Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures

Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures

Summary: The vascular repair, a pivotal element of the spinal cord (SC) injury response, has been notably neglected in zebrafish research. Here, we detail the extraction and dissection of whole-mount zebrafish SCs and the optimized whole-mount immunofluorescence staining and clearing protocols for t...

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Bibliographic Details
Main Authors: Mariana Rebocho da Costa, Ana Ribeiro, Raquel Quitéria, Dalila Neves-Silva, Leonor Saúde
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Microscopy
Model Organisms
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724006567
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