Oxygen-delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme B PET imaging in malignant tumors
Abstract Limited treatment response and inadequate monitoring methods stand firmly before successful immunotherapy. Recruiting and activating immune cells in the hypoxic tumor microenvironment is the key to reversing immune suppression and improving immunotherapy efficacy. In this study, biomimetic...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-03-01
|
| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-025-03257-6 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850251602763448320 |
|---|---|
| author | Xingyi Wang Hanyi Fang Wenzhu Hu Yuan Feng Zhangyongxue Zhou Mengyan Hu Dawei Jiang Yongxue Zhang Xiaoli Lan |
| author_facet | Xingyi Wang Hanyi Fang Wenzhu Hu Yuan Feng Zhangyongxue Zhou Mengyan Hu Dawei Jiang Yongxue Zhang Xiaoli Lan |
| author_sort | Xingyi Wang |
| collection | DOAJ |
| description | Abstract Limited treatment response and inadequate monitoring methods stand firmly before successful immunotherapy. Recruiting and activating immune cells in the hypoxic tumor microenvironment is the key to reversing immune suppression and improving immunotherapy efficacy. In this study, biomimetic oxygen-delivering nanoparticles (CmPF) are engineered for homologous targeting and hypoxia alleviation within the tumor environment. CmPF targets the tumor microenvironment and delivers oxygen to reduce hypoxia, thereby promoting immune cell activity at the tumor site. In addition, granzyme B-targeted positron emission tomography (PET) imaging is employed to monitor immune cell activity changes in response to immunotherapy efficacy in vivo. The combination of CmPF with carboplatin and PD-1 inhibitors significantly suppresses tumor growth by 2.4-fold, exhibiting the potential of CmPF to enhance the efficacy of immunotherapy. Immunohistochemistry further confirms increased expression of key immune markers, highlighting the reprogramming of the tumor microenvironment. This study demonstrates that hypoxia alleviation enhances tumor immunotherapy efficacy and introduces a non-invasive PET imaging method for dynamic, real-time assessment of therapeutic response. |
| format | Article |
| id | doaj-art-a3f968d35ea94f668f21d5759bb720a7 |
| institution | OA Journals |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-a3f968d35ea94f668f21d5759bb720a72025-08-20T01:57:51ZengBMCJournal of Nanobiotechnology1477-31552025-03-0123111510.1186/s12951-025-03257-6Oxygen-delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme B PET imaging in malignant tumorsXingyi Wang0Hanyi Fang1Wenzhu Hu2Yuan Feng3Zhangyongxue Zhou4Mengyan Hu5Dawei Jiang6Yongxue Zhang7Xiaoli Lan8Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Limited treatment response and inadequate monitoring methods stand firmly before successful immunotherapy. Recruiting and activating immune cells in the hypoxic tumor microenvironment is the key to reversing immune suppression and improving immunotherapy efficacy. In this study, biomimetic oxygen-delivering nanoparticles (CmPF) are engineered for homologous targeting and hypoxia alleviation within the tumor environment. CmPF targets the tumor microenvironment and delivers oxygen to reduce hypoxia, thereby promoting immune cell activity at the tumor site. In addition, granzyme B-targeted positron emission tomography (PET) imaging is employed to monitor immune cell activity changes in response to immunotherapy efficacy in vivo. The combination of CmPF with carboplatin and PD-1 inhibitors significantly suppresses tumor growth by 2.4-fold, exhibiting the potential of CmPF to enhance the efficacy of immunotherapy. Immunohistochemistry further confirms increased expression of key immune markers, highlighting the reprogramming of the tumor microenvironment. This study demonstrates that hypoxia alleviation enhances tumor immunotherapy efficacy and introduces a non-invasive PET imaging method for dynamic, real-time assessment of therapeutic response.https://doi.org/10.1186/s12951-025-03257-6HypoxiaOxygen delivery nanoparticlesImmunotherapyGranzyme BPositron emission tomography imaging |
| spellingShingle | Xingyi Wang Hanyi Fang Wenzhu Hu Yuan Feng Zhangyongxue Zhou Mengyan Hu Dawei Jiang Yongxue Zhang Xiaoli Lan Oxygen-delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme B PET imaging in malignant tumors Journal of Nanobiotechnology Hypoxia Oxygen delivery nanoparticles Immunotherapy Granzyme B Positron emission tomography imaging |
| title | Oxygen-delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme B PET imaging in malignant tumors |
| title_full | Oxygen-delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme B PET imaging in malignant tumors |
| title_fullStr | Oxygen-delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme B PET imaging in malignant tumors |
| title_full_unstemmed | Oxygen-delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme B PET imaging in malignant tumors |
| title_short | Oxygen-delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme B PET imaging in malignant tumors |
| title_sort | oxygen delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme b pet imaging in malignant tumors |
| topic | Hypoxia Oxygen delivery nanoparticles Immunotherapy Granzyme B Positron emission tomography imaging |
| url | https://doi.org/10.1186/s12951-025-03257-6 |
| work_keys_str_mv | AT xingyiwang oxygendeliverynanoparticlesenhancedimmunotherapyefficacymonitoredbygranzymebpetimaginginmalignanttumors AT hanyifang oxygendeliverynanoparticlesenhancedimmunotherapyefficacymonitoredbygranzymebpetimaginginmalignanttumors AT wenzhuhu oxygendeliverynanoparticlesenhancedimmunotherapyefficacymonitoredbygranzymebpetimaginginmalignanttumors AT yuanfeng oxygendeliverynanoparticlesenhancedimmunotherapyefficacymonitoredbygranzymebpetimaginginmalignanttumors AT zhangyongxuezhou oxygendeliverynanoparticlesenhancedimmunotherapyefficacymonitoredbygranzymebpetimaginginmalignanttumors AT mengyanhu oxygendeliverynanoparticlesenhancedimmunotherapyefficacymonitoredbygranzymebpetimaginginmalignanttumors AT daweijiang oxygendeliverynanoparticlesenhancedimmunotherapyefficacymonitoredbygranzymebpetimaginginmalignanttumors AT yongxuezhang oxygendeliverynanoparticlesenhancedimmunotherapyefficacymonitoredbygranzymebpetimaginginmalignanttumors AT xiaolilan oxygendeliverynanoparticlesenhancedimmunotherapyefficacymonitoredbygranzymebpetimaginginmalignanttumors |