Isolation, Screening and Antibiotic Sensitivity of Pseudomonas species from Kelana Jaya Lake Soil in Selangor Malaysia
Pathogenic microorganisms from hospitals, communities, and the environment remain great threats to human health. The increasing concern about antibiotic resistance has also necessitated the search for robust alternatives. Therefore, this study aims to isolate, screen and evaluate the antibiotic susc...
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| Format: | Article |
| Language: | English |
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University of Baghdad, College of Science for Women
2021-09-01
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| Series: | مجلة بغداد للعلوم |
| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/4728 |
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| author | Idris A. Ahmed Abdalati I Aljondi Alabed Ali A. Alabed Abdullah Y. Al-Mahdi Rasheed Abdsalam |
| author_facet | Idris A. Ahmed Abdalati I Aljondi Alabed Ali A. Alabed Abdullah Y. Al-Mahdi Rasheed Abdsalam |
| author_sort | Idris A. Ahmed |
| collection | DOAJ |
| description | Pathogenic microorganisms from hospitals, communities, and the environment remain great threats to human health. The increasing concern about antibiotic resistance has also necessitated the search for robust alternatives. Therefore, this study aims to isolate, screen and evaluate the antibiotic susceptibility of Pseudomonas aeruginosa isolated from a soil sample taken from northern, western and eastern parts of Kelana Jaya Lake against four antibiotics (gentamycin, tetracycline, ampicillin, and penicillin) on a Mueller-Hinton Agar media plate. Pseudomonas identification was done by using API 20 kit. Disc diffusion was employed as well as the oxidase test. From the positive oxidase result, the isolated bacteria were identified as Burkholderia cepacia (97.6% ID), Pseudomonas aeruginosa (99.5-99.9% ID), and Pseudomonas fluorescent (75.9% ID). Only Pseudomonas aeruginosa isolates were further evaluated for antibiotic susceptibility tests. The result showed that P. aeruginosa was susceptible to only three antibiotics (gentamycin, tetracycline, and penicillin) showing a clear zone of inhibition while it was resistant to only ampicillin with no zone of inhibition. Soil isolates are potential sources for the development of effective antibiotics against resistant bacteria.
Keywords: Antibiotics; Bacterial resistance; Sensitivity, Pseudomonas |
| format | Article |
| id | doaj-art-a410457cedc541558dff3c4a1e7f9d4e |
| institution | Kabale University |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-a410457cedc541558dff3c4a1e7f9d4e2025-08-20T03:35:48ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862021-09-0118310.21123/bsj.2021.18.3.0455Isolation, Screening and Antibiotic Sensitivity of Pseudomonas species from Kelana Jaya Lake Soil in Selangor MalaysiaIdris A. Ahmed0Abdalati I Aljondi1Alabed Ali A. Alabed2Abdullah Y. Al-Mahdi3Rasheed Abdsalam4Biotechnology Department, Faculty of Science, Lincoln University College, 47301, Petaling Jaya, Selangor, Malaysia.Biotechnology Department, Faculty of Science, Lincoln University College, 47301, Petaling Jaya, Selangor, Malaysia.Community Medicine Department, Faculty of Medicine, Lincoln University College, 47301 Petaling Jaya, Selangor, Malaysia.Microbiology Department, Faculty of Medicine Lincoln University College, 47301, Petaling Jaya, Selangor, Malaysia.Faculty of Dentistry, Lincoln University College, 47301, Petaling Jaya, Selangor, Malaysia.Pathogenic microorganisms from hospitals, communities, and the environment remain great threats to human health. The increasing concern about antibiotic resistance has also necessitated the search for robust alternatives. Therefore, this study aims to isolate, screen and evaluate the antibiotic susceptibility of Pseudomonas aeruginosa isolated from a soil sample taken from northern, western and eastern parts of Kelana Jaya Lake against four antibiotics (gentamycin, tetracycline, ampicillin, and penicillin) on a Mueller-Hinton Agar media plate. Pseudomonas identification was done by using API 20 kit. Disc diffusion was employed as well as the oxidase test. From the positive oxidase result, the isolated bacteria were identified as Burkholderia cepacia (97.6% ID), Pseudomonas aeruginosa (99.5-99.9% ID), and Pseudomonas fluorescent (75.9% ID). Only Pseudomonas aeruginosa isolates were further evaluated for antibiotic susceptibility tests. The result showed that P. aeruginosa was susceptible to only three antibiotics (gentamycin, tetracycline, and penicillin) showing a clear zone of inhibition while it was resistant to only ampicillin with no zone of inhibition. Soil isolates are potential sources for the development of effective antibiotics against resistant bacteria. Keywords: Antibiotics; Bacterial resistance; Sensitivity, Pseudomonashttps://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/4728 |
| spellingShingle | Idris A. Ahmed Abdalati I Aljondi Alabed Ali A. Alabed Abdullah Y. Al-Mahdi Rasheed Abdsalam Isolation, Screening and Antibiotic Sensitivity of Pseudomonas species from Kelana Jaya Lake Soil in Selangor Malaysia مجلة بغداد للعلوم |
| title | Isolation, Screening and Antibiotic Sensitivity of Pseudomonas species from Kelana Jaya Lake Soil in Selangor Malaysia |
| title_full | Isolation, Screening and Antibiotic Sensitivity of Pseudomonas species from Kelana Jaya Lake Soil in Selangor Malaysia |
| title_fullStr | Isolation, Screening and Antibiotic Sensitivity of Pseudomonas species from Kelana Jaya Lake Soil in Selangor Malaysia |
| title_full_unstemmed | Isolation, Screening and Antibiotic Sensitivity of Pseudomonas species from Kelana Jaya Lake Soil in Selangor Malaysia |
| title_short | Isolation, Screening and Antibiotic Sensitivity of Pseudomonas species from Kelana Jaya Lake Soil in Selangor Malaysia |
| title_sort | isolation screening and antibiotic sensitivity of pseudomonas species from kelana jaya lake soil in selangor malaysia |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/4728 |
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