Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris.

Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris.

We have investigated the relationship between the stability and secreted yield of a series of mutational variants of human lysozyme (HuL) in Pichia pastoris. We show that genes directly involved in the unfolded protein response (UPR), ER-associated degradation (ERAD) and ER-phagy are transcriptional...

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Main Authors: Graham Whyteside, Marcos J C Alcocer, Janet R Kumita, Christopher M Dobson, Maria Lazarou, Richard J Pleass, David B Archer
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0022692&type=printable
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