Saponins enhance the stability and cost-efficiency of human embryonic stem cell culture
Abstract The cultivation and differentiation of human embryonic stem cells (hESCs) into organoids are crucial for advancing of new drug development and personalized cell therapies. Despite establishing of chemically defined hESC culture media over the past decade, these media's reliance on grow...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-01-01
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| Series: | Cell Regeneration |
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| Online Access: | https://doi.org/10.1186/s13619-024-00220-y |
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| author | Jingyi Shi Mei Wu Shi Fang Zhuo Liu Huihui Liu Ying Zhao Linlin Liu Zhicheng Shao |
| author_facet | Jingyi Shi Mei Wu Shi Fang Zhuo Liu Huihui Liu Ying Zhao Linlin Liu Zhicheng Shao |
| author_sort | Jingyi Shi |
| collection | DOAJ |
| description | Abstract The cultivation and differentiation of human embryonic stem cells (hESCs) into organoids are crucial for advancing of new drug development and personalized cell therapies. Despite establishing of chemically defined hESC culture media over the past decade, these media's reliance on growth factors, which are costly and prone to degradation, poses a challenge for sustained and stable cell culture. Here, we introduce an hESC culture system(E6Bs) that facilitates the long-term, genetically stable expansion of hESCs, enabling cells to consistently sustain high levels of pluripotency markers, including NANOG, SOX2, TRA-1–60, and SSEA4, across extended periods. Moreover, organoids derived from hESCs using this medium were successfully established and expanded for at least one month, exhibiting differentiation into cortical organoids, GABAergic precursor organoids and heart-forming organoids. This innovative system offers a robust tool for preserving hESC homeostasis and modeling the nervous system in vitro. |
| format | Article |
| id | doaj-art-a4157a70615c44c2ab2004cfc8459f78 |
| institution | DOAJ |
| issn | 2045-9769 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Cell Regeneration |
| spelling | doaj-art-a4157a70615c44c2ab2004cfc8459f782025-08-20T03:06:00ZengSpringerOpenCell Regeneration2045-97692025-01-0114111310.1186/s13619-024-00220-ySaponins enhance the stability and cost-efficiency of human embryonic stem cell cultureJingyi Shi0Mei Wu1Shi Fang2Zhuo Liu3Huihui Liu4Ying Zhao5Linlin Liu6Zhicheng Shao7Department of Neurology, Zhongshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan UniversityDepartment of Neurology, Zhongshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan UniversityDepartment of Neurology, Zhongshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan UniversityDepartment of Neurology, Zhongshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan UniversityDepartment of Neurology, Zhongshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan UniversitySchool of Pharmacy, Xinxiang Medical UniversityDepartment of Neurology, Zhongshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan UniversityDepartment of Neurology, Zhongshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan UniversityAbstract The cultivation and differentiation of human embryonic stem cells (hESCs) into organoids are crucial for advancing of new drug development and personalized cell therapies. Despite establishing of chemically defined hESC culture media over the past decade, these media's reliance on growth factors, which are costly and prone to degradation, poses a challenge for sustained and stable cell culture. Here, we introduce an hESC culture system(E6Bs) that facilitates the long-term, genetically stable expansion of hESCs, enabling cells to consistently sustain high levels of pluripotency markers, including NANOG, SOX2, TRA-1–60, and SSEA4, across extended periods. Moreover, organoids derived from hESCs using this medium were successfully established and expanded for at least one month, exhibiting differentiation into cortical organoids, GABAergic precursor organoids and heart-forming organoids. This innovative system offers a robust tool for preserving hESC homeostasis and modeling the nervous system in vitro.https://doi.org/10.1186/s13619-024-00220-ySaponinsHuman embryonic stem cellsCulture mediumBrain organoid |
| spellingShingle | Jingyi Shi Mei Wu Shi Fang Zhuo Liu Huihui Liu Ying Zhao Linlin Liu Zhicheng Shao Saponins enhance the stability and cost-efficiency of human embryonic stem cell culture Cell Regeneration Saponins Human embryonic stem cells Culture medium Brain organoid |
| title | Saponins enhance the stability and cost-efficiency of human embryonic stem cell culture |
| title_full | Saponins enhance the stability and cost-efficiency of human embryonic stem cell culture |
| title_fullStr | Saponins enhance the stability and cost-efficiency of human embryonic stem cell culture |
| title_full_unstemmed | Saponins enhance the stability and cost-efficiency of human embryonic stem cell culture |
| title_short | Saponins enhance the stability and cost-efficiency of human embryonic stem cell culture |
| title_sort | saponins enhance the stability and cost efficiency of human embryonic stem cell culture |
| topic | Saponins Human embryonic stem cells Culture medium Brain organoid |
| url | https://doi.org/10.1186/s13619-024-00220-y |
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