Discordance between humoral and cellular immune responses to cytomegalovirus infection in CMV seropositive patients awaiting lung transplantation
IntroductionRisk stratification for CMV infection in lung transplantation (LT) currently relies on determining donor and recipient CMV IgG before transplantation. However, it has been observed that some patients who test positive for CMV-specific humoral response before kidney transplantation (KT) e...
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Frontiers Media S.A.
2025-01-01
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| author | Meritxell Boada-Pérez Meritxell Boada-Pérez Meritxell Boada-Pérez Cristina Berastegui Cristina Berastegui Cristina Berastegui Marta Erro Marta Erro Piedad Ussetti Elena Crespo Elena Crespo Laura Donadeu Laura Donadeu Oriol Bestard Oriol Bestard Oriol Bestard Gabriel Anguera Amparo Solé Ricardo Ponz Mir Brian Molloy Eva Revilla-López Eva Revilla-López Víctor Monforte Víctor Monforte Víctor Monforte Susana Gómez-Ollés Susana Gómez-Ollés Susana Gómez-Ollés |
| author_facet | Meritxell Boada-Pérez Meritxell Boada-Pérez Meritxell Boada-Pérez Cristina Berastegui Cristina Berastegui Cristina Berastegui Marta Erro Marta Erro Piedad Ussetti Elena Crespo Elena Crespo Laura Donadeu Laura Donadeu Oriol Bestard Oriol Bestard Oriol Bestard Gabriel Anguera Amparo Solé Ricardo Ponz Mir Brian Molloy Eva Revilla-López Eva Revilla-López Víctor Monforte Víctor Monforte Víctor Monforte Susana Gómez-Ollés Susana Gómez-Ollés Susana Gómez-Ollés |
| author_sort | Meritxell Boada-Pérez |
| collection | DOAJ |
| description | IntroductionRisk stratification for CMV infection in lung transplantation (LT) currently relies on determining donor and recipient CMV IgG before transplantation. However, it has been observed that some patients who test positive for CMV-specific humoral response before kidney transplantation (KT) exhibit a weak or absent CMV-specific cellular response. The significance of this observation in LT is still unknown.MethodsThis prospective, multicenter, observational study evaluated the agreement between CMV IgG serology and specific cell-mediated response (specific T cell Enzyme-Linked ImmunoSpot Assay, ELISPOT, against CMV pp65 and IE-1 antigens) in 121 patients on the waiting list for LT.ResultsOne hundred and four (86%) patients were seropositive for CMV. Discordant humoral and cellular immunologic responses were observed, 29% of seropositive patients had a weak ELISPOT response to IE-1 and 39% to pp65. In 22% of seropositive patients, there was a weak or no response to both antigens. All seronegative patients did not respond to either antigen.ConclusionsTherefore, over 20% of CMV seropositive LT candidates showed weak CMV-specific cellular immune responses despite detectable serological memory against CMV. This may be important in assessing the risk of developing a CMV infection after transplantation. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj-art-a3ebc9503a5c4b8ca4d9eb25d1e78ac52025-01-22T07:11:11ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-01-011510.3389/fimmu.2024.14455531445553Discordance between humoral and cellular immune responses to cytomegalovirus infection in CMV seropositive patients awaiting lung transplantationMeritxell Boada-Pérez0Meritxell Boada-Pérez1Meritxell Boada-Pérez2Cristina Berastegui3Cristina Berastegui4Cristina Berastegui5Marta Erro6Marta Erro7Piedad Ussetti8Elena Crespo9Elena Crespo10Laura Donadeu11Laura Donadeu12Oriol Bestard13Oriol Bestard14Oriol Bestard15Gabriel Anguera16Amparo Solé17Ricardo Ponz Mir18Brian Molloy19Eva Revilla-López20Eva Revilla-López21Víctor Monforte22Víctor Monforte23Víctor Monforte24Susana Gómez-Ollés25Susana Gómez-Ollés26Susana Gómez-Ollés27Department of Medicine, Universitat Autònoma de Barcelona, Barcelona, SpainDepartment of Pulmonology, Vall d’Hebron Research Institute, Barcelona, SpainVall d’Hebron for Solid Organ Transplantation Research Group, Vall d’Hebron Research Institute, Vall d’Hebron Barcelona Hospital, Universitat Autònoma de Barcelona, Barcelona, SpainVall d’Hebron for Solid Organ Transplantation Research Group, Vall d’Hebron Research Institute, Vall d’Hebron Barcelona Hospital, Universitat Autònoma de Barcelona, Barcelona, SpainLung Transplant Unit, Pulmonology Department, Vall d’Hebron University Hospital, Barcelona, SpainCentro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, SpainVall d’Hebron for Solid Organ Transplantation Research Group, Vall d’Hebron Research Institute, Vall d’Hebron Barcelona Hospital, Universitat Autònoma de Barcelona, Barcelona, SpainDepartment of Pulmonary Medicine, Puerta de Hierro Majadahonda University Hospital, Majadahonda, Madrid, SpainDepartment of Pulmonary Medicine, Puerta de Hierro Majadahonda University Hospital, Majadahonda, Madrid, SpainVall d’Hebron for Solid Organ Transplantation Research Group, Vall d’Hebron Research Institute, Vall d’Hebron Barcelona Hospital, Universitat Autònoma de Barcelona, Barcelona, SpainLaboratory of Nephrology and Transplantation, Vall d’Hebron Research Institute, Vall d’Hebron Barcelona Hospital, Universitat Autònoma de Barcelona, Barcelona, SpainVall d’Hebron for Solid Organ Transplantation Research Group, Vall d’Hebron Research Institute, Vall d’Hebron Barcelona Hospital, Universitat Autònoma de Barcelona, Barcelona, SpainLaboratory of Nephrology and Transplantation, Vall d’Hebron Research Institute, Vall d’Hebron Barcelona Hospital, Universitat Autònoma de Barcelona, Barcelona, SpainVall d’Hebron for Solid Organ Transplantation Research Group, Vall d’Hebron Research Institute, Vall d’Hebron Barcelona Hospital, Universitat Autònoma de Barcelona, Barcelona, SpainLaboratory of Nephrology and Transplantation, Vall d’Hebron Research Institute, Vall d’Hebron Barcelona Hospital, Universitat Autònoma de Barcelona, Barcelona, SpainKidney Transplant Unit, Nephrology Department, Vall d’Hebron University Hospital, Vall d’Hebron Barcelona Hospital, Universitat Autònoma de Barcelona, Barcelona, SpainLung Transplant Unit, Hospital Universitari i Politècnic La Fe, Valencia, SpainLung Transplant Unit, Hospital Universitari i Politècnic La Fe, Valencia, Spain0Medical Affairs Department, Merck Sharp & Dohme (MSD) Spain, Madrid, Spain0Medical Affairs Department, Merck Sharp & Dohme (MSD) Spain, Madrid, SpainDepartment of Medicine, Universitat Autònoma de Barcelona, Barcelona, SpainLung Transplant Unit, Pulmonology Department, Vall d’Hebron University Hospital, Barcelona, SpainVall d’Hebron for Solid Organ Transplantation Research Group, Vall d’Hebron Research Institute, Vall d’Hebron Barcelona Hospital, Universitat Autònoma de Barcelona, Barcelona, SpainLung Transplant Unit, Pulmonology Department, Vall d’Hebron University Hospital, Barcelona, SpainCentro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, SpainDepartment of Pulmonology, Vall d’Hebron Research Institute, Barcelona, SpainVall d’Hebron for Solid Organ Transplantation Research Group, Vall d’Hebron Research Institute, Vall d’Hebron Barcelona Hospital, Universitat Autònoma de Barcelona, Barcelona, SpainCentro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, SpainIntroductionRisk stratification for CMV infection in lung transplantation (LT) currently relies on determining donor and recipient CMV IgG before transplantation. However, it has been observed that some patients who test positive for CMV-specific humoral response before kidney transplantation (KT) exhibit a weak or absent CMV-specific cellular response. The significance of this observation in LT is still unknown.MethodsThis prospective, multicenter, observational study evaluated the agreement between CMV IgG serology and specific cell-mediated response (specific T cell Enzyme-Linked ImmunoSpot Assay, ELISPOT, against CMV pp65 and IE-1 antigens) in 121 patients on the waiting list for LT.ResultsOne hundred and four (86%) patients were seropositive for CMV. Discordant humoral and cellular immunologic responses were observed, 29% of seropositive patients had a weak ELISPOT response to IE-1 and 39% to pp65. In 22% of seropositive patients, there was a weak or no response to both antigens. All seronegative patients did not respond to either antigen.ConclusionsTherefore, over 20% of CMV seropositive LT candidates showed weak CMV-specific cellular immune responses despite detectable serological memory against CMV. This may be important in assessing the risk of developing a CMV infection after transplantation.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1445553/fullELISPOTinfection riskimmunocompromisedpre-transplantserologyCMV cell-mediated immunity |
| spellingShingle | Meritxell Boada-Pérez Meritxell Boada-Pérez Meritxell Boada-Pérez Cristina Berastegui Cristina Berastegui Cristina Berastegui Marta Erro Marta Erro Piedad Ussetti Elena Crespo Elena Crespo Laura Donadeu Laura Donadeu Oriol Bestard Oriol Bestard Oriol Bestard Gabriel Anguera Amparo Solé Ricardo Ponz Mir Brian Molloy Eva Revilla-López Eva Revilla-López Víctor Monforte Víctor Monforte Víctor Monforte Susana Gómez-Ollés Susana Gómez-Ollés Susana Gómez-Ollés Discordance between humoral and cellular immune responses to cytomegalovirus infection in CMV seropositive patients awaiting lung transplantation Frontiers in Immunology ELISPOT infection risk immunocompromised pre-transplant serology CMV cell-mediated immunity |
| title | Discordance between humoral and cellular immune responses to cytomegalovirus infection in CMV seropositive patients awaiting lung transplantation |
| title_full | Discordance between humoral and cellular immune responses to cytomegalovirus infection in CMV seropositive patients awaiting lung transplantation |
| title_fullStr | Discordance between humoral and cellular immune responses to cytomegalovirus infection in CMV seropositive patients awaiting lung transplantation |
| title_full_unstemmed | Discordance between humoral and cellular immune responses to cytomegalovirus infection in CMV seropositive patients awaiting lung transplantation |
| title_short | Discordance between humoral and cellular immune responses to cytomegalovirus infection in CMV seropositive patients awaiting lung transplantation |
| title_sort | discordance between humoral and cellular immune responses to cytomegalovirus infection in cmv seropositive patients awaiting lung transplantation |
| topic | ELISPOT infection risk immunocompromised pre-transplant serology CMV cell-mediated immunity |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1445553/full |
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