Evaluation of the level of 5'-nucleotidase and some biochemical parameters in the sera of beta-thalassemia patients
One of the hydrolytic enzymes that catalyzes the hydrolysis of 5' -nucleotides into ribonucleosides and phosphate is the 5'nucleotidase, which is found in several organs. Oxidative stress shows depletion of antioxidants, such as glutathione (GSH), and the increased concentration of produc...
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Tikrit University
2023-12-01
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| Series: | Tikrit Journal of Pure Science |
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| Online Access: | https://tjpsj.org/index.php/tjps/article/view/1331 |
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| author | Sameer M. Al-Gorany Mohammed Rajeh Altutnchi |
| author_facet | Sameer M. Al-Gorany Mohammed Rajeh Altutnchi |
| author_sort | Sameer M. Al-Gorany |
| collection | DOAJ |
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One of the hydrolytic enzymes that catalyzes the hydrolysis of 5' -nucleotides into ribonucleosides and phosphate is the 5'nucleotidase, which is found in several organs. Oxidative stress shows depletion of antioxidants, such as glutathione (GSH), and the increased concentration of product of lipid peroxidation, such as MDA. The study comprised a total of 54 subjects including patients with beta thalassemia (n=27) and healthy volunteers (n=27) matched by age and gender. Hematology (Hb and % PCV), 5'-nucleotidase, Ferritin, GSH and MDA were measured using different methods and devices. The 5'nucleotidase, Ferritin, and MDA levels were significantly increased (p<0.001) in beta-thalassemia patients compared to the controls. As opposed to that, the Hb, % PCV and GSH levels were significantly decreased (p<0.001) in patients with beta-thalassemia. There was a negative significant correlation (r = -0.146, -0.245, p<0.05) between Hb and 5'- NT enzyme and PCV% with 5'- NT enzyme, respectively. While Hb had a high significant correlation (r = 0.956, p<0.05) with PCV%. In addition, 5'- NT enzyme had a positive significant correlation with GSH, MDA and Ferritin (r = 0.584, 0.442, 0.735, p<0.05), respectively. The present findings indicated an increase in the level of effectiveness of the 5'nucleotidase enzyme, ferritin and GSH in β thalassemia patients while decreasing in MDA.
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| format | Article |
| id | doaj-art-a40d355e7cfb48a9bed6671f0246c1f3 |
| institution | Kabale University |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Tikrit University |
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| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-a40d355e7cfb48a9bed6671f0246c1f32025-08-20T03:28:59ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262023-12-0128610.25130/tjps.v28i6.1331Evaluation of the level of 5'-nucleotidase and some biochemical parameters in the sera of beta-thalassemia patientsSameer M. Al-Gorany0Mohammed Rajeh Altutnchi1Department of Medical Laboratory Techniques, Baaquba Technical Institute, Middle Technical University, Baghdad, IraqTechnical Engineering College, Northern Technical University, Mosul, Iraq One of the hydrolytic enzymes that catalyzes the hydrolysis of 5' -nucleotides into ribonucleosides and phosphate is the 5'nucleotidase, which is found in several organs. Oxidative stress shows depletion of antioxidants, such as glutathione (GSH), and the increased concentration of product of lipid peroxidation, such as MDA. The study comprised a total of 54 subjects including patients with beta thalassemia (n=27) and healthy volunteers (n=27) matched by age and gender. Hematology (Hb and % PCV), 5'-nucleotidase, Ferritin, GSH and MDA were measured using different methods and devices. The 5'nucleotidase, Ferritin, and MDA levels were significantly increased (p<0.001) in beta-thalassemia patients compared to the controls. As opposed to that, the Hb, % PCV and GSH levels were significantly decreased (p<0.001) in patients with beta-thalassemia. There was a negative significant correlation (r = -0.146, -0.245, p<0.05) between Hb and 5'- NT enzyme and PCV% with 5'- NT enzyme, respectively. While Hb had a high significant correlation (r = 0.956, p<0.05) with PCV%. In addition, 5'- NT enzyme had a positive significant correlation with GSH, MDA and Ferritin (r = 0.584, 0.442, 0.735, p<0.05), respectively. The present findings indicated an increase in the level of effectiveness of the 5'nucleotidase enzyme, ferritin and GSH in β thalassemia patients while decreasing in MDA. https://tjpsj.org/index.php/tjps/article/view/13315’‑NucleotidaseOxidative stressGlutathioneFerritinBeta- thalassemia |
| spellingShingle | Sameer M. Al-Gorany Mohammed Rajeh Altutnchi Evaluation of the level of 5'-nucleotidase and some biochemical parameters in the sera of beta-thalassemia patients Tikrit Journal of Pure Science 5’‑Nucleotidase Oxidative stress Glutathione Ferritin Beta- thalassemia |
| title | Evaluation of the level of 5'-nucleotidase and some biochemical parameters in the sera of beta-thalassemia patients |
| title_full | Evaluation of the level of 5'-nucleotidase and some biochemical parameters in the sera of beta-thalassemia patients |
| title_fullStr | Evaluation of the level of 5'-nucleotidase and some biochemical parameters in the sera of beta-thalassemia patients |
| title_full_unstemmed | Evaluation of the level of 5'-nucleotidase and some biochemical parameters in the sera of beta-thalassemia patients |
| title_short | Evaluation of the level of 5'-nucleotidase and some biochemical parameters in the sera of beta-thalassemia patients |
| title_sort | evaluation of the level of 5 nucleotidase and some biochemical parameters in the sera of beta thalassemia patients |
| topic | 5’‑Nucleotidase Oxidative stress Glutathione Ferritin Beta- thalassemia |
| url | https://tjpsj.org/index.php/tjps/article/view/1331 |
| work_keys_str_mv | AT sameermalgorany evaluationofthelevelof5nucleotidaseandsomebiochemicalparametersintheseraofbetathalassemiapatients AT mohammedrajehaltutnchi evaluationofthelevelof5nucleotidaseandsomebiochemicalparametersintheseraofbetathalassemiapatients |