Purity determination on silkworm hybrid rate using RAPD technique
It was found that specifity of DNA fingerprinting of the genome from individual silkworm can be characterized on three different levels, namly, species-specific, variety-specific and individual-specific level. Only pecularity of DNA that are varietie-specific can serve as juge criterion for purity d...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2003-05-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.03.0321 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849708456030765056 |
|---|---|
| author | ZHONG Bo-xiong ZHANG Jin-wei DING Nong TAO jing-ya LU Yun-chong |
| author_facet | ZHONG Bo-xiong ZHANG Jin-wei DING Nong TAO jing-ya LU Yun-chong |
| author_sort | ZHONG Bo-xiong |
| collection | DOAJ |
| description | It was found that specifity of DNA fingerprinting of the genome from individual silkworm can be characterized on three different levels, namly, species-specific, variety-specific and individual-specific level. Only pecularity of DNA that are varietie-specific can serve as juge criterion for purity determination on the hybrid. The same PCR result can be amplified by DNA template from a single silkworm egg or a newly hatched larva and from silkworm gland cells. So fingerprint based on DNA at the egg stage is promising to be used in rapidly assessing purity of hybrid. |
| format | Article |
| id | doaj-art-a4043fd6c75b4afbbe4a16acdd18e02a |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2003-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-a4043fd6c75b4afbbe4a16acdd18e02a2025-08-20T03:15:38ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552003-05-012932132410.3785/1008-9209.2003.03.032110089209Purity determination on silkworm hybrid rate using RAPD techniqueZHONG Bo-xiongZHANG Jin-weiDING NongTAO jing-yaLU Yun-chongIt was found that specifity of DNA fingerprinting of the genome from individual silkworm can be characterized on three different levels, namly, species-specific, variety-specific and individual-specific level. Only pecularity of DNA that are varietie-specific can serve as juge criterion for purity determination on the hybrid. The same PCR result can be amplified by DNA template from a single silkworm egg or a newly hatched larva and from silkworm gland cells. So fingerprint based on DNA at the egg stage is promising to be used in rapidly assessing purity of hybrid.https://www.academax.com/doi/10.3785/1008-9209.2003.03.0321silkworm <italic>Bombyx mori</italic> L.DNA fingerprintPCRhybrid rateegg |
| spellingShingle | ZHONG Bo-xiong ZHANG Jin-wei DING Nong TAO jing-ya LU Yun-chong Purity determination on silkworm hybrid rate using RAPD technique 浙江大学学报. 农业与生命科学版 silkworm <italic>Bombyx mori</italic> L. DNA fingerprint PCR hybrid rate egg |
| title | Purity determination on silkworm hybrid rate using RAPD technique |
| title_full | Purity determination on silkworm hybrid rate using RAPD technique |
| title_fullStr | Purity determination on silkworm hybrid rate using RAPD technique |
| title_full_unstemmed | Purity determination on silkworm hybrid rate using RAPD technique |
| title_short | Purity determination on silkworm hybrid rate using RAPD technique |
| title_sort | purity determination on silkworm hybrid rate using rapd technique |
| topic | silkworm <italic>Bombyx mori</italic> L. DNA fingerprint PCR hybrid rate egg |
| url | https://www.academax.com/doi/10.3785/1008-9209.2003.03.0321 |
| work_keys_str_mv | AT zhongboxiong puritydeterminationonsilkwormhybridrateusingrapdtechnique AT zhangjinwei puritydeterminationonsilkwormhybridrateusingrapdtechnique AT dingnong puritydeterminationonsilkwormhybridrateusingrapdtechnique AT taojingya puritydeterminationonsilkwormhybridrateusingrapdtechnique AT luyunchong puritydeterminationonsilkwormhybridrateusingrapdtechnique |