Time series analysis of ex-vivo ischemia-reperfused heart using Q-space imaging
Abstract In the evaluation of myocardial infarction, cardiac MRI remains problematic in terms of sensitivity and quantitativeness. Thus, we sought to delineate pathological microstructural alterations across temporal phases subsequent to myocardial ischemia–reperfusion (IR) injury utilizing q-space...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-14394-1 |
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| author | Genki Ichihara Junichi Hata Daisuke Nakashima Kyohei Daigo Kazuhisa Sugai Yuji Iwasawa Motoaki Sano Masaya Nakamura Hideyuki Okano Kazuki Sato Yoshinori Katsumata |
| author_facet | Genki Ichihara Junichi Hata Daisuke Nakashima Kyohei Daigo Kazuhisa Sugai Yuji Iwasawa Motoaki Sano Masaya Nakamura Hideyuki Okano Kazuki Sato Yoshinori Katsumata |
| author_sort | Genki Ichihara |
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| description | Abstract In the evaluation of myocardial infarction, cardiac MRI remains problematic in terms of sensitivity and quantitativeness. Thus, we sought to delineate pathological microstructural alterations across temporal phases subsequent to myocardial ischemia–reperfusion (IR) injury utilizing q-space imaging (QSI), an advanced diffusion imaging methodology proficient in assessing the non-Gaussian diffusion patterns of molecules. Rats were subjected to IR injury, and infarct was evaluated at 2, 7, and 30 days via histopathological staining and MRI using a 9.4‐Tesla system. QSI was performed with gadolinium-enhanced T1-weighted imaging (T1WI) for comparative assessment. Correlation coefficients among images were computed at each level of the left ventricular short-axis cross-section to juxtapose the infarct dimensions and morphology. Axial diffusivity of the kurtosis maps at 2, 7, and 30 days revealed substantial correlations with the hyperintense areas noted on T1WI (R2 = 0.885, 0.947, and 0.929, respectively). Furthermore, a strong correlation was noted with the transmural extent of infarction. Even in the absence of gadolinium contrast, the high-intensity regions delineated by QSI were concordant with the extent of pathological infarction (R2 = 0.977). These findings highlight the capability of QSI in identifying pathological changes at the infarct site, independent of gadolinium contrast media and irrespective of the temporal phase subsequent to the IR onset. |
| format | Article |
| id | doaj-art-a3f727adb7914e8aad29ca099ed7475e |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-a3f727adb7914e8aad29ca099ed7475e2025-08-20T03:42:29ZengNature PortfolioScientific Reports2045-23222025-08-0115111410.1038/s41598-025-14394-1Time series analysis of ex-vivo ischemia-reperfused heart using Q-space imagingGenki Ichihara0Junichi Hata1Daisuke Nakashima2Kyohei Daigo3Kazuhisa Sugai4Yuji Iwasawa5Motoaki Sano6Masaya Nakamura7Hideyuki Okano8Kazuki Sato9Yoshinori Katsumata10Department of Cardiology, Keio University School of MedicineGraduate School of Human Health Sciences, Tokyo Metropolitan UniversityDepartment of Orthopaedic Surgery, Keio University School of MedicineDepartment of Cardiology, Keio University School of MedicineDivision of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science UniversityInstitute for Integrated Sports Medicine, Keio University School of MedicineDepartment of Cardiology, Keio University School of MedicineDepartment of Orthopaedic Surgery, Keio University School of MedicineLaboratory for Marmoset Models of Brain Diseases, RIKEN Center for Brain ScienceInstitute for Integrated Sports Medicine, Keio University School of MedicineDepartment of Cardiology, Keio University School of MedicineAbstract In the evaluation of myocardial infarction, cardiac MRI remains problematic in terms of sensitivity and quantitativeness. Thus, we sought to delineate pathological microstructural alterations across temporal phases subsequent to myocardial ischemia–reperfusion (IR) injury utilizing q-space imaging (QSI), an advanced diffusion imaging methodology proficient in assessing the non-Gaussian diffusion patterns of molecules. Rats were subjected to IR injury, and infarct was evaluated at 2, 7, and 30 days via histopathological staining and MRI using a 9.4‐Tesla system. QSI was performed with gadolinium-enhanced T1-weighted imaging (T1WI) for comparative assessment. Correlation coefficients among images were computed at each level of the left ventricular short-axis cross-section to juxtapose the infarct dimensions and morphology. Axial diffusivity of the kurtosis maps at 2, 7, and 30 days revealed substantial correlations with the hyperintense areas noted on T1WI (R2 = 0.885, 0.947, and 0.929, respectively). Furthermore, a strong correlation was noted with the transmural extent of infarction. Even in the absence of gadolinium contrast, the high-intensity regions delineated by QSI were concordant with the extent of pathological infarction (R2 = 0.977). These findings highlight the capability of QSI in identifying pathological changes at the infarct site, independent of gadolinium contrast media and irrespective of the temporal phase subsequent to the IR onset.https://doi.org/10.1038/s41598-025-14394-1Diffusion-weighted imagingIschemia reperfusionMagnetic resonance imagingMyocardial infarctionq-Space imaging |
| spellingShingle | Genki Ichihara Junichi Hata Daisuke Nakashima Kyohei Daigo Kazuhisa Sugai Yuji Iwasawa Motoaki Sano Masaya Nakamura Hideyuki Okano Kazuki Sato Yoshinori Katsumata Time series analysis of ex-vivo ischemia-reperfused heart using Q-space imaging Scientific Reports Diffusion-weighted imaging Ischemia reperfusion Magnetic resonance imaging Myocardial infarction q-Space imaging |
| title | Time series analysis of ex-vivo ischemia-reperfused heart using Q-space imaging |
| title_full | Time series analysis of ex-vivo ischemia-reperfused heart using Q-space imaging |
| title_fullStr | Time series analysis of ex-vivo ischemia-reperfused heart using Q-space imaging |
| title_full_unstemmed | Time series analysis of ex-vivo ischemia-reperfused heart using Q-space imaging |
| title_short | Time series analysis of ex-vivo ischemia-reperfused heart using Q-space imaging |
| title_sort | time series analysis of ex vivo ischemia reperfused heart using q space imaging |
| topic | Diffusion-weighted imaging Ischemia reperfusion Magnetic resonance imaging Myocardial infarction q-Space imaging |
| url | https://doi.org/10.1038/s41598-025-14394-1 |
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