affiliated Cancer Hospital, Nanning 530021, ChinaObjective To investigate the effects of 20% mannitol injection on the proliferation and apoptosis of human lung squamous carcinoma cell line NCI-H292 and its possible mechanisms. Methods NCI-H292 cells were incubated with 20% and lower concentrations of mannitol. Cell proliferation and viability were detected by CCK-8 assay. Cell apoptosis was detected by flow cytometry and the expression of mitochondrial apoptosis pathway-related proteins Bcl-2 and Bax was detected by Western blot. Results Compared with the control group, mannitol inhibited cell proliferation in a dose- and time-dependent manner (P＜ 0.05), 20% mannitol could induce cell apoptosis (P＜ 0.05). The expression level of Bcl-2 was significantly reduced, while that expression level of Bax was significantly increased in cells incubated with 20% mannitol (P＜ 0.05). Conclusions Clinically used 20% mannitol injection can induce apoptosis of lung squamous carcinoma cell line NCI-H292. Its mechanism is related to mitochondrial pathway-related proteins Bcl-2 and Bax.https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-3-281-336.pdfmannitol|lung squamous cell carcinoma|apoptosis|bcl-2|bax |
| spellingShingle | LI Huangyi, HUANG Daohang, ZHANG Wenyu, HUANG Dingming, HUANG Qichun Mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line NCI-H292 Jichu yixue yu linchuang mannitol|lung squamous cell carcinoma|apoptosis|bcl-2|bax |
| title | Mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line NCI-H292 |
| title_full | Mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line NCI-H292 |
| title_fullStr | Mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line NCI-H292 |
| title_full_unstemmed | Mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line NCI-H292 |
| title_short | Mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line NCI-H292 |
| title_sort | mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line nci h292 |
| topic | mannitol|lung squamous cell carcinoma|apoptosis|bcl-2|bax |
| url | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-3-281-336.pdf |
| work_keys_str_mv | AT lihuangyihuangdaohangzhangwenyuhuangdingminghuangqichun mannitolpromotestheapoptosisofhumanlungsquamouscarcinomacelllinencih292 |