In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medications

Aims: This study was carried out to identify and outline the degree of relationship between immunophenotyped macrophages expressing CD163 and PDGF-B in cyclosporine-A, phenytoin, and nifedipine-induced gingival overgrowth. Methods: Eighty adult male albino rats were selected and divided into four eq...

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Main Authors: Amina J. Almahrog, Lobna R.S. Radwan, Rehab R. El-Zehery, Mohamed I. Mourad, Mohammed E. Grawish
Format: Article
Language:English
Published: Elsevier 2016-01-01
Series:Journal of Oral Biology and Craniofacial Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2212426815001396
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author Amina J. Almahrog
Lobna R.S. Radwan
Rehab R. El-Zehery
Mohamed I. Mourad
Mohammed E. Grawish
author_facet Amina J. Almahrog
Lobna R.S. Radwan
Rehab R. El-Zehery
Mohamed I. Mourad
Mohammed E. Grawish
author_sort Amina J. Almahrog
collection DOAJ
description Aims: This study was carried out to identify and outline the degree of relationship between immunophenotyped macrophages expressing CD163 and PDGF-B in cyclosporine-A, phenytoin, and nifedipine-induced gingival overgrowth. Methods: Eighty adult male albino rats were selected and divided into four equal groups. Group I received no treatment. Rats of groups II, III, and IV were administered cyclosporine-A, phenytoin, and nifedipine, respectively. Routine tissue processing was carried out for staining with CD163 and PDGF-B. The results of this study were analyzed statistically. Results: Group I exhibited score 0 gingival overgrowth while group II yielded score 3 with blunt and bulbous gingival crests. Rats of group III showed score 2 with knife edge and group IV revealed less pronounced gingival overgrowth and mostly the gingival crest was knife edge. Group II had the highest mean value for CD163 while group I showed the lowest value. In addition, group II had the highest mean value for PDGF-B while group I showed the lowest value. Statistically, there was an overall significant difference between the studied groups as well as between each two groups. Conclusion: Strong association exists between immunophenotyped macrophages expressing CD163 and PDGF-B in GO induced by these medications. In addition, CD163 and PDGF-B upregulated in cyclosporine-A-induced GO compared to phenytoin and nifedipine medications.
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spelling doaj-art-a3fbe464aa144d8daa44456baf04e1242025-08-20T02:50:27ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682016-01-0161111810.1016/j.jobcr.2015.12.009In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medicationsAmina J. Almahrog0Lobna R.S. Radwan1Rehab R. El-Zehery2Mohamed I. Mourad3Mohammed E. Grawish4Department of Oral Biology, Faculty of Dentistry, Almergib University, LibyaDepartment of Oral Biology, Faculty of Dentistry, Mansoura University, EgyptDepartment of Oral Biology, Faculty of Dentistry, Mansoura University, EgyptDepartment of Oral Pathology, Faculty of Dentistry, Mansoura University, EgyptDepartment of Oral Biology, Faculty of Dentistry, Mansoura University, Egypt; Department of Oral Biology, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Gamasa, Mansoura, Egypt; Corresponding author.Aims: This study was carried out to identify and outline the degree of relationship between immunophenotyped macrophages expressing CD163 and PDGF-B in cyclosporine-A, phenytoin, and nifedipine-induced gingival overgrowth. Methods: Eighty adult male albino rats were selected and divided into four equal groups. Group I received no treatment. Rats of groups II, III, and IV were administered cyclosporine-A, phenytoin, and nifedipine, respectively. Routine tissue processing was carried out for staining with CD163 and PDGF-B. The results of this study were analyzed statistically. Results: Group I exhibited score 0 gingival overgrowth while group II yielded score 3 with blunt and bulbous gingival crests. Rats of group III showed score 2 with knife edge and group IV revealed less pronounced gingival overgrowth and mostly the gingival crest was knife edge. Group II had the highest mean value for CD163 while group I showed the lowest value. In addition, group II had the highest mean value for PDGF-B while group I showed the lowest value. Statistically, there was an overall significant difference between the studied groups as well as between each two groups. Conclusion: Strong association exists between immunophenotyped macrophages expressing CD163 and PDGF-B in GO induced by these medications. In addition, CD163 and PDGF-B upregulated in cyclosporine-A-induced GO compared to phenytoin and nifedipine medications.http://www.sciencedirect.com/science/article/pii/S2212426815001396Cyclosporine-APhenytoinNifedipineCD163PDGF-B
spellingShingle Amina J. Almahrog
Lobna R.S. Radwan
Rehab R. El-Zehery
Mohamed I. Mourad
Mohammed E. Grawish
In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medications
Journal of Oral Biology and Craniofacial Research
Cyclosporine-A
Phenytoin
Nifedipine
CD163
PDGF-B
title In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medications
title_full In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medications
title_fullStr In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medications
title_full_unstemmed In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medications
title_short In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medications
title_sort in vivo association of immunophenotyped macrophages expressing cd163 with pdgf b in gingival overgrowth induced by three different categories of medications
topic Cyclosporine-A
Phenytoin
Nifedipine
CD163
PDGF-B
url http://www.sciencedirect.com/science/article/pii/S2212426815001396
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