The amount of DNA and RNA in primary cancer cells and their metastases
Considering the significant impact of oncopathology on global morbidity and mortality, as well as changes in its prevalence and typology, research into the molecular-genetic mechanisms of oncogenesis and metastasis, particularly the role of nucleic acids, becomes crucial. The aim of this study is to...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2024-08-01
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| Series: | Healthcare in Low-resource Settings |
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| Online Access: | https://www.pagepressjournals.org/hls/article/view/12456 |
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| author | Oleksandr Herasymenko Kostyantyn Gerasymenko Olha Herasymenko |
| author_facet | Oleksandr Herasymenko Kostyantyn Gerasymenko Olha Herasymenko |
| author_sort | Oleksandr Herasymenko |
| collection | DOAJ |
| description | Considering the significant impact of oncopathology on global morbidity and mortality, as well as changes in its prevalence and typology, research into the molecular-genetic mechanisms of oncogenesis and metastasis, particularly the role of nucleic acids, becomes crucial. The aim of this study is to determine the content of DNA and RNA in cells of primary cancer and its metastases through microspectrophotometric analysis of malignant cells collected from 30 patients with primary and metastatic oncological processes. The following methods were employed: microspectrophotometric analysis using the automated complex “Protva-PM11-DVK-3m,” staining of histological specimens with halo cyanin-chrome alum, the discrete statistics method, frequency and cluster analysis, and the bibliographic method. Changes in the concentration of nucleic acids in the cells of primary and metastatic tumors were identified during the study. The changes did not always correlate with each other, indicating morphofunctional polymorphism in tumor cell populations. A trend towards a decrease in nuclear RNA concentration and an increase in cytoplasmic RNA concentration in metastases were observed, suggesting alterations in metabolic and synthetic processes in cells and a decrease in their differentiation degree.
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| format | Article |
| id | doaj-art-a3f4b394f58745d5ba8691ccac53e10a |
| institution | Kabale University |
| issn | 2281-7824 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Healthcare in Low-resource Settings |
| spelling | doaj-art-a3f4b394f58745d5ba8691ccac53e10a2025-02-01T10:56:17ZengPAGEPress PublicationsHealthcare in Low-resource Settings2281-78242024-08-0112s2The amount of DNA and RNA in primary cancer cells and their metastasesOleksandr Herasymenko0Kostyantyn Gerasymenko1Olha Herasymenko2Department of Pathological Anatomy, Forensic Medicine, Histology, Donetsk National Medical University, KropyvnytskyiDepartment of Pathological Anatomy, Forensic Medicine, Histology, Donetsk National Medical University, KropyvnytskyiFeofaniya Clinical Hospital, KyivConsidering the significant impact of oncopathology on global morbidity and mortality, as well as changes in its prevalence and typology, research into the molecular-genetic mechanisms of oncogenesis and metastasis, particularly the role of nucleic acids, becomes crucial. The aim of this study is to determine the content of DNA and RNA in cells of primary cancer and its metastases through microspectrophotometric analysis of malignant cells collected from 30 patients with primary and metastatic oncological processes. The following methods were employed: microspectrophotometric analysis using the automated complex “Protva-PM11-DVK-3m,” staining of histological specimens with halo cyanin-chrome alum, the discrete statistics method, frequency and cluster analysis, and the bibliographic method. Changes in the concentration of nucleic acids in the cells of primary and metastatic tumors were identified during the study. The changes did not always correlate with each other, indicating morphofunctional polymorphism in tumor cell populations. A trend towards a decrease in nuclear RNA concentration and an increase in cytoplasmic RNA concentration in metastases were observed, suggesting alterations in metabolic and synthetic processes in cells and a decrease in their differentiation degree. https://www.pagepressjournals.org/hls/article/view/12456Carcinogenesisadvanced molecular genetic analysismicrospectrophotometrynucleic acidsoncodiagnosisnon-cell autonomous effectors |
| spellingShingle | Oleksandr Herasymenko Kostyantyn Gerasymenko Olha Herasymenko The amount of DNA and RNA in primary cancer cells and their metastases Healthcare in Low-resource Settings Carcinogenesis advanced molecular genetic analysis microspectrophotometry nucleic acids oncodiagnosis non-cell autonomous effectors |
| title | The amount of DNA and RNA in primary cancer cells and their metastases |
| title_full | The amount of DNA and RNA in primary cancer cells and their metastases |
| title_fullStr | The amount of DNA and RNA in primary cancer cells and their metastases |
| title_full_unstemmed | The amount of DNA and RNA in primary cancer cells and their metastases |
| title_short | The amount of DNA and RNA in primary cancer cells and their metastases |
| title_sort | amount of dna and rna in primary cancer cells and their metastases |
| topic | Carcinogenesis advanced molecular genetic analysis microspectrophotometry nucleic acids oncodiagnosis non-cell autonomous effectors |
| url | https://www.pagepressjournals.org/hls/article/view/12456 |
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